FastQCFastQC Report
Fri 17 Jun 2016
SRR1526696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221770
Sequences flagged as poor quality0
Sequence length64
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT19450.8770347657482978No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10560.4761690039229833No Hit
GAATTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7300.32916986066645626No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6760.3048203093294855No Hit
GAATTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG6040.27235424088019117No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT5280.23808450196149164No Hit
GAATTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5210.23492807864003246No Hit
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4850.21869504441538531No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4850.21869504441538531No Hit
GAATTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCCGAAGGTCC4560.2056184335121973No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4530.2042656806601434No Hit
GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4250.1916399873743067No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4110.18532714073138837No Hit
GAATTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.13617712044009558No Hit
GAATTAGGGATGCAACTTAAGTGGAACCAAAAAAAAATTCAAAGATGATTTTGCTTGGATCTAT3010.13572620282274428No Hit
GAATTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.1316679442665825No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2740.12355142715425893No Hit
GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.11994408621544843No Hit
GAATTAGGGGAGTGCAGTGCTGAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.10551472246020652No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC2270.10235829913874735No Hit
GAATTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGAG2230.10055462866934212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT150.004510428358.5500345
TGCGAAA258.209168E-658.50984244
TAAGCGC201.9283246E-458.46970442
TACTTTC351.5323167E-858.469743
ATAAGCG201.9305063E-458.45633341
TAGCGCT150.00456796758.3629326
TTGAAAG150.004584506658.3096922
TACCTAG201.9812297E-458.1505650
CGCACGA201.9812297E-458.1505657
TTACCTA201.9812297E-458.1505649
TAGTCTC201.9812297E-458.1505649
CCCTAAG150.00463439358.15055549
CCGTAGT351.599983E-858.15055558
AATGACG150.00463439358.15055550
TGTCGTG150.00463439358.15055551
AACCGAT201.983459E-458.13733752
ATATCGT150.00463856858.13733355
AAATTCG303.6868914E-758.13733348
TAACTCG150.00463856858.13733356
ACGGTCT150.00463856858.13733347
Produced by FastQC (version 0.11.5)