FastQCFastQC Report
Fri 17 Jun 2016
SRR1526675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146671
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG20311.3847318147418373No Hit
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT7430.5065759420744387No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6590.4493049069004779No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5260.3586257678750401No Hit
AAGTACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA4480.30544552092779076No Hit
AAGTACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG3170.2161299779779234No Hit
AAGTACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3030.20658480544892993No Hit
AAGTACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2920.1990850270332922No Hit
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.1990850270332922No Hit
AAGTACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT2620.17863108589973478No Hit
AAGTACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2510.17113130748409705No Hit
AAGTACGGGGATGTATGAAGACAGCATGCTGGTCAATCTTTAGCATAAAATAGACC2220.1513591643883249No Hit
AAGTACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA2050.1397685977459757No Hit
AAGTACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT2010.1370414055948347No Hit
AAGTACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC2010.1370414055948347No Hit
AAGTACGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT2000.13635960755704946No Hit
AAGTACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1890.12885982914141175No Hit
AAGTACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1880.1281780311036265No Hit
AAGTACGGGCTCCTCCTCCTCGCCCGGCCCCAGCTGCCAGCACCATGTCCGCAGGG1870.12749623306584124No Hit
AAGTACGGGGACACAACTGCAGTTGTGAGCATGCTCTGTGTGGTGTGACAGAGTTC1740.11863285857463303No Hit
AAGTACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1690.11522386838570678No Hit
AAGTACGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG1620.11045128212121005No Hit
AAGTACGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1610.10976948408342481No Hit
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.10908768604563955No Hit
AAGTACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.10840588800785432No Hit
AAGTACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG1580.10772408997006906No Hit
AAGTACGGGGGAATTTCTTTTCTGGTCCAGTCTATTTGGAGTTCTGTAGGCTTCTT1550.10567869585671333No Hit
AAGTACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.10431509978114284No Hit
AAGTACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1530.10431509978114284No Hit
AAGTACGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA1470.10022431155443133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACC402.3701432E-950.64610350
TGAGGGT203.91557E-450.64610349
AATAGAC451.1823431E-1050.64610349
GCGTATC203.91557E-450.64610348
TCGCACT150.00799971750.646149
CGTATCA150.00799971750.646149
CGGCATG150.00799971750.646149
CTCATGG150.00799971750.646150
AGCGTAT150.0082436950.26281447
CCGATTG252.0231622E-550.2282643
CCCGATT252.0231622E-550.2282642
CTCACAG150.00826614250.22825642
ATTGTTC150.00826614250.22825646
AGCATAA550.050.22825642
ATTGCTC301.0093136E-650.22825646
ACCCGAT252.0314106E-550.19375241
AACCGAC204.0929203E-450.1937541
ATACCTA150.00828863850.1937541
CAACCGA204.0998665E-450.1765140
AGTGAAT150.00829990250.1765140
Produced by FastQC (version 0.11.5)