FastQCFastQC Report
Fri 17 Jun 2016
SRR1526679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences558111
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC48800.8743780359104192No Hit
GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG46930.8408721562556553No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC33050.5921761083368721No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC22340.4002787975868599No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT14200.25442967438376957No Hit
GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG14140.2533546194215846No Hit
GAGATCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT13580.24332077310785846No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9320.16699187079272762No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC9060.1623332992899262No Hit
GAGATCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA8350.14961181557073772No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC7620.13653198019748758No Hit
GAGATCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA6890.12345214482423748No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC6530.11700181505112782No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC6370.11413500181863465No Hit
GAGATCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6300.11288077102941887No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT5780.10356362802381605No Hit
GAGATCGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCAT5700.10213022140756947No Hit
GAGATCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC5690.10195104558053865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTTG150.00796373150.72294648
CGACGTT150.00842769550.0015330
CAATTCG150.00845471649.96105226
CGGTAAG150.00857558449.7819486
TATTTCG150.0085877449.7641115
GATAACG150.0085877449.764119
ATAACGA150.0085877449.7641110
ATTTCGG150.0085877449.7641116
TCGGGAT61800.048.7347535
CGGGATA6400.048.6151856
ATCGGAT3150.048.2015724
TCGGGAC37800.048.201575
ATCGGGA230150.047.7595254
CGGGATT17200.047.755946
TCGGGAG83750.047.552915
GATCGGG556750.047.4526823
AGATCGG580300.047.3927962
CGGGATC18650.047.3796046
GAGATCG584200.047.3661961
ATCGGGG255400.047.1895454
Produced by FastQC (version 0.11.5)