FastQCFastQC Report
Fri 17 Jun 2016
SRR1526680_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526680_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611968
Sequences flagged as poor quality0
Sequence length64
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT41410.6766693683329847No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC33240.5431656557205605No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC25050.4093351286341769No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT19050.31129078644635017No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC18290.29887183643589205No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.21063192846684795No Hit
GAGATCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG10490.17141419159171722No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10430.17043374816983894No Hit
GAGATCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT9360.15294917381300982No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC9180.15000784354737504No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA9050.1478835494666388No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC9010.14722992051871994No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC8120.13268667642752563No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC7880.12876490274001254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTCG150.004660343358.08102456
CCTTACG202.026826E-457.90003634
ATATCGC150.00478604357.6918914
CGGGATA6500.056.925256
TCGGGAT58850.055.7025265
TCGGGAC42450.055.36325
ATCGGGA247700.055.060514
TCGGGGG133700.055.047215
GATCGGG611300.054.9017453
AGATCGG635900.054.878062
ATCGGGG284500.054.857934
TCGGGGC51800.054.8570065
GAGATCG641600.054.738011
CGGGATG16350.054.4554446
TCGGGAG93850.054.303325
CGGGATC19450.054.247736
CGGGATT17750.054.2318276
ATCGGGC45900.053.784054
ATCGGGT34350.053.7752534
CGGGGGG79900.053.4369966
Produced by FastQC (version 0.11.5)