FastQCFastQC Report
Fri 17 Jun 2016
SRR1526695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196743
Sequences flagged as poor quality0
Sequence length56
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG22211.1288838738862372No Hit
GAATTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11660.5926513268578806No Hit
GAATTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA8280.4208536008905018No Hit
GAATTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC7090.3603686026948862No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC6730.3420706200474731No Hit
GAATTAGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA6520.3313967968364821No Hit
GAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5640.28666839480947226No Hit
GAATTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5510.28006078996457306No Hit
GAATTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.2490558749231231No Hit
GAATTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4200.21347646421981978No Hit
GAATTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3850.19568675886816814No Hit
GAATTAGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCC3730.18958743131903041No Hit
GAATTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC3580.18196327188260827No Hit
GAATTAGGGATGCAACTTAAGTGGAACCAAAAAAAAATTCAAAGATGATTTTGCTT3580.18196327188260827No Hit
GAATTAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3230.1641735665309566No Hit
GAATTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.15095835684115827No Hit
GAATTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2780.14130108822169024No Hit
GAATTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2210.1123292823632861No Hit
GAATTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2060.10470512292686399No Hit
GAATTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC2050.10419684563110251No Hit
GAATTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2040.10368856833534103No Hit
GAATTAGGGGAGTGCAGTGCTGAATTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2040.10368856833534103No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAA203.8911094E-450.71917350
TCACAAC252.0112284E-550.28923846
ATAGGAT301.0026724E-650.28923447
TTGTAGT150.00823075450.28923444
AATATAG301.0026724E-650.28923444
ATATAGG301.0026724E-650.28923445
GTTTCGA150.00827263150.22472842
ATGTTGG301.0115928E-650.22472842
ACTAATG355.097172E-850.1989837
ATATGGC150.00828942650.1989739
TTAGTAT150.00828942650.1989740
TATTCCC150.00829783350.18610436
TGACTAA355.1177267E-850.1732535
TAGGATC150.00831466650.1603934
AATGATC204.1358927E-450.09621433
CCTATAG150.00839072950.04498732
TGATCTA150.00839072950.04498732
GACGAAT252.0762976E-550.0194231
CGTTAAA402.6539055E-950.01941731
TTGATAT150.00840770250.01941331
Produced by FastQC (version 0.11.5)