FastQCFastQC Report
Fri 17 Jun 2016
SRR1526673_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526673_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107365
Sequences flagged as poor quality0
Sequence length64
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1590014.809295394215994No Hit
ATCAACGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT6460.6016858380291529No Hit
ATCAACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG5070.4722209286080194No Hit
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT4180.38932613048945186No Hit
ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3940.3669724770642202No Hit
ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3310.308294136822987No Hit
ATCAACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.2784892655893447No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2840.26451823219857495No Hit
ATCAACGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG2510.23378195873888138No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2280.21235970753970104No Hit
ATCAACGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG2130.19838867414893122No Hit
ATCAACGGGCAGATTCAGAACGTGGGAGGTTTTGTGTCTGGCTTATTTCCTTTTGCATATTATA1940.18069203185395613No Hit
ATCAACGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG1910.17789782517580216No Hit
ATCAACAGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1710.15926978065477576No Hit
ATCAACGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT1690.15740697620267313No Hit
ATCAACGGGTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA1660.15461276952451916No Hit
ATCAACGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC1620.15088716062031388No Hit
ATCAACGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG1440.1341219205513901No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1430.1331905183253388No Hit
ATCAACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG1390.1294649094211335No Hit
ATCAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA1240.11549387603036372No Hit
ATCAACGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCG1210.11269966935220975No Hit
ATCAACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1190.11083686490010712No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATTGCTTGTCTCAAAGATTAAGCCATGCATG1140.10617985376985052No Hit
ATCAACGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT1140.10617985376985052No Hit
ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1100.10245424486564524No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC201.9605257E-458.24660558
CATGCGA150.004597726358.246657
ATGCGAG150.004597726358.246658
ATGAGGA201.9650774E-458.21930753
TTGCATA258.42547E-658.21930753
TTCGTAA150.004606271658.21930350
TCGTAAG150.004606271658.21930351
CGTAAGG150.004606271658.21930352
GTAAGGC150.004606271658.21930353
GATACAG201.9696375E-458.1920447
TTATCTC201.9696375E-458.1920447
TATCTCA201.9696375E-458.1920448
ATACAGA201.9696375E-458.1920448
CGATACA201.974206E-458.164846
TATTTCC201.9833683E-458.11038244
TTTAAGC150.00464057258.11038244
TAGCACG201.9833683E-458.11038241
ACGATAC201.9833683E-458.11038245
TTAAGCA150.00464057258.11038245
AACGATA201.9833683E-458.11038244
Produced by FastQC (version 0.11.5)