FastQCFastQC Report
Fri 17 Jun 2016
SRR1526690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329370
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6960.21131250569268603No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4630.14057139387315176No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4620.14026778395117953No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4320.13115948629201202No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.10474542308042625No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAC150.004659075358.07947
GTACGAT150.00467880158.01706340
AATATCT150.004712761457.9111930
TAGTATG202.0235192E-457.9111928
GACGTAG258.821829E-657.8144810
AGGGTCG331000.057.0016481
GGTCGGG322300.056.978813
TCGGGAT22650.056.951175
GGGTCGG329600.056.8715482
CGGGATA2350.056.869296
GTCGGGA92650.056.757214
GTCGGGG196000.056.6974144
CGGGATC7400.056.5351686
TCGGGGG97750.056.292575
TCGGGTA2900.056.1019485
TCGGGAG35050.056.0333445
TCGGGAC16750.055.677295
TCGGGGC32450.055.419655
TCGGGGA47700.055.242945
TCGGGCA7950.055.1820375
Produced by FastQC (version 0.11.5)