FastQCFastQC Report
Fri 17 Jun 2016
SRR1526687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450141
Sequences flagged as poor quality0
Sequence length56
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC44090.9794708769030148No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC25870.5747088134606713No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT17260.3834354124596515No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC12050.2676939003556663No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC11670.2592521010083507No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA11210.24903308074581076No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG10370.2303722611359552No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7730.17172397093355193No Hit
AGGGTCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC7490.1663923081878789No Hit
AGGGTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA6370.14151121537473815No Hit
AGGGTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC6100.135513094785856No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5910.1312921951121982No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC5670.12596053236652516No Hit
AGGGTCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5320.11818519086241866No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG5180.11507505426077606No Hit
AGGGTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT5160.11463074903196999No Hit
AGGGTCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT4960.11018769674390914No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC4620.10263450785420569No Hit
AGGGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT4600.1021902026253996No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA4590.10196805001099656No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG4520.10041298171017526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATTC150.008014847550.639348
TCGGTTA150.00826459350.2477647
TTCGTAA150.00827191750.236543
TAGTTAG150.00827191750.236545
TCGTAAC150.00827191750.236544
GTAACGT150.00827191750.236546
TCACGTT150.00842684450.0012331
GTCGACA204.194457E-449.96779629
GACGAGT150.00844915249.96779629
TATCGTG150.00845659749.95666528
GTATCGT150.00846032249.951127
CCGTTGA204.2501016E-449.8345220
TTAGGTC150.00855383549.81237413
ATTGTCG204.2594326E-449.81237413
CACCTAA204.2594326E-449.81237414
TCGGATA204.2594326E-449.8123745
TGATTCG204.2594326E-449.81237415
GTCGGTA451.4188117E-1049.8123744
AGGGTCG452700.049.2126921
GGGTCGG451800.049.0791932
Produced by FastQC (version 0.11.5)