##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526683_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41212 Sequences flagged as poor quality 0 Sequence length 56 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.00778899349704 38.0 38.0 39.0 35.0 40.0 2 37.66233135979812 38.0 38.0 39.0 35.0 40.0 3 37.67414830631855 38.0 38.0 39.0 35.0 40.0 4 35.25499854411336 36.0 34.0 39.0 29.0 39.0 5 37.83701349121615 38.0 38.0 39.0 35.0 40.0 6 37.80219353586334 38.0 38.0 39.0 35.0 40.0 7 37.59201203532952 38.0 38.0 39.0 35.0 40.0 8 37.64095409104144 38.0 38.0 39.0 35.0 40.0 9 37.53069494322042 38.0 38.0 39.0 35.0 40.0 10 36.448364554013395 38.0 36.0 39.0 32.0 40.0 11 37.18727555081044 39.0 37.0 39.0 33.0 40.0 12 37.09443851305445 39.0 37.0 39.0 33.0 40.0 13 36.780209647675434 39.0 36.0 39.0 32.0 40.0 14 36.993763952246915 39.0 36.0 39.0 33.0 40.0 15 36.99381248180142 39.0 37.0 39.0 33.0 40.0 16 36.61115694457925 39.0 36.0 39.0 32.0 40.0 17 36.859434145394545 39.0 37.0 39.0 33.0 40.0 18 36.65946811608269 39.0 36.0 39.0 32.0 40.0 19 36.20566825196545 38.0 36.0 39.0 31.0 40.0 20 36.48012714743279 39.0 36.0 39.0 31.0 40.0 21 36.335800252353685 38.0 36.0 39.0 32.0 40.0 22 36.1963020479472 38.0 36.0 39.0 31.0 40.0 23 35.948558672231385 38.0 36.0 39.0 30.0 40.0 24 35.818814908279144 38.0 35.0 39.0 30.0 40.0 25 35.73522275065515 38.0 35.0 39.0 30.0 40.0 26 35.53596039988353 38.0 35.0 39.0 30.0 40.0 27 35.4890808502378 38.0 35.0 39.0 29.0 40.0 28 35.11923711540328 38.0 35.0 39.0 29.0 40.0 29 35.242016888284965 38.0 35.0 39.0 29.0 40.0 30 34.770236824225954 38.0 35.0 39.0 27.0 40.0 31 34.56898476171989 38.0 35.0 39.0 25.0 40.0 32 34.09591866446666 38.0 34.0 39.0 23.0 40.0 33 34.39085703193245 38.0 35.0 39.0 23.0 40.0 34 34.38976511695623 39.0 35.0 39.0 24.0 40.0 35 33.99024555954576 39.0 34.0 39.0 21.0 40.0 36 34.00606619431234 39.0 35.0 39.0 14.0 40.0 37 33.90029603028244 39.0 35.0 39.0 14.0 40.0 38 33.75169853440745 39.0 35.0 39.0 11.0 40.0 39 33.715665340192174 39.0 35.0 39.0 9.0 40.0 40 33.5166213724158 39.0 34.0 39.0 4.0 40.0 41 33.40847326021547 39.0 35.0 39.0 4.0 40.0 42 33.32803552363389 39.0 34.0 39.0 4.0 40.0 43 33.207172668154904 39.0 34.0 39.0 4.0 40.0 44 32.95508589731146 39.0 34.0 39.0 4.0 40.0 45 32.796782490536735 39.0 34.0 39.0 4.0 40.0 46 32.368606231194796 39.0 33.0 39.0 4.0 40.0 47 32.14592837037756 39.0 33.0 39.0 4.0 40.0 48 31.954600601766476 39.0 32.0 39.0 4.0 40.0 49 31.632849655440165 38.0 32.0 39.0 4.0 40.0 50 31.29852955449869 38.0 32.0 39.0 4.0 40.0 51 30.61666504901485 38.0 31.0 39.0 4.0 40.0 52 30.39699602057653 37.0 31.0 39.0 4.0 40.0 53 30.02967582257595 37.0 30.0 39.0 4.0 40.0 54 29.39221585945841 37.0 27.0 39.0 4.0 40.0 55 28.941206444724838 36.0 23.0 39.0 4.0 40.0 56 17.109409880617296 21.0 4.0 25.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.048379774419125 1 2 -1.8910436279541258 1 3 -2.1366827530972117 1 4 -2.7951765582919066 1 5 -2.0360418078007996 1 6 -2.1122450019018757 1 7 -2.5249841139207945 1 8 -2.4115803025044613 1 9 -2.330971253126755 1 10 -2.844009716672815 1 11 -2.046869781978586 1 12 -1.8159167455392193 1 13 -2.224286408304174 1 14 -2.554276323728864 1 15 -2.641289009243927 1 16 -1.4277845695919424 1 17 -1.8450963007112122 1 18 -2.302500587111446 1 19 -1.7727867718402806 1 20 -2.0162923218343707 1 21 -1.7115602317061658 1 22 -2.6602857422251276 1 23 -3.198454402577241 1 24 -2.5177133731973242 1 25 -4.354040927817817 1 26 -4.031456537171216 1 27 -4.341341634322273 1 28 -3.2298610095420024 1 29 -3.5230903105983806 1 30 -3.2775787726551506 1 31 -2.7453625572139195 1 32 -2.7732530688021555 1 33 -2.7921730958588853 1 34 -2.882935654980397 1 35 -3.566376140759992 1 36 -2.438487439216818 1 37 -1.4441185139201451 1 38 -2.0477226744553647 1 39 -1.9305916196788253 1 40 -2.7027116969347382 1 41 -1.88653560334512 1 42 -2.1133420246464993 1 43 -2.1703410581295763 1 44 -2.2605645780820467 1 45 -1.831820770595133 1 46 -1.308683879038707 1 47 -1.183035785860632 1 48 -2.0977991513628638 1 49 -3.3386256360273627 1 50 -2.265474613890021 1 51 -1.6041104261411974 1 52 -1.7093232514301953 1 53 -2.1124090035157828 1 54 -2.361430414395077 1 55 -1.6851104153920389 1 56 -0.8253431981445765 2 1 0.042529316489968494 2 2 0.08622909931860079 2 3 -3.191167335785394E-4 2 4 -1.1133583764737267 2 5 0.28214001038102055 2 6 -0.08951772917460232 2 7 -0.36589320482988796 2 8 -0.025216666140821076 2 9 -0.24006216221766863 2 10 -0.7531006257637287 2 11 -1.5014152365240392 2 12 -0.9522803819028525 2 13 -0.9515591355769004 2 14 -1.236094505547051 2 15 -1.3685617365166536 2 16 -1.1323300241373957 2 17 -1.3223690279839388 2 18 -1.688864223475079 2 19 -0.43187768093118706 2 20 -0.7435650491070973 2 21 -0.8024693226152522 2 22 -0.8421039240433075 2 23 -1.016636220759061 2 24 -0.6313497368336911 2 25 -0.21767729145418002 2 26 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0.19245962793605997 3 11 -0.022556885572669216 3 12 -0.04424655526437249 3 13 0.5072146487782803 3 14 0.33737272489692316 3 15 0.2730873121091335 3 16 -0.006008671071853655 3 17 -0.3096840385547637 3 18 -0.2929868450395645 3 19 0.42277348185950814 3 20 0.27229118767937166 3 21 -0.23058771584992854 3 22 0.6304119321934749 3 23 0.42416716190479065 3 24 0.6059652737371337 3 25 0.677143004528908 3 26 0.19000223661313953 3 27 0.01013828111113213 3 28 0.6728874048343201 3 29 0.7718356936299458 3 30 1.2440914176196927 3 31 -0.008576087869315074 3 32 0.3250555992739521 3 33 0.05613557221722232 3 34 0.3353518714466617 3 35 -0.0024226523878994044 3 36 0.47641742336246296 3 37 0.7012303232891597 3 38 0.4295500528173619 3 39 0.3627487608708577 3 40 -0.5034516546514354 3 41 0.4242466376696825 3 42 0.4220702375099492 3 43 0.38515577062306505 3 44 0.4952493754063241 3 45 0.6295957198911282 3 46 0.17757404907968777 3 47 0.2540678082197019 3 48 0.49998097548703413 3 49 0.43198747179504693 3 50 0.2672949421353472 3 51 0.019568220793264146 3 52 0.3731291798594434 3 53 0.20682989711846744 3 54 -0.5485340084754142 3 55 0.1568557579694847 3 56 -0.6065271304913011 4 1 0.01980204376269512 4 2 0.3833466159483123 4 3 -0.3196095823655014 4 4 -0.6139127001987816 4 5 0.018836240979688057 4 6 0.1782206300271767 4 7 0.1352154426202219 4 8 0.2846502961651609 4 9 0.14075823689541522 4 10 0.3439060261209761 4 11 0.9076756725668673 4 12 0.7583626336182121 4 13 0.5650705761747616 4 14 0.738960926825456 4 15 0.5039438067205921 4 16 0.02121764770296153 4 17 0.20534715826882888 4 18 -0.028664666934062666 4 19 0.11080524589807794 4 20 0.6477875007820373 4 21 -0.12508573059751882 4 22 0.5226410870431621 4 23 1.345891495427196 4 24 0.9445926134989051 4 25 0.1587085178584573 4 26 -1.5541838098984897 4 27 -0.5525389735683888 4 28 -1.4626769740652819 4 29 -0.5463719070507125 4 30 -0.8557384178879701 4 31 -0.9881563487882019 4 32 -0.3175989668065924 4 33 -0.5133482621560006 4 34 -0.3912505108562314 4 35 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7 29 -0.03824182574989976 7 30 0.10012685505047614 7 31 -0.6176569295082928 7 32 -1.3100496055986923 7 33 -0.7694458231316119 7 34 -0.4835850056297488 7 35 -1.3878047121885615 7 36 -0.588920339649718 7 37 -0.9798327996344298 7 38 -0.713307090039784 7 39 -0.8775613166485243 7 40 0.014820770597729904 7 41 0.011732794923275947 7 42 -1.2074978688023457 7 43 -0.7709904087789283 7 44 0.3584830409655737 7 45 -0.7679679567423179 7 46 -0.5034890738439017 7 47 -0.6451569979818466 7 48 -1.9452017487654594 7 49 -0.33213212953385707 7 50 -0.38885123726664617 7 51 0.3158029937722233 7 52 0.29284125073430545 7 53 0.7079373168305381 7 54 0.8268812739166123 7 55 1.919867939586318 7 56 1.217946845145466 8 1 -0.030197956237302037 8 2 -0.16604362795412442 8 3 0.21331724690278975 8 4 1.0775507144353682 8 5 0.43895819219920185 8 6 0.6013913617344926 8 7 0.45228861335193216 8 8 0.08160151567735596 8 9 -0.36960761676311904 8 10 1.4378084651453662 8 11 0.9826756725668702 8 12 0.4522650726426036 8 13 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120 48 1.9976553940916801 120 49 3.375010727609002 120 50 2.5004344770190663 120 51 3.277707755676989 120 52 3.5952222031152594 120 53 2.385318269211492 120 54 2.05902413105947 120 55 0.11034413006250787 120 56 0.4905658927645149 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 64.0 5 20.0 6 32.0 7 31.0 8 111.0 9 132.0 10 96.0 11 146.0 12 146.0 13 129.0 14 195.0 15 204.0 16 208.0 17 248.0 18 272.0 19 294.0 20 310.0 21 412.0 22 399.0 23 374.0 24 463.0 25 343.0 26 352.0 27 398.0 28 518.0 29 611.0 30 823.0 31 1102.0 32 1277.0 33 1682.0 34 2135.0 35 2661.0 36 3555.0 37 6751.0 38 9148.0 39 5570.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.01242356595166 0.3081626710666796 0.4440454236630108 0.23536833931864504 8 98.65776699029126 0.691747572815534 0.5145631067961165 0.13592233009708737 9 96.14675337280404 1.9775793458216055 0.8662525478016112 1.0094147335727457 10 23.607201785887604 51.75676987285257 9.931573328156848 14.70445501310298 11 20.99145879840823 44.91167621081239 19.19343880423178 14.903426186547609 12 52.60118412112976 18.341745122779773 16.856740755119866 12.200330000970592 13 24.170144618072406 15.160632825390664 27.50897796758226 33.160244588954676 14 27.346403960011646 34.36135106279725 19.98932349801029 18.30292147918082 15 43.174318159759295 20.95748811025915 19.72483742599243 16.14335630398913 16 22.102785596428227 24.094923808599436 32.218771231680094 21.583519363292243 17 35.19363292244977 22.736096282636126 18.429098320877415 23.64117247403669 18 33.25487722022712 26.29331262739008 20.955061632534214 19.496748519848587 19 20.773075803164126 25.009705910899736 32.80355236338931 21.413665922546834 20 21.91594681160827 34.565175191691736 18.7518198582937 24.767058138406288 21 23.62661360768708 22.449771911093855 32.18965349898088 21.73396098223818 22 26.49956323400951 23.597495874987867 20.05483839658352 29.8481024944191 23 28.693099097350288 33.05347956905755 21.974182277006697 16.27923905658546 24 22.02620723125455 37.37442368357195 24.743994176170833 15.85537490900267 25 21.894568224843454 20.098538905878353 31.16353575069171 26.843357118586475 26 30.34188034188034 19.502719502719504 31.179584304584306 18.97581585081585 27 19.550807332766784 19.147747966492656 31.77370401845332 29.52774068228724 28 19.200777359407265 30.370460342523987 34.23053564921657 16.19822664885218 29 28.19253676917467 20.622341072079735 35.390786434909444 15.79433572383615 30 15.737609187571474 33.09326260979586 38.52648482931458 12.642643373318085 31 23.136653522223845 25.035295263132273 40.835402365999705 10.992648848644174 32 11.514871245679794 24.110402570218568 52.91340115854549 11.46132502555615 33 15.48555152518441 25.00669474401733 44.83044039243372 14.677313338364534 34 14.05230349663972 26.1712282068764 46.81503847277686 12.961429823707022 35 11.003386032008965 33.53876884850552 47.05366495335071 8.404180166134807 36 12.716903879898616 26.36478845778904 48.125852992786115 12.792454669526224 37 9.978551233304085 25.463098371843618 54.82840986643268 9.729940528419617 38 12.478361575033524 25.456540290137752 53.85103011093503 8.214068023893697 39 10.46418030587604 25.421372295533818 52.986803912481406 11.12764348610874 40 10.435737824972538 25.306969364091298 55.91846698401074 8.338825826925424 41 11.36913046663248 26.740409737992334 53.05838400117208 8.83207579420311 42 12.386116606824455 25.270511223467917 52.41445006228476 9.928922107422878 43 14.167379352474038 24.979841172877215 52.63042150274894 8.222357971899816 44 9.19175136825645 26.790949960906957 56.01055512118843 8.006743549648162 45 10.322154868987095 27.55915134923739 54.78099335158389 7.337700430191631 46 9.674973709310573 26.858079190002197 54.1832766758786 9.283670424808628 47 11.822696249908265 28.166051028645516 52.65784388072115 7.353408840725066 48 13.272798472759312 27.710607469773365 51.245778060600124 7.770815996867199 49 12.57767774135147 29.153006801389637 50.99818955815433 7.271125899104565 50 9.602859801185055 30.326624553156066 52.31379462318202 7.756721022476862 51 9.236643089649771 31.46185662889449 51.60674514794782 7.694755133507917 52 11.83714439602409 29.026098026734566 50.26195955540322 8.874798021838123 53 10.28353165858675 29.663091915185348 50.02448459918711 10.028891827040791 54 10.437012922955509 31.047153990332443 49.56841274538818 8.947420341323863 55 11.544679600235156 30.592298647854204 48.49598275524201 9.367038996668626 56 12.052412441831986 30.34778349253 48.679892236100905 8.919911829537105 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 4.0 5 8.0 6 5.5 7 3.0 8 8.0 9 13.0 10 54.0 11 95.0 12 94.0 13 101.5 14 110.0 15 145.5 16 181.0 17 394.0 18 607.0 19 803.0 20 999.0 21 996.5 22 1276.0 23 1558.0 24 1909.0 25 2260.0 26 2523.0 27 2786.0 28 3049.0 29 3401.5 30 3491.0 31 3612.5 32 3734.0 33 2980.0 34 2226.0 35 2444.0 36 2662.0 37 1774.5 38 758.5 39 630.0 40 618.0 41 606.0 42 633.5 43 661.0 44 677.5 45 694.0 46 803.5 47 828.0 48 743.0 49 770.0 50 797.0 51 879.0 52 961.0 53 1182.0 54 1596.0 55 1789.0 56 1657.0 57 1525.0 58 1374.5 59 1224.0 60 1133.0 61 1042.0 62 910.5 63 645.5 64 512.0 65 422.0 66 332.0 67 267.0 68 202.0 69 175.5 70 149.0 71 116.0 72 75.0 73 67.0 74 50.5 75 34.0 76 27.5 77 21.0 78 16.5 79 9.0 80 6.0 81 3.0 82 0.0 83 2.5 84 5.0 85 3.0 86 1.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.029117732699213823 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.00485295544986897 25 0.02426477724934485 26 0.06794137629816559 27 0.0655148985732311 28 0.11404445307192079 29 0.18683878481995536 30 0.27419198291759683 31 0.31544210424148306 32 0.3057361933417451 33 0.3275744928661555 34 0.3494127923905659 35 0.39066291371445216 36 0.4367659904882073 37 0.4464719013879453 38 0.4780161118120936 39 0.5216927108609143 40 0.5993399980588179 41 0.6284577307580317 42 0.6575754634572455 43 0.6963991070561972 44 0.6891196738813937 45 0.7279433174803456 46 0.7837523051538388 47 0.8080170824031835 48 0.8589731146268077 49 0.820149471027856 50 0.8977967582257594 51 0.8565466369018733 52 0.8880908473260216 53 0.8977967582257594 54 1.6111812093564981 55 0.9414733572745801 56 0.9269144909249732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 41212.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.11258856643696 #Duplication Level Percentage of deduplicated Percentage of total 1 75.18601681856205 41.4369601087062 2 13.071809096112357 14.408424730660974 3 4.843041430018051 8.0073764922838 4 2.2410073526174434 4.940308647966612 5 1.3780654250869546 3.797437639522469 6 0.849733632721349 2.809861205474134 7 0.5943732664113064 2.2930214500630886 8 0.4138599040197244 1.8247112491507327 9 0.29938801567384316 1.485004367659905 >10 1.0038304054946507 8.516936814520044 >50 0.07484700391846079 2.826846549548675 >100 0.03522211949104037 2.6958167524022127 >500 0.004402764936380046 1.4680190235853634 >1k 0.004402764936380046 3.4892749684557898 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1438 3.4892749684557898 No Hit GGGTTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 605 1.4680190235853634 No Hit GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 275 0.6672813743569834 No Hit GGGTTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 143 0.34698631466563135 No Hit GGGTTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 135 0.3275744928661555 No Hit GGGTTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 0.3081626710666796 No Hit GGGTTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 114 0.27661846064253126 No Hit GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 107 0.2596331165679899 No Hit GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGGTTTGGGAAGCAGT 106 0.25720663884305545 No Hit GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 104 0.2523536833931864 Illumina Single End Adapter 2 (95% over 23bp) GGGTTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 98 0.23779481704357952 No Hit GGGTTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 89 0.2159565175191692 No Hit GGGTTTGGGAGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 89 0.2159565175191692 No Hit GGGTTTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 87 0.2111035620693002 No Hit GGGTTTGGGGTGCAGTGCTGGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.18441230709502085 No Hit GGGTTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 73 0.1771328739202174 No Hit GGGTTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.16742696302047946 No Hit GGGTTTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67 0.16257400757061052 No Hit GGGTTTCTGCTTCGATTTCCTTTTTCCTGTAGAGAAACGTCAAAGCTGAGAAAGAT 63 0.15286809667087256 No Hit GGGTTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 63 0.15286809667087256 No Hit GGGTTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60 0.1455886634960691 No Hit GGGTTTGGGAGTGCAGTGCTGGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.14316218577113463 No Hit GGGTTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 57 0.13830923032126563 No Hit GGGTTTGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTCTTCT 56 0.13588275259633117 Illumina Single End Adapter 2 (100% over 29bp) GGGTTTGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.1310297971464622 No Hit GGGTTTGGGGAGTGCAGTGCTGGGTTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.12860331942152772 No Hit GGGTTTGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52 0.1261768416965932 No Hit GGGTTTGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49 0.11889740852178976 No Hit GGGTTTGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 47 0.11404445307192079 Illumina Single End Adapter 2 (96% over 30bp) GGGTTTGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46 0.11161797534698632 No Hit GGGTTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.10191206444724837 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.00485295544986897 0.0 0.0 0.0 0.0 9 0.012132388624672426 0.0 0.0 0.0 0.0 10 0.160147529845676 0.0 0.0 0.0 0.0 11 0.2790449383674658 0.0 0.0 0.0 0.0 12 0.28875084926720374 0.0 0.0 0.0 0.0 13 0.2911773269921382 0.0 0.0 0.0 0.0 14 0.34941279239056583 0.0 0.0 0.0 0.0 15 0.35669222556536934 0.0 0.0 0.0 0.0 16 0.3785305250897797 0.0 0.0 0.0 0.0 17 0.3809570028147142 0.0 0.0 0.0 0.0 18 0.38338348053964866 0.0 0.0 0.0 0.0 19 0.39794234688925556 0.0 0.0 0.0 0.0 20 0.4343395127632728 0.0 0.0 0.0 0.0 21 0.4610307677375522 0.0 0.0 0.0 0.0 22 0.49500145588663497 0.0 0.0 0.0 0.0 23 0.5459574881102591 0.0 0.0 0.0 0.0 24 0.5896340871590798 0.0 0.0 0.0 0.0 25 0.5969135203338833 0.0 0.0 0.0 0.0 26 0.5969135203338833 0.0 0.0 0.0 0.0 27 0.6138988644084248 0.0 0.0 0.0 0.0 28 0.7788993497039697 0.0 0.0 0.0 0.0 29 0.7934582160535766 0.0 0.0 0.0 0.0 30 0.8613995923517422 0.0 0.0 0.0 0.0 31 0.8711055032514802 0.0 0.0 0.0 0.0 32 0.890517325050956 0.0 0.0 0.0 0.0 33 0.9099291468504319 0.0 0.0 0.0 0.0 34 0.9220615354751044 0.0 0.0 0.0 0.0 35 1.0094147335727457 0.0 0.0 0.0 0.0 36 1.0239735999223527 0.0 0.0 0.0 0.0 37 1.040958943996894 0.0 0.0 0.0 0.0 38 1.065223721246239 0.0 0.0 0.0 0.0 39 1.0846355430457149 0.0 0.0 0.0 0.0 40 1.1064738425701253 0.0 0.0 0.0 0.0 41 1.1234591866446666 0.0 0.0 0.0 0.0 42 1.145297486169077 0.0 0.0 0.0 0.0 43 1.1719887411433563 0.0 0.0 0.0 0.0 44 1.1889740852178976 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGAAG 15 0.007996931 50.584465 50 AGTCGGA 40 2.3228495E-9 50.58446 49 GTCGGAG 40 2.3228495E-9 50.58446 50 TTCATGA 15 0.0082337875 50.212975 44 ACTGAGT 40 2.4792826E-9 50.212975 45 TCATGAT 15 0.0082337875 50.212975 45 TACTGAG 40 2.4792826E-9 50.212975 44 CAGCCAC 15 0.008273765 50.151592 34 AGCCACA 15 0.008273765 50.151592 35 ACGTAGA 30 1.0019285E-6 50.151592 41 GTTCATG 15 0.008273765 50.151592 43 AGTACGT 30 1.0019285E-6 50.151592 38 TACGTAG 30 1.0019285E-6 50.151592 40 GCCACAT 15 0.008273765 50.151592 36 GTACGTA 30 1.0019285E-6 50.151592 39 GTTGCCG 15 0.008273765 50.151592 37 TTGCCGT 15 0.008273765 50.151592 38 AAACTCT 40 2.5065674E-9 50.15159 34 GTACTGA 40 2.5065674E-9 50.15159 43 CAGTACG 35 5.0024028E-8 50.15159 37 >>END_MODULE