##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526687_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 450141 Sequences flagged as poor quality 0 Sequence length 56 %GC 59 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.24598958992849 39.0 38.0 40.0 35.0 40.0 2 37.99361533386206 39.0 38.0 40.0 35.0 40.0 3 37.949680211311566 39.0 38.0 40.0 35.0 40.0 4 37.982745406439314 39.0 38.0 40.0 35.0 40.0 5 36.80310391632844 39.0 36.0 39.0 32.0 40.0 6 38.471567797645626 39.0 38.0 40.0 36.0 40.0 7 38.53917772431305 39.0 38.0 40.0 37.0 40.0 8 38.47921429063338 39.0 38.0 40.0 36.0 40.0 9 38.482237787715405 39.0 38.0 40.0 36.0 40.0 10 37.80632512923729 39.0 38.0 40.0 35.0 40.0 11 37.810928131407714 39.0 38.0 40.0 35.0 40.0 12 37.715991211642574 39.0 38.0 40.0 34.0 40.0 13 37.47282073839086 39.0 38.0 40.0 33.0 40.0 14 37.7157957173419 39.0 38.0 40.0 34.0 40.0 15 37.69472676339191 39.0 38.0 40.0 34.0 40.0 16 37.53455028535503 39.0 38.0 40.0 33.0 40.0 17 37.409145134524515 39.0 37.0 40.0 33.0 40.0 18 37.30730371150373 39.0 37.0 40.0 33.0 40.0 19 37.20164792809364 39.0 36.0 40.0 33.0 40.0 20 37.12373678469635 39.0 36.0 40.0 33.0 40.0 21 36.96906746997052 38.0 36.0 40.0 33.0 40.0 22 36.81177897592088 38.0 36.0 40.0 32.0 40.0 23 36.646579627272345 38.0 36.0 40.0 32.0 40.0 24 36.54042622200599 38.0 36.0 40.0 31.0 40.0 25 36.33120066823506 38.0 35.0 40.0 31.0 40.0 26 35.79410673544512 38.0 35.0 39.0 30.0 40.0 27 35.528940931841355 38.0 35.0 39.0 29.0 40.0 28 35.207463883538715 38.0 35.0 39.0 28.0 40.0 29 35.04984438209361 38.0 34.0 39.0 28.0 40.0 30 34.738206473082876 38.0 33.0 39.0 27.0 40.0 31 33.90990822875499 37.0 33.0 39.0 25.0 40.0 32 33.70724062016124 37.0 33.0 39.0 23.0 40.0 33 33.45144299230685 37.0 33.0 39.0 23.0 40.0 34 33.64028160065402 37.0 33.0 39.0 24.0 40.0 35 33.342257203853904 37.0 32.0 39.0 23.0 40.0 36 33.08505779300264 37.0 32.0 39.0 19.0 40.0 37 32.805687551233945 36.0 32.0 39.0 18.0 40.0 38 32.578658686944756 36.0 32.0 39.0 17.0 40.0 39 32.39051541628068 36.0 31.0 39.0 14.0 40.0 40 32.015157472880716 36.0 31.0 39.0 10.0 40.0 41 31.535825441361705 36.0 30.0 39.0 4.0 40.0 42 31.31840467764545 36.0 30.0 39.0 4.0 40.0 43 31.111793860146044 36.0 30.0 39.0 4.0 40.0 44 30.792436147784805 35.0 30.0 39.0 4.0 40.0 45 30.37099930910537 35.0 29.0 38.0 4.0 40.0 46 29.55459955880491 35.0 28.0 38.0 4.0 40.0 47 29.22201487978211 35.0 27.0 38.0 4.0 40.0 48 28.863433901821875 34.0 26.0 38.0 4.0 40.0 49 28.59336074696595 34.0 25.0 38.0 4.0 40.0 50 28.099899809170903 34.0 24.0 38.0 4.0 39.0 51 27.202532095498967 33.0 18.0 38.0 4.0 39.0 52 26.846552524653386 33.0 15.0 38.0 4.0 39.0 53 26.4919902874877 33.0 9.0 37.0 4.0 39.0 54 26.095525624193307 33.0 4.0 37.0 4.0 39.0 55 25.694149166594467 33.0 4.0 37.0 4.0 39.0 56 15.455428410209246 19.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.205431611218664 1 2 -1.2612112529674846 1 3 -1.6463908120920365 1 4 -1.5694217283627978 1 5 -2.3464874786727137 1 6 -1.5957725975013872 1 7 -1.3141861658747374 1 8 -1.6739643587622055 1 9 -1.4843545335820139 1 10 -1.3022739111297668 1 11 -1.1343135338037555 1 12 -1.0627212364533563 1 13 -0.9654795140741754 1 14 -1.1797147285360268 1 15 -1.1902730119576788 1 16 -0.5775318176754141 1 17 -0.5267111708786203 1 18 -0.4898084363747941 1 19 -0.7003721177990698 1 20 -0.7406724762776022 1 21 -0.6944479136412411 1 22 -0.32847735543736434 1 23 -0.48277116655535934 1 24 -0.5913987817189863 1 25 -0.7278371181093632 1 26 -0.5229430469731469 1 27 -1.068827758600591 1 28 -0.2975688867778672 1 29 -0.1705344091814709 1 30 -0.5039017475382366 1 31 -0.3464254164084366 1 32 0.044120169132057185 1 33 -0.41173610006732275 1 34 -1.0064086361671372 1 35 -1.2797854909955433 1 36 0.18772468783714658 1 37 -0.048776030135464055 1 38 0.4447856123259726 1 39 0.6311926591183266 1 40 0.3145309507193019 1 41 0.7071210008923678 1 42 0.004812828242396705 1 43 0.6234820571910511 1 44 0.4484848669157486 1 45 0.39911589557987526 1 46 0.9803924114767604 1 47 0.7154507702462993 1 48 0.8352310317772726 1 49 1.3742761452513257 1 50 1.0136432593088749 1 51 1.8146125166121543 1 52 2.1203535154796214 1 53 1.6637882401651112 1 54 1.3092650271664326 1 55 1.4352772926866848 1 56 1.5621687949429859 2 1 -0.12816623905037972 2 2 -0.16393350691760133 2 3 -0.18586825355405523 2 4 -0.19960924026075588 2 5 -0.24176160774181454 2 6 -0.07511773361408558 2 7 0.04772607474966151 2 8 -0.03618594642640005 2 9 0.03513432992455279 2 10 -0.2439738920930239 2 11 -0.16750885076549338 2 12 -0.34315622598314377 2 13 0.14876472731170054 2 14 -0.007574998857748483 2 15 -0.06391664416784693 2 16 -0.0432656840374932 2 17 -0.054742771868532714 2 18 0.14278246406305328 2 19 -0.07427750519674703 2 20 0.18279982526437522 2 21 0.045652981085581246 2 22 0.01128087795307664 2 23 0.03745537066146909 2 24 0.08652335801069455 2 25 -0.209300091648295 2 26 0.39955362159719243 2 27 0.5824191479290093 2 28 0.3003989411299983 2 29 0.3114996665847585 2 30 0.2877649191284277 2 31 0.8471306467935449 2 32 0.9571698550258319 2 33 0.6314535058721376 2 34 0.7206996828886361 2 35 1.3144463765087409 2 36 0.5762407738832138 2 37 1.080078909898802 2 38 1.0467511558249214 2 39 0.46876166730412905 2 40 0.17349249650266785 2 41 0.712665451682394 2 42 -0.22803030901250665 2 43 0.44722563229099066 2 44 0.8911128889983679 2 45 0.5917201217363584 2 46 0.9203552898319849 2 47 1.2239459158773656 2 48 0.5646951475206592 2 49 1.2225771161251124 2 50 0.47009671447735 2 51 0.6217465352681621 2 52 0.7387382480134121 2 53 0.6493203170735455 2 54 0.7363067176290237 2 55 0.959691912903704 2 56 0.5001804073549394 3 1 -0.33651004259120754 3 2 -0.1516034098302228 3 3 -0.27315551797438786 3 4 -0.24697074797063578 3 5 -1.2261933610256506 3 6 -0.25890985240334885 3 7 -0.12546067567866004 3 8 -0.012199652879857581 3 9 -0.015825121817307775 3 10 -0.18325430328662407 3 11 -0.4457841220390506 3 12 -0.04576045213963198 3 13 -0.013028533682017951 3 14 -0.24520492461446253 3 15 -0.17439065901650252 3 16 0.20011524114811863 3 17 -0.430436661074701 3 18 -0.16823980892381485 3 19 -0.2926270197598555 3 20 0.06285693548710469 3 21 0.26653247851562156 3 22 0.3266206837783159 3 23 -0.05649665675143467 3 24 0.4375227869084668 3 25 0.3779471956161231 3 26 0.4630373451837144 3 27 0.3848977315954869 3 28 0.6048820936142931 3 29 0.5483871594459799 3 30 0.965411977951959 3 31 0.8827902698660708 3 32 0.7029436985438267 3 33 0.9791462528738535 3 34 0.7198658736367847 3 35 0.9631556854750443 3 36 1.2513521388175377 3 37 1.0303416169233586 3 38 0.9601777691887179 3 39 1.2037416787261748 3 40 0.8352172252291084 3 41 1.1093759028531487 3 42 1.0425579262816136 3 43 1.3525997042498723 3 44 1.1009358473079054 3 45 1.177351189697525 3 46 1.1489218232414657 3 47 1.285156652599241 3 48 1.0929761298164884 3 49 1.2004526158395628 3 50 1.0942314946029938 3 51 2.2105929087690193 3 52 2.3186868488129555 3 53 2.4030039264396237 3 54 2.428088556578196 3 55 2.6170419985690394 3 56 1.3597178145508284 4 1 -0.27672790751496024 4 2 -0.19407254563269305 4 3 -0.16997469008767752 4 4 -0.17132804644558064 4 5 -0.7479943777286309 4 6 0.13167838289076883 4 7 0.12203387769824303 4 8 0.19128618590010404 4 9 0.14522062981667716 4 10 -0.05663120960470991 4 11 0.10274165864359475 4 12 0.23333903950888413 4 13 0.2553810523746236 4 14 0.02166508264770073 4 15 0.025667438296494538 4 16 -0.03947807766088829 4 17 0.3317129395062679 4 18 -0.03664939498045783 4 19 0.02441001727717662 4 20 0.19796223686691405 4 21 0.006314613591868579 4 22 0.1369910541486874 4 23 0.014759697642176661 4 24 0.32902605488231984 4 25 0.32098278022033355 4 26 0.0022385071299737547 4 27 0.19108509542991214 4 28 0.4721297334109522 4 29 0.6703334194314579 4 30 0.7025797339432458 4 31 1.292318229197953 4 32 1.0616946063143402 4 33 0.9664229413270107 4 34 1.1092050167014165 4 35 0.9081956273777294 4 36 1.22401735305138 4 37 1.4504432872211126 4 38 1.612668691483556 4 39 0.9199363047247218 4 40 1.4523849812203977 4 41 2.044248814980964 4 42 1.6386363576541605 4 43 1.7863542431024477 4 44 1.7385538574749368 4 45 1.675622794635796 4 46 2.1613836968798097 4 47 2.008352004814199 4 48 2.170652237296519 4 49 2.1387823180908327 4 50 2.1789664256124333 4 51 2.1420090307733766 4 52 1.6917587442377915 4 53 2.216802038276949 4 54 1.4766579102455886 4 55 1.8894893478791346 4 56 1.1119327746089258 5 1 -0.3584784231637812 5 2 -0.38627653331690937 5 3 -0.43472224636065704 5 4 -0.5307935652331395 5 5 -0.9211029180688115 5 6 -0.2528252490704901 5 7 -0.08201957042300023 5 8 -0.039688614566223634 5 9 -0.10412023647334223 5 10 -0.1557254612413317 5 11 -0.004399703865004767 5 12 0.07245062890295628 5 13 0.4838807385014263 5 14 -0.25622329806553523 5 15 -0.04844848553693737 5 16 0.03541505598930428 5 17 -0.06287648446168248 5 18 -0.6501369745697332 5 19 0.039188960869680045 5 20 0.16753148327232026 5 21 -0.10207028462353662 5 22 -0.23289505472047267 5 23 -0.11190756687819459 5 24 -0.26360240893258435 5 25 -0.08607787203805373 5 26 0.13525497799955133 5 27 0.06408303283462402 5 28 0.3621246516544545 5 29 0.5455613511565645 5 30 0.3991450644068806 5 31 0.6733842023543204 5 32 0.19780198083979172 5 33 0.304347791116264 5 34 0.470493989289821 5 35 0.4896561839795268 5 36 0.5384166595410775 5 37 0.0319995004157434 5 38 0.6990525733476645 5 39 0.36750752404735465 5 40 0.4467711349285821 5 41 0.8284415630155202 5 42 0.74775661592696 5 43 1.0513947476909813 5 44 1.047040390820225 5 45 1.570280971779848 5 46 1.3770773566412657 5 47 1.8089737726676063 5 48 1.304780716057767 5 49 1.504118760263431 5 50 1.5876711755601214 5 51 2.0656391984713416 5 52 2.3188235814721203 5 53 2.189330660523087 5 54 2.124462292513247 5 55 2.1933245793979808 5 56 0.7257910234954235 6 1 -0.198789099141365 6 2 -0.2548884339848172 6 3 -0.40218080925031785 6 4 -0.2927451156887386 6 5 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1.070684523030856 6 46 1.8533321670891496 6 47 1.915329997299441 6 48 1.7731750499642587 6 49 1.779469381966301 6 50 1.9341462431110905 6 51 2.2441024285189464 6 52 2.620744251484169 6 53 1.9682781520719033 6 54 2.4358634926395766 6 55 2.4161667499188546 6 56 1.8343697042922305 7 1 -0.2644250105585968 7 2 -0.357940043493592 7 3 -0.3734583717891766 7 4 -0.4490324835559605 7 5 0.03433663315023239 7 6 -0.11683646859437857 7 7 -0.29712637166002764 7 8 -0.1412037297581321 7 9 -0.09086297854415903 7 10 0.1333211189614829 7 11 -0.16976781452594736 7 12 -0.08613901940812241 7 13 0.017023883324384315 7 14 -0.2513027696930905 7 15 -0.2300225751492917 7 16 -0.446634904454676 7 17 -0.5377284490770293 7 18 -0.46908430513813926 7 19 -0.6953002870865888 7 20 -0.6774808629986282 7 21 -0.15960031123394458 7 22 -0.12793377166722308 7 23 -0.10240813025172457 7 24 0.10834398667550005 7 25 -0.09327586786669428 7 26 0.1996593603264074 7 27 -0.20821816822979144 7 28 0.11242431842500622 7 29 0.2512526224628715 7 30 0.7036065032868422 7 31 0.5032154300291509 7 32 0.7194259350027679 7 33 0.6778824206082703 7 34 0.5540650582611306 7 35 0.7404532975891982 7 36 0.9425053129584811 7 37 0.5821936844830589 7 38 0.7739692659854569 7 39 1.1730777299783597 7 40 0.9459860079897382 7 41 1.3277043827599648 7 42 0.997217215739969 7 43 0.8725356390198193 7 44 1.192636487960204 7 45 0.9205195065292067 7 46 1.056469872164005 7 47 1.0607477999492687 7 48 1.1160745573062982 7 49 0.959672184855286 7 50 1.2115174584934998 7 51 2.063277824319396 7 52 2.120911659529323 7 53 1.9681466171792863 7 54 1.841588847783786 7 55 2.1133495116732917 7 56 1.4471668535723747 8 1 -0.938808122444911 8 2 -0.8335723217473259 8 3 -1.0195621013331149 8 4 -1.2714815678426277 8 5 -0.11024090750538562 8 6 -0.4605495963838422 8 7 -0.8351528427009143 8 8 -1.0967775254796734 8 9 -1.2231155820428512 8 10 -0.38027350474959576 8 11 -0.20239491143252764 8 12 -0.384053655431309 8 13 -0.15816721091456998 8 14 -0.44284588773545863 8 15 -0.3154635026617285 8 16 -0.47299051929077507 8 17 -0.3776966427875976 8 18 -0.4313912871316745 8 19 -0.6012280560292851 8 20 -0.5712119459514824 8 21 -0.3863355495246168 8 22 0.08112845579904615 8 23 -0.00436011301476924 8 24 -0.23821631702325874 8 25 -0.02836795220466115 8 26 0.2753018986735043 8 27 0.4577901424458375 8 28 0.2542572841049946 8 29 0.2250893843774051 8 30 0.8033089605792014 8 31 0.6364812177478143 8 32 0.5169942928748199 8 33 0.2621819127336522 8 34 0.8494787965261494 8 35 1.4455269137591031 8 36 1.6362287008413077 8 37 1.298412327072704 8 38 1.3462003232880733 8 39 1.114531072203775 8 40 1.7059639487889982 8 41 1.6732771524721706 8 42 1.214974872334647 8 43 1.2307283235732491 8 44 1.400570105156124 8 45 1.5689574073141053 8 46 1.9869833898370182 8 47 1.6046994749095127 8 48 1.9324701865065244 8 49 2.325701015717648 8 50 2.603478675340572 8 51 2.24216255217042 8 52 2.298436925009238 8 53 2.5000170322000628 8 54 2.756994377974113 8 55 2.6002422423971403 8 56 2.9390229044624387 9 1 -0.22525361769115193 9 2 -0.23189467196614544 9 3 -0.2926643700589082 9 4 -0.21659953152289546 9 5 0.2169762563358546 9 6 -0.07269054914807072 9 7 -0.015380721366838657 9 8 -0.03375876196037808 9 9 0.06911491244882484 9 10 -0.010964183355156365 9 11 0.24025813952577124 9 12 0.08888260896831213 9 13 0.524978319544708 9 14 0.20359004968593553 9 15 0.32443287039526325 9 16 0.26255955868095526 9 17 0.15399509220913643 9 18 0.39520964852906815 9 19 0.19999433946344425 9 20 0.12940176701194872 9 21 0.2616723985613092 9 22 0.5355527226132679 9 23 0.503474788137197 9 24 0.4020573385932167 9 25 0.5552630151478226 9 26 0.9286798351894276 9 27 0.8955259440455166 9 28 0.8926319508387337 9 29 1.2168394724100011 9 30 1.062036763788619 9 31 1.555868510871214 9 32 1.2095970394918538 9 33 1.403298166066314 9 34 1.3760394887138787 9 35 1.5353201629165056 9 36 1.9767262107764196 9 37 2.007263375918221 9 38 1.7918967868928846 9 39 1.898373317789563 9 40 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0.37494835307440155 120 52 0.2596074440013858 120 53 0.3828120198897693 120 54 0.6923132819293301 120 55 0.14974676842023982 120 56 0.46696298212140164 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 172.0 5 27.0 6 37.0 7 75.0 8 151.0 9 214.0 10 228.0 11 404.0 12 471.0 13 695.0 14 1114.0 15 1402.0 16 1896.0 17 2510.0 18 3356.0 19 3763.0 20 3901.0 21 4926.0 22 4958.0 23 5480.0 24 7169.0 25 8574.0 26 8453.0 27 9286.0 28 10508.0 29 11330.0 30 12925.0 31 15245.0 32 18095.0 33 22519.0 34 29355.0 35 39108.0 36 53345.0 37 67328.0 38 66845.0 39 34276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.3786391375147 0.4791831892673629 0.09374840327808398 0.04842926993986329 8 99.34113160923881 0.4633189189669654 0.10444119516281715 0.09110827663139369 9 97.89421536807356 1.0912136419477452 0.48207117325460247 0.5324998167240931 10 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25.036821795837305 23 31.31796481546893 19.125118573957938 24.893533359547344 24.663383251025788 24 31.925288377797127 19.12580203686259 22.892042727902883 26.056866857437395 25 29.23291944787621 19.06546915412715 22.896650105323037 28.804961292673603 26 27.163847333674173 18.3426323160024 24.879409607219863 29.61411074310356 27 26.421461185184526 17.526797583215888 25.227295967136005 30.82444526446358 28 28.41726538717259 21.761850441063 22.01623716670113 27.804647005063277 29 30.98903984015877 20.69236348749349 22.29389070099634 26.0247059713514 30 31.027517460091218 18.607468643101484 24.50915763968073 25.855856257126568 31 32.42215265321583 18.958990184828263 23.115453603538363 25.503403558417542 32 35.55690231789186 18.572545804154515 22.528759193695368 23.341792684258255 33 35.21569658732051 18.469431190370596 23.73565049954425 22.579221722764643 34 29.825640751291754 20.890788071705295 27.979733133607066 21.30383804339588 35 28.363151451361496 24.377788828407752 26.79973508220702 20.45932463802373 36 29.52871311866721 25.413708946999748 22.646131057283466 22.41144687704958 37 30.743539459329217 25.208992885206182 21.830971775084166 22.21649588038044 38 32.13232651731089 24.40152406291782 21.52258974627968 21.943559673491617 39 32.97789905964373 21.253034217713353 21.414933598922754 24.354133123720157 40 29.508134441762763 20.624385447394296 22.374184321086975 27.49329578975597 41 26.606287913669707 21.24730106080045 23.819507463153613 28.326903562376227 42 25.810280921232064 19.578193394349384 24.636608735697965 29.974916948720587 43 25.69735817998014 18.351837107148928 26.8301738251371 29.120630887733835 44 27.058397294831195 18.133084643873115 25.032875315334657 29.775642745961033 45 27.764840106036708 18.802948446920126 24.631835620728612 28.800375826314557 46 27.26202624559142 20.362264353617274 24.10105268811429 28.274656712677015 47 26.52810394890313 19.245949570274984 26.05819365497973 28.167752825842157 48 28.302182639237333 18.727376173750987 27.838282201992687 25.132158985018993 49 29.573210327145706 18.537584809332948 25.709935287400075 26.17926957612127 50 25.685331277992994 19.30780140572732 26.554668542762204 28.45219877351748 51 25.903668430177085 19.915423175304284 27.116996447594175 27.063911946924456 52 26.060792084975386 19.596900127929096 26.524787102237973 27.817520684857545 53 25.831834079039638 18.76513724058046 28.398303508815165 27.00472517156473 54 26.29336524458393 18.487677666264013 26.825771345352734 28.39318574379932 55 26.563673329403088 19.31909628278037 24.3915779185991 29.72565246921744 56 26.69224580174228 20.298433203271202 23.12767678850373 29.88164420648279 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 3.0 8 6.5 9 10.0 10 13.0 11 16.0 12 51.5 13 176.0 14 265.0 15 274.5 16 284.0 17 367.0 18 450.0 19 569.0 20 688.0 21 848.5 22 1113.5 23 1218.0 24 1382.5 25 1547.0 26 1803.5 27 2060.0 28 2393.5 29 3261.5 30 3796.0 31 4168.0 32 4540.0 33 4845.0 34 5150.0 35 4909.5 36 4669.0 37 5054.0 38 5013.5 39 4588.0 40 4846.5 41 5105.0 42 5614.0 43 6123.0 44 6357.5 45 6592.0 46 7884.0 47 9331.0 48 9486.0 49 10230.0 50 10974.0 51 11614.0 52 12254.0 53 14124.5 54 16985.5 55 17976.0 56 20484.0 57 22992.0 58 25691.0 59 28390.0 60 31428.5 61 34467.0 62 40747.0 63 47755.5 64 48484.0 65 41652.5 66 34821.0 67 31303.5 68 27786.0 69 25237.5 70 22689.0 71 19385.0 72 14082.5 73 12084.0 74 10214.0 75 8344.0 76 7219.0 77 6094.0 78 4814.5 79 2846.0 80 2157.0 81 1780.5 82 1404.0 83 1127.0 84 850.0 85 627.5 86 405.0 87 295.5 88 125.5 89 65.0 90 49.5 91 34.0 92 21.0 93 8.0 94 8.0 95 8.0 96 5.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.028213382029186412 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 4.443052288060852E-4 21 0.0 22 0.0 23 0.0 24 0.008219646732912577 25 0.021548803597095132 26 0.06020335850322455 27 0.06375780033367322 28 0.09596992942211441 29 0.1543960670101146 30 0.24814447028819858 31 0.29901741898649536 32 0.29501867192724057 33 0.31812254382515703 34 0.33922704219344607 35 0.3776594444851724 36 0.41742476246331706 37 0.42653301965384177 38 0.47274076344967464 39 0.5180598967878954 40 0.5911481069264963 41 0.6093646213075459 42 0.6280254409174014 43 0.6720116585692039 44 0.6657913853659186 45 0.6944490726239112 46 0.729771338313995 47 0.7686480458345274 48 0.823964046820885 49 0.7888639337452044 50 0.8495115974772349 51 0.8181880788464059 52 0.8441799347315618 53 0.8459571556467862 54 1.6097178439644466 55 0.8948307308154556 56 0.8761699112056 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 450141.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.46864852371917 #Duplication Level Percentage of deduplicated Percentage of total 1 69.357511423844 25.293747065960037 2 11.497028149124805 8.38562157275476 3 5.71532083837858 6.252900805653491 4 3.3503151293497986 4.8872585958382615 5 2.221996982317749 4.05166134844553 6 1.579717916841741 3.4566106485553973 7 1.1419063672806888 2.915064736874949 8 0.7998407239032019 2.333528818798638 9 0.6338465426040061 2.080397410218007 >10 3.3638528699771713 22.391258922741763 >50 0.20756767971570209 5.256080959856203 >100 0.1213846080208784 8.546913979233052 >500 0.005462307360939528 1.2158021102661443 >1k 0.0042484612807307445 2.93315302480374 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 4409 0.9794708769030148 No Hit AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 2587 0.5747088134606713 No Hit AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 1726 0.3834354124596515 No Hit AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 1205 0.2676939003556663 No Hit AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 1167 0.2592521010083507 No Hit AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA 1121 0.24903308074581076 No Hit AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG 1037 0.2303722611359552 No Hit AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 773 0.17172397093355193 No Hit AGGGTCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 749 0.1663923081878789 No Hit AGGGTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA 637 0.14151121537473815 No Hit AGGGTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 610 0.135513094785856 No Hit AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 591 0.1312921951121982 No Hit AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 567 0.12596053236652516 No Hit AGGGTCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 532 0.11818519086241866 No Hit AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 518 0.11507505426077606 No Hit AGGGTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT 516 0.11463074903196999 No Hit AGGGTCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT 496 0.11018769674390914 No Hit AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC 462 0.10263450785420569 No Hit AGGGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT 460 0.1021902026253996 No Hit AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCA 459 0.10196805001099656 No Hit AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG 452 0.10041298171017526 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 2.221526144030426E-4 0.0 0.0 0.0 0.0 8 2.221526144030426E-4 0.0 0.0 0.0 0.0 9 2.221526144030426E-4 0.0 0.0 0.0 0.0 10 0.001777220915224341 0.0 0.0 0.0 0.0 11 0.003332289216045639 0.0 0.0 0.0 0.0 12 0.0037765944448517243 0.0 0.0 0.0 0.0 13 0.0053316627456730225 0.0 0.0 0.0 0.0 14 0.006442425817688236 0.0 0.0 0.0 0.0 15 0.007108883660897364 0.0 0.0 0.0 0.0 16 0.007997494118509533 0.0 0.0 0.0 0.0 17 0.009330409804927789 0.0 0.0 0.0 0.0 18 0.010441172876943003 0.0 0.0 0.0 0.0 19 0.012662699020973429 0.0 0.0 0.0 0.0 20 0.01532853039380994 0.0 0.0 0.0 0.0 21 0.015994988237019066 0.0 0.0 0.0 0.0 22 0.01777220915224341 0.0 0.0 0.0 0.0 23 0.020215887910676876 0.0 0.0 0.0 0.0 24 0.021104498368289048 0.0 0.0 0.0 0.0 25 0.022215261440304262 0.0 0.0 0.0 0.0 26 0.02377032974112556 0.0 0.0 0.0 0.0 27 0.025325398041946858 0.0 0.0 0.0 0.0 28 0.029546297715604665 0.0 0.0 0.0 0.0 29 0.03065706078761988 0.0 0.0 0.0 0.0 30 0.03265643431724726 0.0 0.0 0.0 0.0 31 0.03510011307568073 0.0 0.0 0.0 0.0 32 0.03687733399090507 0.0 0.0 0.0 2.221526144030426E-4 33 0.03843240229172637 0.0 0.0 0.0 2.221526144030426E-4 34 0.0406539284357568 0.0 0.0 0.0 2.221526144030426E-4 35 0.04554128595262373 0.0 0.0 0.0 2.221526144030426E-4 36 0.04842926993986329 0.0 0.0 0.0 2.221526144030426E-4 37 0.050650796083893716 0.0 0.0 0.0 2.221526144030426E-4 38 0.05353878007113327 0.0 0.0 0.0 2.221526144030426E-4 39 0.055982458829566734 0.0 0.0 0.0 2.221526144030426E-4 40 0.05887044281680629 0.0 0.0 0.0 2.221526144030426E-4 41 0.060869816346433674 0.0 0.0 0.0 2.221526144030426E-4 42 0.06331349510486714 0.0 0.0 0.0 2.221526144030426E-4 43 0.06486856340568845 0.0 0.0 0.0 2.221526144030426E-4 44 0.0673122421641219 0.0 0.0 0.0 2.221526144030426E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATTC 15 0.0080148475 50.6393 48 TCGGTTA 15 0.008264593 50.24776 47 TTCGTAA 15 0.008271917 50.2365 43 TAGTTAG 15 0.008271917 50.2365 45 TCGTAAC 15 0.008271917 50.2365 44 GTAACGT 15 0.008271917 50.2365 46 TCACGTT 15 0.008426844 50.00123 31 GTCGACA 20 4.194457E-4 49.967796 29 GACGAGT 15 0.008449152 49.967796 29 TATCGTG 15 0.008456597 49.956665 28 GTATCGT 15 0.008460322 49.9511 27 CCGTTGA 20 4.2501016E-4 49.83452 20 TTAGGTC 15 0.008553835 49.812374 13 ATTGTCG 20 4.2594326E-4 49.812374 13 CACCTAA 20 4.2594326E-4 49.812374 14 TCGGATA 20 4.2594326E-4 49.812374 5 TGATTCG 20 4.2594326E-4 49.812374 15 GTCGGTA 45 1.4188117E-10 49.812374 4 AGGGTCG 45270 0.0 49.212692 1 GGGTCGG 45180 0.0 49.079193 2 >>END_MODULE