SRR1526683_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526683_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41212
Sequences flagged as poor quality	0
Sequence length	56
%GC	38

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	1438	3.4892749684557898	No Hit
GGGTTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	605	1.4680190235853634	No Hit
GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	275	0.6672813743569834	No Hit
GGGTTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG	143	0.34698631466563135	No Hit
GGGTTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	135	0.3275744928661555	No Hit
GGGTTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127	0.3081626710666796	No Hit
GGGTTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	114	0.27661846064253126	No Hit
GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	107	0.2596331165679899	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGT	106	0.25720663884305545	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG	104	0.2523536833931864	Illumina Single End Adapter 2 (95% over 23bp)
GGGTTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG	98	0.23779481704357952	No Hit
GGGTTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC	89	0.2159565175191692	No Hit
GGGTTTGGGAGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	89	0.2159565175191692	No Hit
GGGTTTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	87	0.2111035620693002	No Hit
GGGTTTGGGGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76	0.18441230709502085	No Hit
GGGTTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA	73	0.1771328739202174	No Hit
GGGTTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69	0.16742696302047946	No Hit
GGGTTTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	67	0.16257400757061052	No Hit
GGGTTTCTGCTTCGATTTCCTTTTTCCTGTAGAGAAACGTCAAAGCTGAGAAAGAT	63	0.15286809667087256	No Hit
GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63	0.15286809667087256	No Hit
GGGTTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60	0.1455886634960691	No Hit
GGGTTTGGGAGTGCAGTGCTGGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	59	0.14316218577113463	No Hit
GGGTTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	57	0.13830923032126563	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT	56	0.13588275259633117	Illumina Single End Adapter 2 (100% over 29bp)
GGGTTTGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA	54	0.1310297971464622	No Hit
GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.12860331942152772	No Hit
GGGTTTGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52	0.1261768416965932	No Hit
GGGTTTGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49	0.11889740852178976	No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG	47	0.11404445307192079	Illumina Single End Adapter 2 (96% over 30bp)
GGGTTTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.11161797534698632	No Hit
GGGTTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	42	0.10191206444724837	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGAAG	15	0.007996931	50.584465	50
AGTCGGA	40	2.3228495E-9	50.58446	49
GTCGGAG	40	2.3228495E-9	50.58446	50
TTCATGA	15	0.0082337875	50.212975	44
ACTGAGT	40	2.4792826E-9	50.212975	45
TCATGAT	15	0.0082337875	50.212975	45
TACTGAG	40	2.4792826E-9	50.212975	44
CAGCCAC	15	0.008273765	50.151592	34
AGCCACA	15	0.008273765	50.151592	35
ACGTAGA	30	1.0019285E-6	50.151592	41
GTTCATG	15	0.008273765	50.151592	43
AGTACGT	30	1.0019285E-6	50.151592	38
TACGTAG	30	1.0019285E-6	50.151592	40
GCCACAT	15	0.008273765	50.151592	36
GTACGTA	30	1.0019285E-6	50.151592	39
GTTGCCG	15	0.008273765	50.151592	37
TTGCCGT	15	0.008273765	50.151592	38
AAACTCT	40	2.5065674E-9	50.15159	34
GTACTGA	40	2.5065674E-9	50.15159	43
CAGTACG	35	5.0024028E-8	50.15159	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content