##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526679_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 558111 Sequences flagged as poor quality 0 Sequence length 56 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.70802761457846 39.0 39.0 40.0 36.0 40.0 2 37.82681760438336 39.0 38.0 40.0 33.0 40.0 3 38.43652427563693 39.0 38.0 40.0 35.0 40.0 4 38.037340242353224 39.0 38.0 40.0 35.0 40.0 5 38.40814819991005 39.0 38.0 40.0 35.0 40.0 6 38.63424838428198 39.0 39.0 40.0 36.0 40.0 7 38.84698384371568 39.0 39.0 40.0 38.0 40.0 8 38.787062071881756 39.0 39.0 40.0 38.0 40.0 9 38.72061471642738 39.0 39.0 40.0 37.0 40.0 10 37.96674138298654 39.0 38.0 40.0 35.0 40.0 11 38.07807049135387 39.0 38.0 40.0 35.0 40.0 12 37.91072206066535 39.0 38.0 40.0 35.0 40.0 13 37.686642979622334 39.0 38.0 40.0 34.0 40.0 14 37.85099738224117 39.0 38.0 40.0 34.0 40.0 15 37.87671090517836 39.0 38.0 40.0 34.0 40.0 16 37.6961249643888 39.0 38.0 40.0 34.0 40.0 17 37.619785311524055 39.0 38.0 40.0 33.0 40.0 18 37.49723262935151 39.0 37.0 40.0 33.0 40.0 19 37.41702098686462 39.0 37.0 40.0 33.0 40.0 20 37.34774086158488 39.0 37.0 40.0 33.0 40.0 21 37.21926283481243 39.0 37.0 40.0 33.0 40.0 22 37.11404541390512 39.0 36.0 40.0 33.0 40.0 23 37.011447543589 39.0 36.0 40.0 33.0 40.0 24 36.8463907717282 39.0 36.0 40.0 32.0 40.0 25 36.67884703938822 39.0 36.0 40.0 32.0 40.0 26 36.275335909881726 38.0 36.0 40.0 31.0 40.0 27 36.07103425662637 38.0 35.0 40.0 30.0 40.0 28 35.8207381685722 38.0 35.0 40.0 30.0 40.0 29 35.66181637702894 38.0 35.0 40.0 29.0 40.0 30 35.376355241161704 38.0 35.0 40.0 29.0 40.0 31 34.67880224543146 38.0 34.0 39.0 27.0 40.0 32 34.5309481447239 38.0 33.0 39.0 26.0 40.0 33 34.31231063354781 38.0 33.0 39.0 26.0 40.0 34 34.41095230160309 38.0 33.0 39.0 26.0 40.0 35 34.13184653232063 38.0 33.0 39.0 25.0 40.0 36 33.72544888024067 38.0 33.0 40.0 22.0 40.0 37 33.458484064997826 38.0 33.0 39.0 20.0 40.0 38 33.26350851353942 37.0 33.0 39.0 19.0 40.0 39 33.0824800084571 37.0 33.0 39.0 17.0 40.0 40 32.80226872432186 37.0 32.0 39.0 14.0 40.0 41 32.40778268122291 37.0 32.0 39.0 4.0 40.0 42 32.18555806998966 36.0 32.0 39.0 4.0 40.0 43 32.004276926991224 36.0 31.0 39.0 4.0 40.0 44 31.75515981587892 36.0 31.0 39.0 4.0 40.0 45 31.392409395263666 36.0 31.0 39.0 4.0 40.0 46 30.689918313740456 36.0 30.0 39.0 4.0 40.0 47 30.38035802913757 35.0 29.0 39.0 4.0 40.0 48 30.109089410529446 35.0 29.0 39.0 4.0 40.0 49 29.844762063460493 35.0 29.0 39.0 4.0 40.0 50 29.421684933642233 35.0 28.0 39.0 4.0 40.0 51 28.534891804676846 35.0 26.0 38.0 4.0 40.0 52 28.242845957166228 34.0 25.0 38.0 4.0 40.0 53 27.89846105882163 34.0 23.0 38.0 4.0 40.0 54 27.537676197028908 34.0 19.0 38.0 4.0 40.0 55 27.190567826113444 34.0 4.0 38.0 4.0 40.0 56 16.048966961769253 20.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5166574757072695 1 2 -0.8656022951318292 1 3 -0.5847283692070562 1 4 -1.0558701581000491 1 5 -1.4328472551096212 1 6 -0.8129232030033009 1 7 -0.6339789603251091 1 8 -0.7509663956809405 1 9 -0.7238064407216669 1 10 -0.557523273372091 1 11 -0.517217591143897 1 12 -0.8914018469314229 1 13 -0.4034177933415606 1 14 -0.6868233346518977 1 15 -0.8378950750873386 1 16 -0.35218757624551245 1 17 -0.5687405951978235 1 18 -0.35502165174793276 1 19 -0.6069223168796753 1 20 -0.4989952559956947 1 21 -0.18852375271944055 1 22 -0.3363368417725283 1 23 -0.3198482887554732 1 24 -0.47045331957409786 1 25 -1.3186380555444401 1 26 -0.29609445986436356 1 27 -0.9432949101751902 1 28 -0.07885548713667134 1 29 -0.3662720725505437 1 30 -0.26248537143798245 1 31 -0.29960600983917374 1 32 -0.5376463426067417 1 33 -0.3394120878092721 1 34 -0.5790320934279123 1 35 -0.9446609987127133 1 36 0.11493371671206631 1 37 -0.15848039346848708 1 38 0.3404940909691021 1 39 0.15253491852251244 1 40 0.0903148213872953 1 41 0.23190314577459503 1 42 0.3711558796119121 1 43 0.45522824282280183 1 44 0.3541731434389632 1 45 0.3740013307689658 1 46 0.7749483195206217 1 47 1.0303135516490798 1 48 0.6240165808607756 1 49 1.069240063107145 1 50 1.131216536793353 1 51 1.7441324851230924 1 52 2.012933985488857 1 53 1.6917219464902118 1 54 2.1072933540016585 1 55 1.6772926889927433 1 56 1.6106225205059843 2 1 -0.38629979836515815 2 2 -0.8441566201842079 2 3 -0.5389711109011586 2 4 -0.7078156833918783 2 5 -0.5691387845918143 2 6 -0.35323299049207435 2 7 -0.17882331830176668 2 8 -0.1765276048997393 2 9 -0.30848917044330193 2 10 -0.421897712162874 2 11 -0.7315770643555126 2 12 -0.5918508143064614 2 13 -0.4298619717312633 2 14 -0.40626362199101607 2 15 -0.5154691547042205 2 16 -0.3600295397295028 2 17 -0.6155005473079669 2 18 -0.5894424838341337 2 19 -0.45666790203382135 2 20 -0.4108405119070966 2 21 -0.5118700562213832 2 22 -0.374297033331942 2 23 -0.34308983320324415 2 24 -0.47297501966389177 2 25 -0.7555955532996066 2 26 -0.351639206946075 2 27 -0.3392991005373531 2 28 -0.21813264966286283 2 29 -0.35452409290373055 2 30 -0.07116540735537313 2 31 -0.03468532740875929 2 32 0.3782545852591639 2 33 -0.5290559070250751 2 34 0.24859436571605897 2 35 0.30542131197270805 2 36 0.12111450090841203 2 37 0.3366557933019436 2 38 0.7284393169493484 2 39 0.12495335611605185 2 40 0.2900304753831051 2 41 0.29960742593024037 2 42 -0.03913295606603384 2 43 0.2251324631161289 2 44 0.23200912069128066 2 45 0.5232530518105705 2 46 0.315557418592757 2 47 0.3852147788265725 2 48 0.05569122916966407 2 49 0.483846468375031 2 50 0.30384449249523726 2 51 0.6141115333122578 2 52 0.6446984272727541 2 53 0.7351670228146645 2 54 0.43465791551018995 2 55 0.5502648530154914 2 56 0.5513363786322927 3 1 -0.2583179487540903 3 2 -0.6174640243904861 3 3 -0.3569270313851689 3 4 -0.4548194287102305 3 5 -0.58560378027029 3 6 -0.32516471045749995 3 7 -0.21150122092348767 3 8 -0.1516644530702962 3 9 -0.1772085596683155 3 10 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1.051107529084117 3 51 0.8651631034649512 3 52 0.7808335468348382 3 53 0.9475985987293853 3 54 1.1212107821192348 3 55 1.1532322975559701 3 56 0.12604827722059397 4 1 -0.3224403819195345 4 2 -0.7560192785985862 4 3 -0.5673751780983309 4 4 -0.7666279439282775 4 5 -0.7792625688535324 4 6 -0.4501162796167435 4 7 -0.1316010960795424 4 8 -0.11186506437513088 4 9 -0.22198018134810837 4 10 -0.5944957080367317 4 11 -0.09503860281704846 4 12 0.006469256427401149 4 13 -0.12819677868676393 4 14 -0.10728042128367576 4 15 -0.09354902502547446 4 16 -0.25979376018337774 4 17 -0.1423425750120657 4 18 -0.2672792064984435 4 19 -0.061884524491823356 4 20 -0.14435804801084373 4 21 -0.4091880638842227 4 22 -0.14920861600214863 4 23 -0.11764037844344699 4 24 -0.026165411647390613 4 25 -0.23809629011068978 4 26 0.0930925938202094 4 27 -0.13913700209939606 4 28 -0.2702325612455354 4 29 -0.08949314683830778 4 30 0.0925245425414758 4 31 0.4322171188040258 4 32 0.3002041873811834 4 33 0.1539090207674363 4 34 0.5202418752946016 4 35 0.46094002697417835 4 36 0.20998865357567098 4 37 0.46405042931726825 4 38 0.7938313004447792 4 39 0.24680717279745323 4 40 0.5829128803344759 4 41 0.6666572343593558 4 42 0.584820772198043 4 43 0.48868978701828425 4 44 0.5961264210154766 4 45 0.5869282654857813 4 46 1.0702656414044256 4 47 0.5911018993688657 4 48 0.8634056411944222 4 49 1.0516036154425237 4 50 0.7992025826809162 4 51 0.9162630216706447 4 52 0.44592890177909084 4 53 0.6906783697053207 4 54 0.3639461560053263 4 55 0.7467576322668918 4 56 0.4265132132918872 5 1 -0.15637168216536423 5 2 -0.5281717380143505 5 3 -0.3522399711353401 5 4 -0.12910662751519197 5 5 -0.34015553277619404 5 6 -0.35268089326368113 5 7 -0.1621084820782599 5 8 -0.11752360312117105 5 9 -0.18243685089387895 5 10 0.04543962294903281 5 11 -0.37442203248949824 5 12 -0.4254953970839921 5 13 -0.038213831817230925 5 14 -0.30517424893632494 5 15 -0.44730182107446126 5 16 -0.6965642959174403 5 17 -0.4806146721041742 5 18 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0.08158002034176803 7 28 0.5164971941775036 7 29 0.3937408000980014 7 30 0.8398814405335813 7 31 0.5674546378890994 7 32 0.49770224286471887 7 33 0.48042934456544373 7 34 0.5226545160919969 7 35 0.6589638220941652 7 36 0.7232833846562485 7 37 0.3723700790162283 7 38 0.2581530242946357 7 39 0.5284235701125795 7 40 0.6853456864877927 7 41 0.952990884576856 7 42 1.0233164366465175 7 43 0.8105864827806748 7 44 0.47339142259989586 7 45 0.7759424676578774 7 46 0.956057707777255 7 47 0.8059637666808257 7 48 1.160506151089848 7 49 1.1665821537422936 7 50 1.1392985121597832 7 51 1.133544731692826 7 52 1.4237470102687055 7 53 1.4225007417273297 7 54 1.3924225193274253 7 55 1.7108200872549375 7 56 0.929257142079372 8 1 -0.6790719463768227 8 2 -0.8902106659388522 8 3 -0.5123128888836561 8 4 -0.7364583979627355 8 5 -0.5691311076380288 8 6 -0.4022465019307333 8 7 -0.4446416815752201 8 8 -0.5061913543239669 8 9 -0.6737547930442602 8 10 -0.2793558727647465 8 11 -0.19284299403461347 8 12 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0.16337265044941773 120 41 0.22604420753943089 120 42 -0.14314857646096613 120 43 0.05190817782287738 120 44 0.2436359995595403 120 45 0.2957405260221826 120 46 0.5606870973431235 120 47 0.29225132465622394 120 48 0.32700717376146926 120 49 0.5860863740632105 120 50 0.2532255711866611 120 51 0.9890599565365434 120 52 0.8712162581009295 120 53 1.0470002087857821 120 54 1.1685438571547522 120 55 1.31380744069601 120 56 1.401608998732499 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 287.0 5 26.0 6 43.0 7 150.0 8 305.0 9 426.0 10 415.0 11 611.0 12 747.0 13 925.0 14 1314.0 15 1716.0 16 2207.0 17 2608.0 18 3357.0 19 3991.0 20 4039.0 21 5137.0 22 5311.0 23 5771.0 24 7884.0 25 10257.0 26 9243.0 27 9062.0 28 10582.0 29 11417.0 30 13202.0 31 15664.0 32 19247.0 33 23312.0 34 30634.0 35 41799.0 36 59341.0 37 84177.0 38 102922.0 39 69982.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.56101922377448 0.3461676978235512 0.061098957017510856 0.03171412138445578 8 99.35778632549102 0.5296515233530615 0.05717727104894303 0.05538488010696989 9 96.03286801371054 2.256361189799162 0.7564803417241374 0.9542904547661666 10 45.7699274875428 40.62417691104458 6.211846747331624 7.394048854080998 11 39.251331724334406 23.17764745722625 20.10800718853418 17.463013629905163 12 35.85147040642453 15.644916513023396 23.28389872265553 25.21971435789655 13 28.51690792691776 16.644359276201328 27.06594207962215 27.772790717258754 14 24.83072363741263 20.874521376572044 29.474244370743452 24.820510615271875 15 30.566141860669294 20.078801528728157 26.789832130167657 22.565224480434896 16 34.308229008208045 21.457559517730342 23.034127619774562 21.20008385428705 17 32.734706895223354 19.988855263558683 24.82427330763952 22.452164533578443 18 31.337852147691052 21.169623963691812 23.48690493468145 24.005618953935688 19 31.81696830917147 23.42329751608551 21.95925183341665 22.800482341326365 20 30.479872999036022 25.913536138296312 22.474225326371695 21.13236553629597 21 29.619018438984362 22.253637717228294 26.944460868895252 21.18288297489209 22 28.81666908554033 20.309579993943856 27.242967796728607 23.630783123787204 23 29.036211499525184 22.08919388650983 25.66160792675279 23.212986687212197 24 30.075600241548056 21.36105521380049 24.014320861115245 24.549023683536205 25 29.057146851241832 21.362591985891612 23.228655509475487 26.35160565339107 26 25.447256218914394 20.48449619352595 25.38342650797692 28.68482107958274 27 24.335038431954732 19.355110544537858 25.835561077851825 30.474289945655585 28 25.904608508180665 23.879799258879277 24.5954506810347 25.620141551905355 29 29.803730132521643 21.649628853249865 24.33146922425304 24.215171789975447 30 29.04618125843076 19.894349437170963 27.942616045881298 23.116853258516976 31 30.91947433964502 20.967292683847248 26.425160966861398 21.68807200964633 32 33.207301009396716 20.008230437884453 26.019864467330734 20.764604085388097 33 31.060347337807766 19.845563731922223 27.252465192524078 21.841623737745934 34 26.565354024939236 23.741712091357563 30.59191128881474 19.101022594888466 35 25.08741195853148 27.44231889896904 29.121851550752893 18.348417591746585 36 26.968038879462508 27.664911141139577 24.921505157718403 20.44554482167951 37 27.052009184018655 26.538647012674808 25.86028199831578 20.54906180499075 38 29.01726403715638 25.566256818304563 24.590181641433688 20.82629750310537 39 28.51534007510198 22.041441165611577 24.79986312348603 24.64335563580041 40 26.30780211382348 23.173835306331377 26.12738453996222 24.390978039882917 41 23.618181949291873 23.632243502841156 26.79086503793014 25.958709509936835 42 23.63236736577907 22.073248132013962 27.20786573579898 27.086518766407984 43 23.374927399775615 20.60800207789959 28.952608738000674 27.064461784324124 44 25.102814594621897 21.000692645690084 26.931832120000866 26.964660639687153 45 25.25360422944371 21.988957254470325 27.12960791035889 25.62783060572707 46 23.85802012296162 23.587439575157852 26.48765149081037 26.066888811070154 47 23.23287958190821 23.634501962965505 28.768550656067493 24.364067799058788 48 25.428763470002817 22.877864747078338 28.91524468246569 22.778127100453155 49 27.03998323796312 21.76554100919022 27.034564476041844 24.15991127680481 50 24.67122148718505 21.873802552145467 27.930267866825726 25.524708093843763 51 22.92258019054571 23.580799439167567 28.80824915125311 24.688371219033613 52 23.48226065896727 21.898642664787 29.90041388783459 24.71868278841114 53 22.9914579349386 21.039941293636648 31.898811051545202 24.06978971987955 54 23.35004580297255 21.906022863241247 28.87608793442253 25.86784339936368 55 23.93526990763177 23.24974141235868 26.339592480343722 26.475396199665823 56 24.36530694157678 23.42334686911275 25.356624998644012 26.85472119066646 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 11.0 5 22.0 6 45.5 7 69.0 8 78.0 9 87.0 10 645.5 11 1204.0 12 1046.0 13 820.5 14 753.0 15 742.0 16 731.0 17 1284.0 18 1837.0 19 1981.0 20 2125.0 21 2312.0 22 2996.5 23 3494.0 24 4072.5 25 4651.0 26 5424.0 27 6197.0 28 6862.0 29 8219.5 30 8912.0 31 9524.5 32 10137.0 33 9539.5 34 8942.0 35 9426.0 36 9910.0 37 9015.5 38 8883.0 39 9645.0 40 10174.5 41 10704.0 42 11312.0 43 11920.0 44 13207.0 45 14494.0 46 17497.5 47 19879.5 48 19258.0 49 20325.5 50 21393.0 51 22662.5 52 23932.0 53 25168.0 54 27893.5 55 29383.0 56 31487.5 57 33592.0 58 36905.5 59 40219.0 60 45874.5 61 51530.0 62 52255.5 63 42101.5 64 31222.0 65 27732.0 66 24242.0 67 21013.5 68 17785.0 69 15670.0 70 13555.0 71 11516.0 72 8139.5 73 6802.0 74 5659.5 75 4517.0 76 3656.5 77 2796.0 78 2184.0 79 1293.5 80 1015.0 81 775.0 82 535.0 83 409.0 84 283.0 85 215.5 86 148.0 87 103.5 88 43.0 89 27.0 90 17.0 91 7.0 92 6.5 93 6.0 94 3.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.035297637925072255 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 8.958791351541181E-4 21 0.0 22 0.0 23 1.791758270308236E-4 24 0.007883736389356239 25 0.025980494919469424 26 0.0675492867906205 27 0.06665340765546639 28 0.10338445219678523 29 0.1646625850413269 30 0.24547088303222836 31 0.29205659806024253 32 0.29384835633055073 33 0.31624533470940364 34 0.3366713789909176 35 0.38039028078643855 36 0.4196297869061889 37 0.4233924792738362 38 0.4696198426477887 39 0.5135179202703405 40 0.5889509434503173 41 0.6104520426940161 42 0.6276529220889752 43 0.6638464391492015 44 0.6656381974195097 45 0.6989649012472429 46 0.7376668798859009 47 0.7806690783732986 48 0.8347801781366073 49 0.802528529271059 50 0.8691819369265253 51 0.8328092440392681 52 0.8638066621156006 53 0.8688235852724636 54 1.6161659598180291 55 0.9150509486464162 56 0.8974917175973954 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 558111.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.3140754680126 #Duplication Level Percentage of deduplicated Percentage of total 1 66.73922891435078 23.568341665583517 2 12.735827005130346 8.995079120134518 3 6.1711708623206585 6.537875806739763 4 3.663436087905065 5.174834339220811 5 2.4364870270710144 4.3021143375409725 6 1.6418339468219572 3.4787908742409344 7 1.1799625171503771 2.9168499766052167 8 0.8735270640805815 2.46782405314345 9 0.6464539091354697 2.054602992042156 >10 3.572041744111101 22.979961668772134 >50 0.21610398953541402 5.199917373643131 >100 0.11317294238665448 7.141432047181034 >500 0.007169326667027887 1.6914108365987395 >1k 0.0035846633335139435 3.490964908553626 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 4880 0.8743780359104192 No Hit GAGATCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 4693 0.8408721562556553 No Hit GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 3305 0.5921761083368721 No Hit GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 2234 0.4002787975868599 No Hit GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1420 0.25442967438376957 No Hit GAGATCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG 1414 0.2533546194215846 No Hit GAGATCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 1358 0.24332077310785846 No Hit GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 932 0.16699187079272762 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 906 0.1623332992899262 No Hit GAGATCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA 835 0.14961181557073772 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 762 0.13653198019748758 No Hit GAGATCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA 689 0.12345214482423748 No Hit GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 653 0.11700181505112782 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC 637 0.11413500181863465 No Hit GAGATCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 630 0.11288077102941887 No Hit GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 578 0.10356362802381605 No Hit GAGATCGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCAT 570 0.10213022140756947 No Hit GAGATCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 569 0.10195104558053865 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.13993632091107325 0.0 0.0 0.0 0.0 3 0.13993632091107325 0.0 0.0 0.0 0.0 4 0.13993632091107325 0.0 0.0 0.0 0.0 5 0.13993632091107325 0.0 0.0 0.0 0.0 6 0.13993632091107325 0.0 0.0 0.0 0.0 7 0.14065302421919654 0.0 0.0 0.0 0.0 8 0.14065302421919654 0.0 0.0 0.0 0.0 9 0.14065302421919654 0.0 0.0 0.0 0.0 10 0.14441571658684382 0.0 0.0 0.0 0.0 11 0.1479992331274603 0.0 0.0 0.0 0.0 12 0.1494326397437069 0.0 0.0 0.0 0.0 13 0.1517619254951076 0.0 0.0 0.0 0.0 14 0.1562413211708782 0.0 0.0 0.0 0.0 15 0.1578539036141556 0.0 0.0 0.0 0.0 16 0.15982483771149467 0.0 0.0 0.0 0.0 17 0.16269165094398785 0.0 0.0 0.0 0.0 18 0.16663351913866598 0.0 0.0 0.0 0.0 19 0.16985868402522078 0.0 0.0 0.0 0.0 20 0.1752339588361455 0.0 0.0 0.0 0.0 21 0.17917582703082363 0.0 0.0 0.0 0.0 22 0.18401357436065585 0.0 0.0 0.0 0.0 23 0.1890304975175189 0.0 0.0 0.0 0.0 24 0.19333071736625868 0.0 0.0 0.0 0.0 25 0.19673505807984434 0.0 0.0 0.0 0.0 26 0.19996022296639915 0.0 0.0 0.0 0.0 27 0.20228950871779985 0.0 0.0 0.0 0.0 28 0.21196500337746435 0.0 0.0 0.0 0.0 29 0.2146526407829267 0.0 0.0 0.0 0.0 30 0.22092379472900553 0.0 0.0 0.0 0.0 31 0.224507311269622 0.0 0.0 0.0 0.0 32 0.22719494867508436 0.0 0.0 0.0 0.0 33 0.22916588277242342 0.0 0.0 0.0 0.0 34 0.2313159926967933 0.0 0.0 0.0 0.0 35 0.2388413774320879 0.0 0.0 0.0 0.0 36 0.24188736649161188 0.0 0.0 0.0 0.0 37 0.242783245626766 0.0 0.0 0.0 0.0 38 0.24672511382144413 0.0 0.0 0.0 0.0 39 0.24851687209175236 0.0 0.0 0.0 0.0 40 0.25210038863236883 0.0 0.0 0.0 0.0 41 0.25604225682704695 0.0 0.0 0.0 0.0 42 0.2592674217136018 0.0 0.0 0.0 3.5835165406164726E-4 43 0.2626717624271874 0.0 0.0 0.0 3.5835165406164726E-4 44 0.2662552789678039 0.0 0.0 0.0 3.5835165406164726E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGTTG 15 0.007963731 50.722946 48 CGACGTT 15 0.008427695 50.00153 30 CAATTCG 15 0.008454716 49.961052 26 CGGTAAG 15 0.008575584 49.781948 6 TATTTCG 15 0.00858774 49.76411 15 GATAACG 15 0.00858774 49.76411 9 ATAACGA 15 0.00858774 49.76411 10 ATTTCGG 15 0.00858774 49.76411 16 TCGGGAT 6180 0.0 48.734753 5 CGGGATA 640 0.0 48.615185 6 ATCGGAT 315 0.0 48.201572 4 TCGGGAC 3780 0.0 48.20157 5 ATCGGGA 23015 0.0 47.759525 4 CGGGATT 1720 0.0 47.75594 6 TCGGGAG 8375 0.0 47.55291 5 GATCGGG 55675 0.0 47.452682 3 AGATCGG 58030 0.0 47.392796 2 CGGGATC 1865 0.0 47.379604 6 GAGATCG 58420 0.0 47.366196 1 ATCGGGG 25540 0.0 47.189545 4 >>END_MODULE