FastQCFastQC Report
Fri 17 Jun 2016
SRR1526674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72957
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT54507.470153652151266No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT3710.5085187165042422No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT2420.3317022355634141No Hit
ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2270.31114217964006197No Hit
ATCAACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2120.29058212371670983No Hit
ATCAACGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT1570.2151952519977521No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT1510.20697122962841127No Hit
ATCAACGGGCAGATTCAGAACGTGGGAGGTTTTGTGTCTGGCTTATTTCCTTTTGCATATTATA1130.1548857546225859No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAC1120.15351508422769577No Hit
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.1343256986992338No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTACGCCATGCAT870.11924832435544225No Hit
ATCAACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA870.11924832435544225No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAG860.11787765396055211No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAGCCATGCAT860.11787765396055211No Hit
ATCAACGGGATTGCATATCTTGATATATTTTTCTGTTTGTTTGTTTGTTTGTTTTTTGGCTTTT770.10554162040654085No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGT150.00449886658.5478558
CACGCAT303.4786353E-758.5478558
GTAGACA150.00449886658.5478558
CTAATTC150.00449886658.5478558
AATAATT201.9135451E-458.5072857
GTCATGC150.004511243758.50727556
CCATGCG150.004511243758.50727557
CGCTAAT150.004511243758.50727556
GCTAATT150.004511243758.50727557
GGTAGAC150.004511243758.50727557
CGGGTCA150.00459859858.2248276
CGGGTAT201.9599355E-458.2248276
CAACGGC150.00459859858.2248273
CGGGGTA650.058.2248276
CGGGCTA150.00459859858.2248276
CGGGCAT750.058.2248276
CGGGATT1150.058.2248276
CGGGACT1200.058.2248276
AACAGGA150.00459859858.2248274
GGGATGG550.058.2248277
Produced by FastQC (version 0.11.5)