FastQCFastQC Report
Fri 17 Jun 2016
SRR1526681_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526681_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences613687
Sequences flagged as poor quality0
Sequence length64
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT45910.748101230757699No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC36370.5926473919115118No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC26820.43703060354871454No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT22000.35848893654256975No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC19690.32084759820559994No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12960.21118257352689562No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11910.19407287428281844No Hit
GAGATCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG11600.1890214392679004No Hit
GAGATCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT10790.17582252842246943No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA9740.15871282917839225No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC9670.1575721825621204No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC9330.15203189899737163No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC8820.14372147365024843No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC7640.12449343069023786No Hit
GAGATCGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGTC6430.1047765391803965No Hit
GAGATCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG6160.10037690223191954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAA150.004617412658.21697653
TCGTAAT150.004617412658.21697654
CGTAATT150.004617412658.21697655
ATCGTCG150.00463393258.16449452
AACGATA258.545387E-658.13590244
CGTTAAC150.004683753558.00760330
ATATCGC150.004743146757.8232714
ATGCGAA150.004743146757.8232710
CGGGATA6250.056.0140046
ATCGGAT3000.055.9368484
TCGGGAT62900.055.6117975
TCGGGAC39750.055.6093065
CGGGACT15400.055.423326
ATCGGGG284900.054.910474
GATCGGG614900.054.8825453
TCGGGGG130350.054.8470155
ATCGGGA246150.054.8213774
AGATCGG640650.054.7901952
GAGATCG645250.054.740561
TCGGGGC53500.054.458665
Produced by FastQC (version 0.11.5)