FastQCFastQC Report
Fri 17 Jun 2016
SRR1526672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106241
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1491514.03883623083367No Hit
ATCAACGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT6230.5864026129272126No Hit
ATCAACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG5040.47439312506471143No Hit
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT3980.37461996780903795No Hit
ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3540.33320469498592825No Hit
ATCAACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3230.30402575276964633No Hit
ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3070.2889656535612429No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2610.24566786833708268No Hit
ATCAACGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG2130.20048757071187204No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1970.18542747150346853No Hit
ATCAACGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG1920.18072119050084243No Hit
ATCAACGGGTACCTGGTTGATCCTGNCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1700.16001355408928755No Hit
ATCAACGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG1660.15624852928718666No Hit
ATCAACGGGCAGATTCAGAACGTGGGAGGTTTTGTGTCTGGCTTATTTCCTTTTGCATATTATA1650.15530727308666145No Hit
ATCAACGGGTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGA1610.15154224828456056No Hit
ATCAACAGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1530.1440121986803588No Hit
ATCAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA1500.14118843007878315No Hit
ATCAACGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC1410.13271712427405616No Hit
ATCAACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1360.12801084327143003No Hit
ATCAACGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT1310.12330456226880394No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1240.11671576886512741No Hit
ATCAACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGG1210.11389200026355173No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCNCAAAGATTAAGCCATGCAT1170.11012697546145085No Hit
ATCAACGGGTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT1150.1082444630604004No Hit
ATCAACGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCG1110.10447943825829951No Hit
ATCAACGGGATGTCAGATCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.10447943825829951No Hit
ATCAACGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG1100.1035381820577743No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATTGCTTGTCTCAAAGATTAAGCCATGCATG1090.1025969258572491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC150.004485974658.6088243
CTGCGTA150.004485974658.6088243
TTATACT150.004485974658.6088243
ACGATCG150.004485974658.6088244
ATTATAC150.004485974658.6088242
TATACTA150.004485974658.6088244
CGATCGT150.004485974658.6088245
TGCGTAG150.004485974658.6088244
GCTCAAA150.004485974658.6088243
TAAATAG258.097699E-658.60881442
TACATGA150.004589089758.27359824
TGTCTTA150.004589089758.27359824
TGGGACG303.6093115E-758.27359825
TTGGGAC303.6093115E-758.27359824
CTGACAG201.955893E-458.2735923
TTTCCGC150.004597761758.24583422
ATCCTGA150.00460644658.21809820
TAGGTCC201.9651353E-458.21809420
TTAGGAT150.00461514258.19038854
AAATCAA150.00461514258.19038857
Produced by FastQC (version 0.11.5)