FastQCFastQC Report
Fri 17 Jun 2016
SRR1526677_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526677_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163644
Sequences flagged as poor quality0
Sequence length64
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT19361.183055901835692No Hit
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7310.4467013761580015No Hit
AAGTACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4290.2621544327931363No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3950.24137762459974088No Hit
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.22548947715773263No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3590.21937865121849867No Hit
AAGTACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3460.21143457749749456No Hit
AAGTACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3360.2053237515582606No Hit
AAGTACGGGGATGTATGAAGACAGCATGCTGGTCAATCTTTAGCATAAAATAGACCGAGTTATT2840.1735474566742441No Hit
AAGTACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2820.1723252914863973No Hit
AAGTACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2670.16315905257754637No Hit
AAGTACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2330.14238224438415098No Hit
AAGTACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC2210.1350492532570702No Hit
AAGTACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2090.1277162621299895No Hit
AAGTACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1990.12160543619075553No Hit
AAGTACGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC1910.11671677543936838No Hit
AAGTACGGGGAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1870.11427244506367482No Hit
AAGTACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1870.11427244506367482No Hit
AAGTACGGGCATCCAGACTTTGGTTTACATACAGGAGAAACTTTCCAGCTATTGGGGGAACTGG1860.1136613624697514No Hit
AAGTACGGGGGAATTTCTTTTCTGGTCCAGTCTATTTGGAGTTCTGTAGGCTTCTTGTATGATC1830.11182811468798122No Hit
AAGTACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1760.10755053653051747No Hit
AAGTACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1750.10693945393659408No Hit
AAGTACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1750.10693945393659408No Hit
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1740.10632837134267067No Hit
AAGTACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1650.10082862799736013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTA201.9654437E-458.2355354
CGTAGTA201.9654437E-458.2355357
GTTCAAG201.9654437E-458.2355354
AACCGTT150.00460551658.23552757
GGTACTC150.00460551658.23552756
CGAGTTA351.5783371E-858.23552756
TATGATC258.431305E-658.23552758
AACGTAG201.9684383E-458.2176155
GCCCCAA150.004611134558.21760653
TCTTGTA258.446712E-658.21760653
ATATCTT150.004611134558.21760653
ATTCAAG150.004611134558.21760655
GCTATTG258.477598E-658.1817948
TTAAATG150.004622387258.1817947
CTATCGG150.00462802158.16389549
TCGACCC150.00462802158.16389549
CCCCCTA150.0046336658.1460245
CCTATAT201.9804535E-458.1460245
GCATGTT150.0046336658.1460245
CGCAACT150.004639304258.12814739
Produced by FastQC (version 0.11.5)