FastQCFastQC Report
Fri 17 Jun 2016
SRR1526684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48254
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12772.6464127326232023No Hit
GGGTTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5221.0817756040949973No Hit
GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.6175653831806689No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC1550.321216893936254Illumina Single End Adapter 2 (96% over 31bp)
GGGTTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.30878269159033445No Hit
GGGTTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1390.28805902101380193No Hit
GGGTTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.2611182492643097No Hit
GGGTTTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.22588800928420444No Hit
GGGTTTGGGAGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.20723670576532513No Hit
GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA970.2010196045923654No Hit
GGGTTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.19894723753471213No Hit
GGGTTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG890.18444066813113938No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.17615119990052638No Hit
GGGTTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.16993409872756662No Hit
GGGTTTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.15957226343930037No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGTGGTATCAA750.15542752932399387No Hit
GGGTTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG710.14713806109338085No Hit
GGGTTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG710.14713806109338085No Hit
GGGTTTGGGGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.1409209599204211No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA660.1367762258051146Illumina Single End Adapter 2 (97% over 34bp)
GGGTTTGGGAGTGCAGTGCTGGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.1326314916898081No Hit
GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.1201972893438886No Hit
GGGTTTCTGCTTCGATTTCCTTTTTCCTGTAGAGAAACGTCAAAGCTGAGAAAGATGGCTTCCC570.11812492228623535No Hit
GGGTTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT500.10361835288266256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAGT303.4516597E-758.5515845
CCAAGGC150.00448829258.5515845
TGCCAAG150.00450705658.4900143
ACGTACT303.502355E-758.42857441
TACGTAC303.502355E-758.42857440
CGTACTG303.502355E-758.42857442
GACTGCC150.00452587858.42857440
CTACGTA303.502355E-758.42857439
AGACTGC150.00452587858.42857439
CCATGAT303.579571E-758.2450326
GCCATGA303.579571E-758.2450325
CCATAAA150.004582695758.2450326
AGATCCA150.004582695758.2450322
TGAGCCA351.5448677E-858.24502622
GGAGACA351.5576006E-858.18409753
CGGAGAC351.5576006E-858.18409752
TCGGAGA351.5576006E-858.18409751
AGACACG351.5576006E-858.18409755
GTCGGAG351.5576006E-858.18409750
CTGAGTC351.5705155E-858.12330246
Produced by FastQC (version 0.11.5)