FastQCFastQC Report
Fri 17 Jun 2016
SRR1526671_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526671_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98468
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1692317.186294024454646No Hit
ATCAACGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC5440.5524637445667628No Hit
ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4490.4559857009383759No Hit
ATCAACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC4280.4346589755047325No Hit
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3580.3635698907259211No Hit
ATCAACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3180.3229475565666003No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA3070.3117764146727871Illumina Single End Adapter 1 (95% over 21bp)
ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2780.28232522240727953No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2400.2437340049559248No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC2090.21225169598245117No Hit
ATCAACGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT1950.19803387902668887No Hit
ATCAACGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC1710.1736604785310964No Hit
ATCAACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1650.16756712840719828No Hit
ATCAACGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT1540.15639598651338507No Hit
ATCAACAGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1510.153349311451436No Hit
ATCAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA1470.14928707803550392No Hit
ATCAACGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG1440.14624040297355487No Hit
ATCAACGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1390.14116261120363977No Hit
ATCAACGGGTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG1380.14014705284965673No Hit
ATCAACGGGCAGATTCAGAACGTGGGAGGTTTTGTGTCTGGCTTATTTCCTTTTGC1330.13506926107974165No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTNAG1300.13202258601779257No Hit
ATCAACGGGGGCCATCTAACCCATCCCTGGCCTGGCTGCGGGGCGCTTCCCGCAAC1260.1279603526018605No Hit
ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATTGCTTGTCTCAAAGATTAAGC1200.1218670024779624No Hit
ATCAACGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC1100.11171141893813218No Hit
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1100.11171141893813218No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1100.11171141893813218No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1100.11171141893813218No Hit
ATCAACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1070.10866474387618312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCT150.00802993150.58526650
CACTCTA203.9324348E-450.58526249
ATCTCGG150.0083117450.1460747
TAGAATG150.0083117450.1460747
GACGTCG150.0083117450.1460746
ACGCTCG150.0083117450.1460745
ACGTCGC150.0083117450.1460747
GATCTCG150.0083117450.1460746
CGTTTCA150.0083117450.1460745
ATCCGTT150.00832854350.12047242
GAAACGC150.00832854350.12047242
GTGACGT150.00832854350.12047244
CTATACA204.1158707E-450.1204742
TATACAA204.1158707E-450.1204743
ATACAAG204.1158707E-450.1204744
AATCCGT150.00834537250.094941
GCTATAC204.1262573E-450.094941
TCAATCC150.00834537250.094939
TGCTATA204.1262573E-450.094940
CTGGGCT150.00834537250.094939
Produced by FastQC (version 0.11.5)