FastQCFastQC Report
Fri 17 Jun 2016
SRR1526686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45424
Sequences flagged as poor quality0
Sequence length64
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8331.8338323353293413No Hit
GGGTTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3720.818950334624868No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGTGGTATCAA3390.7463015146178231No Hit
GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.6934660091581544No Hit
GGGTTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.3764529764001409No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGGGGTATCAA1490.32802042972877776No Hit
GGGTTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.3148115533638605No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGTGGGATCAA1140.2509686509334273No Hit
GGGTTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.1739168721380768No Hit
GGGTTTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.16951391334977106No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGGGGGATCAA750.1651109545614653No Hit
GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.15190207819654808No Hit
GGGTTTGGGAGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.14529764001408949No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.1430961606199366No Hit
GGGTTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG620.13649172243747798No Hit
GGGTTTGGGGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.12548432546671365No Hit
GGGTTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.11887988728425503No Hit
GGGTTTGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.10787249031349065No Hit
GGGTTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG470.10346953152518493No Hit
GGGTTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.10126805213103206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTC150.00445248758.6644356
CTGGCTC150.00445248758.6644356
TGCTTTT150.00445248758.6644357
AGGGGGA258.355975E-658.2086654
CGGAGAC406.712071E-1058.2086652
TCGGAGA351.549779E-858.20865651
AGACACG351.549779E-858.20865655
GTGGTTT150.004592356358.20865255
CTGAGTC351.5634214E-858.14412346
GGGTAGC201.9653799E-458.14412347
AGTCGGA351.5634214E-858.14412349
TGAGTCG351.5634214E-858.14412347
GTCGGAG351.5634214E-858.14412350
TGGGTAG150.004612601458.1441246
GTACTGA406.85759E-1058.0797443
ACGTACT406.85759E-1058.0797441
TACGTAC406.85759E-1058.0797440
CGTACTG406.85759E-1058.0797442
ACTGAGT351.5772457E-858.07973545
GGATTGG150.00463291358.07973542
Produced by FastQC (version 0.11.5)