FastQCFastQC Report
Fri 17 Jun 2016
SRR1526688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences486780
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC21190.4353095854390074No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT17030.34985003492337396No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC15380.31595381897366365No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC13920.28596080364846543No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC13370.274662064998562No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG12380.2543243354287358No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC11320.23254858457619457No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC11030.2265910678335182No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC9380.1926948518838079No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT9310.1912568306010929No Hit
AGGGTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC7210.1481161921196434No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC5770.11853404001807798No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.11668515551173016No Hit
AGGGTCGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC5300.10887875426270595No Hit
AGGGTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG5160.10600271169727597No Hit
AGGGTCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGC5120.10518098525001028No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA5040.10353753235547887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA201.948607E-458.36038625
GCCGAAC150.004596577558.2816521
TGCGTAA150.00465540858.09475757
GCAACGT150.00466685958.05872346
TACAGGT150.004697497557.96284538
CGAAACT150.004709025457.92697535
CGTAGTA150.00478641857.68895717
AGGGTCG489800.056.8348961
TCGGGAT33800.056.678535
GGGTCGG488250.056.635852
GTCGGGG295100.056.585514
GGTCGGG479550.056.5606883
TCGGGGG143000.056.0319335
GTCGGGA134400.055.682954
TCGGGGA75900.055.581835
CGGGATA4550.055.249666
CGGGATC9950.055.176266
CGGGATT11300.054.7213866
TCGGGAG51650.054.7129675
TCGGGGC49750.054.5954635
Produced by FastQC (version 0.11.5)