FastQCFastQC Report
Fri 17 Jun 2016
SRR1526685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48719
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13702.812044582195858No Hit
GGGTTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5491.1268704201646174No Hit
GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.6342494714587738No Hit
GGGTTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1770.36330794967055974No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC1570.32225620394507276Illumina Single End Adapter 2 (96% over 31bp)
GGGTTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.2935199819372319No Hit
GGGTTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.28325704550586017No Hit
GGGTTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1210.2483630616391962No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGTGGTATCAA1160.23810012520782448No Hit
GGGTTTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.21552166505880663No Hit
GGGTTTGGGAGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.21141649048625794No Hit
GGGTTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.21141649048625794No Hit
GGGTTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG890.18268026847841706No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA810.16625957018822224Illumina Single End Adapter 2 (97% over 34bp)
GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.15804922104312485No Hit
GGGTTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.14573369732547875No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.14368111003920442No Hit
GGGTTTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.14368111003920442No Hit
GGGTTTGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.14162852275293009No Hit
GGGTTTGGGGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.14162852275293009No Hit
GGGTTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG670.13752334818038137No Hit
GGGTTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.12315523717646093No Hit
GGGTTTGGGAGTGCAGTGCTGGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.12110264989018658No Hit
GGGTTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.11289230074508919No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA500.10262936431371743Illumina Single End Adapter 2 (100% over 34bp)
GGGTTTGGGATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.1005767770274431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAGT303.648911E-758.0865145
GTACTGA303.648911E-758.0865143
AGGCGGC150.00463287658.0865146
CGTACTG303.648911E-758.0865142
TACTGAG303.648911E-758.0865144
AAACTCT406.9303496E-1058.0267534
GGCAAAC150.004651822658.0267536
TGCGATC258.522529E-658.0267532
AACTCTA406.9303496E-1058.0267535
CGGGCAA150.004651822658.0267534
TCCGGGC150.004651822658.0267532
TCAAACT406.9303496E-1058.0267532
GCAAACT150.004651822658.0267537
CCGGGCA150.004651822658.0267533
ACGGCGT201.9866551E-458.0267538
CAAACTG150.004651822658.0267538
CAAACTC406.9303496E-1058.0267533
AACGGCG201.9866551E-458.0267537
ACGTACT351.5919795E-858.02674541
TCTACGT351.5919795E-858.02674538
Produced by FastQC (version 0.11.5)