FastQCFastQC Report
Fri 17 Jun 2016
SRR1526676_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526676_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162653
Sequences flagged as poor quality0
Sequence length64
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT17671.086361764000664No Hit
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.43712688975918057No Hit
AAGTACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3950.24284827208843365No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3780.23239657430234917No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3670.2256337110290004No Hit
AAGTACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3360.20657473271319926No Hit
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.18936017165376598No Hit
AAGTACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2920.17952327961980413No Hit
AAGTACGGGGATGTATGAAGACAGCATGCTGGTCAATCTTTAGCATAAAATAGACCGAGTTATT2650.16292352431249346No Hit
AAGTACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2340.14386454599669235No Hit
AAGTACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC2090.12849440219362693No Hit
AAGTACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2080.12787959644150432No Hit
AAGTACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2080.12787959644150432No Hit
AAGTACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1920.11804270440754244No Hit
AAGTACGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC1900.1168130929032972No Hit
AAGTACGGGCATCCAGACTTTGGTTTACATACAGGAGAAACTTTCCAGCTATTGGGGGAACTGG1810.11127984113419366No Hit
AAGTACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1730.10636139511721271No Hit
AAGTACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1710.10513178361296747No Hit
AAGTACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1710.10513178361296747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATTG258.151235E-658.5677745
CGTACTG258.1814815E-658.53121642
CCGATTG150.004513980458.5312143
TACGTAC258.196641E-658.5129540
CAACGCG150.004603926658.24037622
GGCTTAT150.004603926658.24037624
AACGAGC258.4268195E-658.24037623
ACACGGT150.00461526358.20422420
CCCTATT150.00462093958.18616558
CACGCGA150.00462093958.18616558
AGTGCGT150.00462093958.18616558
TCGACGC303.65937E-758.18616558
AGTTATT258.473493E-658.1861658
CGAGTTA258.489098E-658.16811856
TAACGTA150.004632306858.15007854
TCTCACG150.004632306858.15007855
TCGCTAC258.520381E-658.13205352
CTCGCTA258.520381E-658.13205351
TTAACGT150.00463799858.13205353
GAGACAT201.9857992E-458.1140447
Produced by FastQC (version 0.11.5)