FastQCFastQC Report
Fri 17 Jun 2016
SRR1526693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236988
Sequences flagged as poor quality0
Sequence length64
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC17740.7485611085793373No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC15770.6654345367697942No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC10070.4249160295036036No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT8520.3595118740189377No Hit
AGTCGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT6300.26583624487315816No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC6230.26288250881901193No Hit
AGTCGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT5830.24600401708103364No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC5800.24473813020068524No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC5380.22701571387580807No Hit
AGTCGTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG5080.21435684507232433No Hit
AGTCGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA4600.19410265498675036No Hit
AGTCGTGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC4040.17047276655358076No Hit
AGTCGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC3840.16203352068459165No Hit
AGTCGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3720.15696997316319813No Hit
AGTCGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC3570.15064053876145628No Hit
AGTCGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA3420.14431110435971442No Hit
AGTCGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3330.1405134437186693No Hit
AGTCGTGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC3100.1308083109693318No Hit
AGTCGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC3070.1295424240889834No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3050.1286984995020845No Hit
AGTCGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC2990.12616672574138774No Hit
AGTCGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2970.12532280115448885No Hit
AGTCGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTC2880.12152514051344372No Hit
AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.12068121592654481No Hit
AGTCGTGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2670.11266393235100512No Hit
AGTCGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG2580.10886627170996No Hit
AGTCGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTTTCCAAAGGTCC2500.10549057336236435No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2490.10506861106891487No Hit
AGTCGTGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCGTGTTCATTCAGC2490.10506861106891487No Hit
AGTCGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT2440.10295879960166761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC150.004617832658.2044658
ACCGCTA150.004617832658.2044658
CGTTGAT150.004617832658.2044656
TAGCGGA150.004617832658.2044656
TTCGTGC150.004617832658.2044658
TATCGGG201.9766128E-458.17975650
GTTAATT201.984924E-458.13040545
GAATAGA150.004645070558.11808440
TAAAACG201.9870061E-458.11808439
TATGCCA201.9870061E-458.11808443
GTATATC150.004656780558.08114238
TATGTCA150.004656780558.08114237
CGTTAAA258.66355E-657.98287231
CAATTGA150.004692042257.97060429
ACGGACA150.004692042257.97060429
ATTATGG150.004692042257.97060430
ATGAATT150.00470777857.9216124
CCGGTAG150.00470777857.9216124
CCGTAAA303.8006692E-757.88492620
AAATAGG202.0332541E-457.84827816
Produced by FastQC (version 0.11.5)