FastQCFastQC Report
Fri 17 Jun 2016
SRR1526678_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526678_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115521
Sequences flagged as poor quality0
Sequence length64
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6940.6007565723980921No Hit
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.4328217380389712No Hit
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.28739363405787693No Hit
AAGTACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2660.23026116463673274No Hit
AAGTACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.12898087793561344No Hit
AAGTACGGGGGAATTTCTTTTCTGGTCCAGTCTATTTGGAGTTCTGTAGGCTTCTTGTATGATC1490.12898087793561344No Hit
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.12119008665091195No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1230.10647414755758694No Hit
AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.10041464322504134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGAT201.9125841E-458.54254557
TACGGAA453.092282E-1158.1092764
CGGGTAC2050.058.1092726
TACGGTA150.004641858.1092724
TACGGCA201.9841813E-458.1092724
TACGGAT201.9841813E-458.1092724
TACGGAC201.9841813E-458.1092724
TACGAGA351.599983E-858.1092724
ACGGATG150.004641858.1092725
TACAGGG406.9849193E-1058.1092724
ACGAGAC150.004641858.1092725
ACGGTGT150.004641858.1092725
TCATTTG150.004649810558.0839947
AATATTC150.004649810558.0839947
CGCTCTT150.004649810558.0839949
TACTCTT150.004649810558.0839948
TTGTATG258.54664E-658.08398455
TGAGTTA258.54664E-658.08398441
TTGAGTT258.54664E-658.08398440
AACTTTC201.9970343E-458.03347839
Produced by FastQC (version 0.11.5)