FastQCFastQC Report
Fri 17 Jun 2016
SRR1526682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438088
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT15260.34833184200434614No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC13420.30633114807983786No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.19927503150052045No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7690.17553550884753746No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC7560.1725680685159146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATTA201.9848396E-458.1420256
TCGGTAC201.9848396E-458.1420255
TTCGTAA202.0040658E-458.02876343
TCGTAAC150.004676099458.0287644
TGCGACA258.788837E-657.85639623
ACGATTG150.004733642657.8497816
TTCGTAC150.004740068757.82996420
TCGGGAT44600.055.7302975
ATCGGGG198100.055.339114
CGGGATT13200.055.278976
TCGGGAC31200.055.2535555
TCGGGAG60700.055.0768745
GATCGGG437850.054.928513
ATCGGGT26850.054.8938644
AGATCGG455650.054.8562552
GAGATCG461100.054.6311381
TCGGGGG97850.054.5174265
ATCGGGA180050.054.4284254
TCGGGGC35850.054.3307575
ATCGGGC33650.053.9087944
Produced by FastQC (version 0.11.5)