FastQCFastQC Report
Fri 17 Jun 2016
SRR1526694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162986
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC5310.3257948535457033No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4680.2871412268538402No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3150.1932681334593155No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2810.17240744603831004No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2470.15154675861730457No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC2260.13866221638668352No Hit
AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.11473378081553016No Hit
AGTCGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.1141202311855006No Hit
AGTCGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.10614408599511614No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC1730.10614408599511614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGA150.004500268558.57631756
TTTCGAC150.004500268558.57631757
TCTAGCC201.9095112E-458.57631758
TTCGACG258.144363E-658.57631758
AGTATAA258.573939E-658.0708849
TATCTCA150.0046574258.0708848
TAAGTAG201.9992207E-458.0351153
GTTAATT201.9992207E-458.0351145
TGGTCCG201.9992207E-458.0351154
ACTATAC150.004674496658.01724236
ATACTAC150.004674496658.01724239
TAGGCCC150.004674496658.01724241
CTTTAAG150.004674496658.01724239
AATCGGA150.004674496658.01724239
TGAATAC150.00468019957.99938634
CCGTTAC150.00468019957.99938633
GATTAGA202.0114308E-457.96370728
GGCATTA303.78177E-757.9102678
GGATTTG150.004708789857.9102678
AGACCAG150.004720262357.87469523
Produced by FastQC (version 0.11.5)