FastQCFastQC Report
Fri 17 Jun 2016
SRR1526697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222443
Sequences flagged as poor quality0
Sequence length64
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT20670.9292268131611244No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11280.5070961999253741No Hit
GAATTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7680.34525698718323344No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7190.3232288721155532No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6710.3016503104166011No Hit
GAATTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG5970.26838336113071665No Hit
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5590.25130033311904626No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC5240.23556596521356032No Hit
GAATTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.22118025741425895No Hit
GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4790.21533606362079274No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4640.2085927630898702No Hit
GAATTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCGAAGGTCC4470.20095035582149137No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3990.17937179412253926No Hit
GAATTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3660.16453653295450968No Hit
GAATTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.14205886451810126No Hit
GAATTAGGGATGCAACTTAAGTGGAACCAAAAAAAAATTCAAAGATGATTTTGCTTGGATCTAT3060.1375633308308196No Hit
GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.13666422409336323No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2950.13261824377480974No Hit
GAATTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2590.11643432250059566No Hit
GAATTAGGGGAGTGCAGTGCTGAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.11373700228822665No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2510.11283789555077031No Hit
GAATTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGAG2300.10339727480747876No Hit
GAATTAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.10204861470129425No Hit
GAATTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2260.10159906133256609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA258.4746625E-658.19683558
CTCGGCA150.004619852658.19683554
ACGTCTT201.9734782E-458.19683558
GACGCGT150.004619852658.19683558
ATCGGGT201.9734782E-458.19683554
AATCGGG201.9734782E-458.19683553
ATCTACG150.004628136858.1705147
CTACGGG150.004628136858.1705149
ACCCGAT150.00463643258.14421542
CCTATTC150.00463643258.14421543
ATAAGCG201.9823256E-458.1442141
TGATAAG201.9845425E-458.13107339
GTTAGGA150.004640583458.13107340
GCGTAGA202.000115E-458.03925737
CGGGCAA351.6303602E-858.0130834
CTAATAG150.004678073358.0130833
CCGTATG150.00468225358.029
TATAGTC150.00468225358.030
TAGGTTT150.00468643557.98692728
CGCGTAT150.00468643557.98692728
Produced by FastQC (version 0.11.5)