##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526671_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 98468 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.57356704716253 39.0 38.0 40.0 36.0 40.0 2 38.57867530568306 39.0 39.0 40.0 36.0 40.0 3 38.42989600682455 39.0 39.0 40.0 36.0 40.0 4 38.42495023764066 39.0 38.0 40.0 36.0 40.0 5 38.53212211073648 39.0 39.0 40.0 36.0 40.0 6 38.34508672868343 39.0 38.0 40.0 35.0 40.0 7 38.68752285006296 39.0 39.0 40.0 38.0 40.0 8 38.65681236543852 39.0 39.0 40.0 38.0 40.0 9 38.648677743023114 39.0 39.0 40.0 37.0 40.0 10 37.86537758459601 39.0 38.0 40.0 35.0 40.0 11 37.73258317422919 39.0 38.0 40.0 34.0 40.0 12 37.514268594873464 39.0 37.0 40.0 33.0 40.0 13 37.462901653329 39.0 38.0 40.0 33.0 40.0 14 37.57165779745704 39.0 38.0 40.0 33.0 40.0 15 37.44077263679571 39.0 37.0 40.0 33.0 40.0 16 37.39382337409108 39.0 37.0 40.0 33.0 40.0 17 37.10355648535565 39.0 36.0 40.0 33.0 40.0 18 37.26294836901328 39.0 37.0 40.0 33.0 40.0 19 36.85954827964415 38.0 36.0 40.0 32.0 40.0 20 36.807145468578625 38.0 36.0 40.0 32.0 40.0 21 36.77438355607913 38.0 36.0 40.0 32.0 40.0 22 36.773002396717715 38.0 36.0 40.0 32.0 40.0 23 36.77838485599383 38.0 36.0 40.0 32.0 40.0 24 36.322835845147665 38.0 35.0 39.0 31.0 40.0 25 36.03956615347118 38.0 35.0 39.0 30.0 40.0 26 35.799193646666936 38.0 35.0 39.0 30.0 40.0 27 35.745968233334686 38.0 35.0 39.0 30.0 40.0 28 34.86101068367388 38.0 33.0 39.0 27.0 40.0 29 35.136816021448595 38.0 35.0 39.0 29.0 40.0 30 34.40213064142666 37.0 33.0 39.0 27.0 40.0 31 34.12181622456026 37.0 33.0 39.0 26.0 40.0 32 33.946073851403504 37.0 33.0 39.0 25.0 40.0 33 33.601484746313524 37.0 33.0 39.0 23.0 40.0 34 33.77336799772515 37.0 33.0 39.0 24.0 40.0 35 33.46126660437909 37.0 33.0 39.0 23.0 40.0 36 33.38467319332169 37.0 33.0 39.0 22.0 40.0 37 32.95961124426209 36.0 32.0 39.0 19.0 40.0 38 33.135272372750535 37.0 33.0 39.0 19.0 40.0 39 32.95337571596864 37.0 32.0 39.0 17.0 40.0 40 32.406426453264004 36.0 32.0 39.0 13.0 40.0 41 32.15187675183816 36.0 31.0 39.0 4.0 40.0 42 31.718629402445465 36.0 31.0 39.0 4.0 40.0 43 31.279105902425155 36.0 30.0 39.0 4.0 40.0 44 31.451628955599787 36.0 31.0 39.0 4.0 40.0 45 30.853678352358127 35.0 30.0 39.0 4.0 40.0 46 30.57186090912784 36.0 30.0 39.0 4.0 40.0 47 29.936476824958362 35.0 29.0 38.0 4.0 39.0 48 29.94626680749076 35.0 29.0 39.0 4.0 40.0 49 29.593106389893162 35.0 28.0 38.0 4.0 39.0 50 29.22788113905025 35.0 27.0 38.0 4.0 40.0 51 28.079792419872447 34.0 24.0 38.0 4.0 39.0 52 27.959550310760857 34.0 23.0 38.0 4.0 39.0 53 27.862300442783443 34.0 23.0 38.0 4.0 39.0 54 27.288570906284274 34.0 18.0 38.0 4.0 39.0 55 27.02747085347524 34.0 4.0 38.0 4.0 39.0 56 15.938660275419426 20.0 4.0 23.0 4.0 25.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.790170559600412 1 2 -1.3074407217608695 1 3 -0.6627803006390067 1 4 -0.8868667541388504 1 5 -1.4791301125393943 1 6 -0.36780966108130997 1 7 -0.40296089072442953 1 8 -0.46850860055027255 1 9 -0.7855842435607983 1 10 -0.3243546042822132 1 11 -0.4903076010205183 1 12 -0.5441113585593413 1 13 -0.4170414227096799 1 14 -0.82283039157263 1 15 -0.9991484884361697 1 16 0.07776786434775573 1 17 0.008496954288133907 1 18 -0.17617637354206295 1 19 0.16130378886310126 1 20 0.20493137366062797 1 21 0.24364181037591948 1 22 0.022760861443437364 1 23 0.14501990740623683 1 24 0.07932156194630835 1 25 -0.3289118222831391 1 26 0.6610225919695552 1 27 -0.13022152376301221 1 28 0.06716887117850234 1 29 -0.15852867413358496 1 30 1.2358508406437991 1 31 0.04373413055295572 1 32 0.057932415925428415 1 33 0.6506231260579796 1 34 -0.610996145567043 1 35 -0.5949046784908916 1 36 -0.03040197846402748 1 37 0.768724235838306 1 38 -0.5874439547739456 1 39 -0.0487061328076237 1 40 0.3108237088179422 1 41 1.3045974257560857 1 42 0.7445188971830738 1 43 0.835158624108093 1 44 1.3325230678613558 1 45 9.583656121812112E-4 1 46 0.5509137175694505 1 47 1.5006125109807336 1 48 1.086519568107331 1 49 0.8042420635738807 1 50 1.681262995947229 1 51 1.9941840002534335 1 52 3.0151175840542166 1 53 2.481108355569038 1 54 2.345277617791883 1 55 1.288316284664642 1 56 2.6065446803798142 2 1 -0.16944900548385533 2 2 -0.0898181500679911 2 3 -0.10450092968618208 2 4 -0.08991948309351727 2 5 -0.19328367405650937 2 6 -0.1867273299064749 2 7 0.0059197753255233465 2 8 -0.14265291137358105 2 9 -0.03835945170141031 2 10 0.24872587675385915 2 11 -0.8147756861269002 2 12 -0.8514656601319643 2 13 -0.17305437368099774 2 14 -0.5612670243200881 2 15 -0.31737235522618334 2 16 0.013475542423613263 2 17 0.1858790079421624 2 18 -0.31655565198794733 2 19 -0.27625402797316667 2 20 -0.2317013737954312 2 21 -1.319494174822971 2 22 -1.7596165668636843 2 23 -1.3845823127602728 2 24 -0.8434351448066977 2 25 -1.6205399443922985 2 26 -0.9782003497510701 2 27 -0.8372057975835503 2 28 -0.4006849678594264 2 29 -0.5419699322279428 2 30 0.02863529947821064 2 31 -0.5890133255062509 2 32 -0.03850606696078529 2 33 -0.09058871483009057 2 34 -0.3170553500073723 2 35 0.1729029070780257 2 36 0.6957774849957303 2 37 1.199806567013141 2 38 1.0501601155313267 2 39 1.3146148662673127 2 40 0.48751196043681233 2 41 1.3901663434249087 2 42 0.7128352709111034 2 43 1.2581465982061495 2 44 0.3577312084718969 2 45 0.8857872277768415 2 46 0.6443457249700053 2 47 0.16203711782624453 2 48 0.44799042842185344 2 49 0.7656204169503837 2 50 -0.03844098185110667 2 51 1.5365059243977441 2 52 1.0895856691605985 2 53 0.9198225091305545 2 54 1.267571789854788 2 55 1.1745327508996084 2 56 1.0422523584556718 3 1 -0.15792418278881826 3 2 0.19165348113768488 3 3 0.2534334674769383 3 4 -0.26367834834174886 3 5 -0.07559750384374553 3 6 0.059726570802745016 3 7 0.2206804136233984 3 8 0.2529587907540787 3 9 0.3339809738305064 3 10 -0.004155328919900114 3 11 -0.3028076010205183 3 12 -1.801991390522062E-4 3 13 0.519099881638148 3 14 -0.050185464036403005 3 15 0.2943297724333931 3 16 0.28519540057963866 3 17 -0.5087131906393978 3 18 0.017664206168078067 3 19 -0.56016360244125 3 20 0.1311089098925109 3 21 -0.23416616063857276 3 22 0.11560506434199169 3 23 0.3995488929134865 3 24 0.251423011221668 3 25 0.12081643858642366 3 26 0.3571276644333281 3 27 -0.33764905999489514 3 28 0.7551217697292287 3 29 0.45514886209829797 3 30 0.6842204058611898 3 31 0.3358536957703464 3 32 0.35865705360659206 3 33 -0.17591672901448874 3 34 0.881304579070644 3 35 0.742051843248241 3 36 0.7771161374780036 3 37 0.27959380105569664 3 38 0.4111973495738823 3 39 -0.1601191762858818 3 40 -0.051495131761768675 3 41 0.6596698895242028 3 42 0.08872179573379668 3 43 0.007260073383456245 3 44 0.26730567655700455 3 45 0.1295815540179781 3 46 0.7963847320622044 3 47 0.0875690327198626 3 48 -0.007683330443391867 3 49 0.6452746722695331 3 50 1.1885093727588227 3 51 0.4624811017027106 3 52 1.2442842507208844 3 53 -0.9305764270396573 3 54 -0.1506462952515939 3 55 -0.5178431356252133 3 56 -0.9568611167216332 4 1 -0.6053879509047633 4 2 -0.5248320261086974 4 3 -0.46712812672596016 4 4 -0.8216493628344992 4 5 -0.34869532993069896 4 6 -0.46563574803783325 4 7 -0.011656542898343503 4 8 0.10757835597146936 4 9 0.00789401730876449 4 10 -0.45478938689091564 4 11 -1.1207423836292136 4 12 -1.3375896194289112 4 13 -0.9605196835792427 4 14 -0.9858738698335046 4 15 -0.7274093580013883 4 16 -0.7809277878261582 4 17 -1.2958508717988195 4 18 -0.9696546344116328 4 19 -0.7843483850499453 4 20 -1.3602860176437161 4 21 -0.9628799287545178 4 22 -0.7924565298609068 4 23 -0.8332409621589818 4 24 -0.15980887283630096 4 25 -0.3397813875005298 4 26 0.19363128762173432 4 27 -0.0650041324586681 4 28 -0.9328311288214977 4 29 -0.06070258717706167 4 30 0.7032421449916271 4 31 -0.16278760857748154 4 32 -0.6594588884223924 4 33 -0.3167681782898484 4 34 0.07378646312861292 4 35 0.39422575629171774 4 36 -0.5630106741161995 4 37 -0.6334496772051708 4 38 -0.109183085208727 4 39 -0.35305395889457714 4 40 0.01734544794837234 4 41 -0.04322866120043756 4 42 -0.11417675499084012 4 43 0.07428905889070236 4 44 -0.47182475822560477 4 45 0.04443662648174396 4 46 -0.9925645433001158 4 47 -0.945039662932313 4 48 -0.9460891275448411 4 49 -0.8153231538174239 4 50 -0.1448239605745094 4 51 -0.15798991279004326 4 52 -0.16966502464143574 4 53 0.5463257468733858 4 54 0.2039732699657968 4 55 -0.36385762837883817 4 56 0.5956751151624236 5 1 -0.20573120033267855 5 2 -0.12654827324828233 5 3 0.081613292038341 5 4 -0.37771343606104324 5 5 0.10828407510362581 5 6 -0.15694009586391644 5 7 0.14600936099181894 5 8 -0.0532912092459199 5 9 -0.05549271038002246 5 10 -0.32115094295498636 5 11 0.03863976740053232 5 12 0.25760488858024644 5 13 0.7187709342697275 5 14 0.028542606139041027 5 15 -0.28724917493502744 5 16 -0.21239231871860653 5 17 0.21387452865884882 5 18 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2.790862223921092 6 44 2.6570906227935644 6 45 3.0664095110072225 6 46 3.108884732062201 6 47 2.3133754843327665 6 48 2.581026346975964 6 49 2.058917145387813 6 50 1.8874341039416187 6 51 2.359658521057547 6 52 2.8430745733015286 6 53 2.692071422422707 6 54 2.252243489694642 6 55 2.101780520288763 6 56 1.6224399585471847 7 1 0.007398397856341887 7 2 0.09823950264306092 7 3 0.14624260726188254 7 4 -0.25830200425572514 7 5 -0.0927346006179377 7 6 0.030156678329632314 7 7 -0.02965560788197763 7 8 0.08864427462504665 7 9 0.01140032866921814 7 10 0.3039763915102114 7 11 -0.5575119020957899 7 12 0.1231396933340605 7 13 -0.19158560223281995 7 14 -0.5938682597353235 7 15 -0.18685302326553455 7 16 -0.07837986823756182 7 17 -0.3696002874135971 7 18 -0.6610992346921378 7 19 -0.5705802691079143 7 20 -0.09637764924727321 7 21 -0.25063121440201286 7 22 -0.921021279744032 7 23 -0.8753166984843688 7 24 -0.2902436554449963 7 25 0.03983525579072733 7 26 -0.3018105076096873 7 27 -0.1272995976293032 7 28 0.42212445790126907 7 29 0.23908703414131338 7 30 0.5934945994095742 7 31 0.6540660613617462 7 32 0.4554312471549764 7 33 1.5824838086199193 7 34 0.8386298478878444 7 35 1.578073348624585 7 36 1.2451940944672515 7 37 1.6532497150341925 7 38 1.0197322958104422 7 39 2.13490770543455 7 40 1.6366769112489834 7 41 1.482250534685491 7 42 1.1478615806800327 7 43 0.7693568475770043 7 44 1.2269830959118444 7 45 1.7008181131577587 7 46 0.3239384955030644 7 47 0.7327303230424427 7 48 0.3122091426748881 7 49 0.757841876570609 7 50 0.5648534587803304 7 51 1.0263251877242148 7 52 1.0473756485703447 7 53 0.9071251858635669 7 54 1.2092327370064702 7 55 1.574898799858655 7 56 1.342870066074065 8 1 -0.45346681844773684 8 2 -0.45127287545146544 8 3 -0.5283010286470926 8 4 -0.6163915266363205 8 5 -0.33504517826234803 8 6 0.07329246227561725 8 7 -0.13905795846962832 8 8 -0.0951516743621994 8 9 -0.049739956402049756 8 10 0.377869864878555 8 11 0.23643658502599152 8 12 0.49860856055087055 8 13 0.550349881638148 8 14 0.5985548460411181 8 15 0.7652600049915321 8 16 0.47463338507576225 8 17 0.6477790574226177 8 18 0.22599753950141377 8 19 0.7588770952331672 8 20 0.8692387548537539 8 21 1.4971807385862306 8 22 1.1231147542644706 8 23 1.0676690479522435 8 24 0.9306865771131427 8 25 0.8831710897492115 8 26 1.2221954938906876 8 27 0.8159362113229349 8 28 2.1129221573261248 8 29 1.284848474501402 8 30 2.1943948244658387 8 31 2.537161835305227 8 32 2.1299748830639516 8 33 2.2206433485048933 8 34 3.2042212457373083 8 35 3.294377424643592 8 36 3.902358385540019 8 37 3.680756591753372 8 38 4.031110140271558 8 39 4.0738924516210915 8 40 4.0337761860676835 8 41 4.721685393400172 8 42 5.208876834493491 8 43 5.064430616019113 8 44 4.505677769580263 8 45 5.390240468746661 8 46 4.907334344465305 8 47 5.27361554434777 8 48 4.703556979634126 8 49 4.733210718781162 8 50 4.9947109231464175 8 51 6.320523737361626 8 52 6.410466421263521 8 53 6.868115433425462 8 54 6.972173472190267 8 55 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1.865018694073111 120 44 2.023052802993785 120 45 1.8005585655122331 120 46 0.9939422033265721 120 47 1.1910173085819302 120 48 1.3515120718554563 120 49 1.6499442124994168 120 50 1.7287392578162937 120 51 2.638486848829146 120 52 2.7507497679622617 120 53 1.8592942626155136 120 54 3.6126439346334642 120 55 3.6071568643747867 120 56 1.6480239407496295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 35.0 5 1.0 6 12.0 7 27.0 8 50.0 9 39.0 10 71.0 11 97.0 12 129.0 13 200.0 14 274.0 15 328.0 16 449.0 17 536.0 18 676.0 19 791.0 20 812.0 21 977.0 22 1007.0 23 1114.0 24 1343.0 25 1524.0 26 1652.0 27 1829.0 28 2024.0 29 2274.0 30 2566.0 31 3000.0 32 3597.0 33 4642.0 34 6073.0 35 8098.0 36 11228.0 37 15339.0 38 16679.0 39 8975.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.90218141934436 0.9607182028679369 0.07921355161067554 0.05788682617703213 8 99.3864407468357 0.31084293289449627 0.03352227707685744 0.2691940431929461 9 98.74070764106105 0.7312020148677743 0.21022057927448512 0.3178697647966852 10 43.35317057318113 20.420847381890564 26.35373928585937 9.872242759068936 11 27.57240931063899 41.78616403298533 14.805825242718445 15.83560141365723 12 25.509810293699474 12.854937644717065 19.680505341836945 41.95474671974652 13 20.06743307470447 12.457854328309704 23.14051265385709 44.33419994312873 14 19.547467197465167 16.090506560506967 44.873461429093716 19.48856481293415 15 41.95271560303855 16.04379087622375 21.908640370475688 20.094853150262015 16 43.85282528334078 15.57054068326766 18.592842344721127 21.983791688670433 17 23.485802494211317 15.478124873055204 39.608806922045744 21.427265710687738 18 21.936060446033228 16.496729902100174 41.32713165698501 20.240077994881585 19 44.92525490514685 16.611487996100255 18.49941097615469 19.963846122598206 20 22.301887942153208 39.17353021824571 18.301562960179552 20.22301887942153 21 21.430312385749687 16.84506641751635 41.46220091806475 20.262420278669214 22 23.477678027379454 14.288906040541088 19.375837835642034 42.85757809643742 23 21.99293171385628 17.72555551041963 18.762440589836295 41.5190721858878 24 20.81027005616437 18.359553528808362 39.85131168686078 20.97886472816648 25 44.03198114453486 16.352239594444953 18.629931019068806 20.985848241951377 26 21.913018731768798 16.685808372887212 18.8405207899257 42.56065210541829 27 20.690916852151112 15.989267311034547 19.285300484800437 44.034515352013905 28 21.535692861427712 37.443697701137744 20.057344463310727 20.963264974123817 29 43.38081188320277 16.91423339098586 19.163699257299825 20.541255468511547 30 21.610199696534586 16.29446328374016 41.80286968299066 20.29246733673459 31 22.849807445442877 36.72290482303319 21.05874442203069 19.368543309493244 32 44.14991697313597 15.909577123297439 21.062336365766445 18.878169537800144 33 22.415268918010067 16.301180019157478 22.317443495628424 38.96610756720403 34 20.726312785388128 38.279721135029355 24.009295499021526 16.984670580560994 35 20.79756102534821 18.790912984073252 44.82533597079756 15.586190019780982 36 22.160006527548294 38.925606348040716 21.781612712400303 17.13277441201069 37 21.221641912844785 18.259343887710138 43.23996246123715 17.279051738207933 38 42.21133731312822 18.749936221235778 22.266442165416603 16.772284300219397 39 21.961228677303772 17.357261711532377 21.661103114568338 39.02040649659552 40 22.062533835892093 17.5486981480914 42.56836127028877 17.82040674572774 41 22.185217346886652 16.72370639015171 42.79715993257394 18.2939163303877 42 40.176564351984304 16.71945886295827 24.15804961785262 18.945927167204808 43 19.5477731096732 16.23478180870312 44.66456090854263 19.55288417308105 44 19.031265650711884 16.510798352395263 23.878003659072558 40.579932337820296 45 19.526784527449156 16.886675732880704 44.437059683636846 19.149480056033294 46 18.895616421543558 15.797655818520262 24.858346799762717 40.448380960173466 47 19.385833862022388 37.28383439412234 25.234840267687208 18.095491476168064 48 20.498802137723448 37.06308741323177 23.91322153285419 18.524888916190594 49 19.517936646026303 36.96126093853948 22.977329717005272 20.543472698428943 50 39.12233089251882 17.063853755952685 23.619232935634184 20.19458241589431 51 19.8282040256363 37.91592440158077 24.592010156234004 17.66386141654893 52 20.472908623567605 18.008008110516023 42.547439350339474 18.9716439155769 53 20.71659703448841 17.011755550094207 43.075694273777344 19.195953141640043 54 20.903068700084614 37.18245041995997 22.146439110964362 19.768041768991065 55 19.850628528107038 37.57440399963119 21.921134321630177 20.653833150631602 56 38.981818181818184 17.45966709346991 21.58361075544174 21.974903969270166 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.5 7 5.0 8 17.5 9 30.0 10 47.5 11 65.0 12 65.5 13 83.5 14 101.0 15 220.0 16 339.0 17 396.0 18 453.0 19 468.5 20 484.0 21 629.0 22 816.0 23 858.0 24 1000.5 25 1143.0 26 1317.5 27 1492.0 28 1517.0 29 1574.5 30 1607.0 31 1451.5 32 1296.0 33 1337.0 34 1378.0 35 1242.0 36 1106.0 37 977.0 38 867.5 39 887.0 40 1099.0 41 1311.0 42 2091.5 43 2872.0 44 11584.0 45 20296.0 46 12235.5 47 3541.5 48 2908.0 49 3410.5 50 3913.0 51 3639.0 52 3365.0 53 3583.5 54 3867.5 55 3933.0 56 4655.5 57 5378.0 58 5500.5 59 5623.0 60 5574.5 61 5526.0 62 5331.0 63 4591.0 64 4046.0 65 3515.0 66 2984.0 67 2707.5 68 2431.0 69 2166.5 70 1902.0 71 1740.5 72 1387.0 73 1195.0 74 931.0 75 667.0 76 537.5 77 408.0 78 337.0 79 211.5 80 157.0 81 115.0 82 73.0 83 51.0 84 29.0 85 21.0 86 13.0 87 7.5 88 2.0 89 2.0 90 1.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.02640451720355852 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.00101555835398302 21 0.0 22 0.0 23 0.0 24 0.00710890847788114 25 0.0355445423894057 26 0.08022910996465857 27 0.07819799325669254 28 0.11678921070804728 29 0.18076938700897754 30 0.2731851972214323 31 0.31888532315066825 32 0.3117764146727871 33 0.3391964902303286 34 0.3615387740179551 35 0.4001299914693098 36 0.42856562538083437 37 0.44278344233659667 38 0.4803591014339684 39 0.5169192021773571 40 0.5778527034163383 41 0.5920705203721005 42 0.6093350123898119 43 0.6509729049031158 44 0.6387862046553194 45 0.6794085388146404 46 0.7047974976642158 47 0.7535442986554007 48 0.8063533330625178 49 0.7748710240890442 50 0.8358045253280254 51 0.8063533330625178 52 0.8297111752041273 53 0.8246333834342121 54 1.578177682089613 55 0.8723646260714141 56 0.8561156924076857 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 98468.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.04151602551082 #Duplication Level Percentage of deduplicated Percentage of total 1 69.83293556085918 23.772189950034527 2 12.312052505966587 8.382418653775845 3 5.757756563245824 5.880082869561685 4 3.2338902147971362 4.403461022870374 5 2.022673031026253 3.4427428200024375 6 1.3931980906921242 2.8455945078604215 7 1.0292362768496421 2.452573424868993 8 0.6921241050119332 1.884876304992485 9 0.513126491646778 1.5720843319657147 >10 2.93854415274463 18.202867936791648 >50 0.1909307875894988 4.296827395702157 >100 0.07756563245823389 5.125523012552302 >500 0.0029832935560859188 0.5524637445667628 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0029832935560859188 17.186294024454646 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 16923 17.186294024454646 No Hit ATCAACGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 544 0.5524637445667628 No Hit ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 449 0.4559857009383759 No Hit ATCAACGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 428 0.4346589755047325 No Hit ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT 358 0.3635698907259211 No Hit ATCAACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 318 0.3229475565666003 No Hit ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA 307 0.3117764146727871 Illumina Single End Adapter 1 (95% over 21bp) ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 278 0.28232522240727953 No Hit ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 240 0.2437340049559248 No Hit ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 209 0.21225169598245117 No Hit ATCAACGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 195 0.19803387902668887 No Hit ATCAACGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC 171 0.1736604785310964 No Hit ATCAACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 165 0.16756712840719828 No Hit ATCAACGGGGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACT 154 0.15639598651338507 No Hit ATCAACAGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 151 0.153349311451436 No Hit ATCAACGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 147 0.14928707803550392 No Hit ATCAACGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG 144 0.14624040297355487 No Hit ATCAACGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 139 0.14116261120363977 No Hit ATCAACGGGTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACG 138 0.14014705284965673 No Hit ATCAACGGGCAGATTCAGAACGTGGGAGGTTTTGTGTCTGGCTTATTTCCTTTTGC 133 0.13506926107974165 No Hit ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTNAG 130 0.13202258601779257 No Hit ATCAACGGGGGCCATCTAACCCATCCCTGGCCTGGCTGCGGGGCGCTTCCCGCAAC 126 0.1279603526018605 No Hit ATCAACGGGTACCTGGTTGATCCTGCCAGTAGCATTGCTTGTCTCAAAGATTAAGC 120 0.1218670024779624 No Hit ATCAACGGGGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC 110 0.11171141893813218 No Hit ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 110 0.11171141893813218 No Hit ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 110 0.11171141893813218 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 110 0.11171141893813218 No Hit ATCAACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 107 0.10866474387618312 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.00203111670796604 0.0 0.0 0.0 0.0 10 0.00406223341593208 0.0 0.0 0.0 0.0 11 0.00812446683186416 0.0 0.0 0.0 0.0 12 0.00914002518584718 0.0 0.0 0.0 0.0 13 0.010155583539830199 0.0 0.0 0.0 0.0 14 0.014217816955762278 0.0 0.0 0.0 0.0 15 0.01726449201771134 0.0 0.0 0.0 0.0 16 0.01828005037169436 0.0 0.0 0.0 0.0 17 0.020311167079660398 0.0 0.0 0.0 0.0 18 0.023357842141609457 0.0 0.0 0.0 0.0 19 0.028435633911524556 0.0 0.0 0.0 0.0 20 0.029451192265507575 0.0 0.0 0.0 0.0 21 0.031482308973473615 0.0 0.0 0.0 0.0 22 0.03656010074338872 0.0 0.0 0.0 0.0 23 0.03757565909737173 0.0 0.0 0.0 0.0 24 0.03757565909737173 0.0 0.0 0.0 0.0 25 0.038591217451354756 0.0 0.0 0.0 0.0 26 0.04163789251330381 0.0 0.0 0.0 0.0 27 0.04366900922126985 0.0 0.0 0.0 0.0 28 0.053824592761100055 0.0 0.0 0.0 0.0 29 0.055855709469066095 0.0 0.0 0.0 0.0 30 0.06296461794694723 0.0 0.0 0.0 0.0 31 0.06702685136287931 0.0 0.0 0.0 0.0 32 0.06804240971686233 0.0 0.0 0.0 0.0 33 0.06905796807084535 0.0 0.0 0.0 0.0 34 0.07210464313279441 0.0 0.0 0.0 0.0 35 0.07413575984076046 0.0 0.0 0.0 0.0 36 0.07819799325669254 0.0 0.0 0.0 0.0 37 0.07921355161067554 0.0 0.0 0.0 0.0 38 0.08530690173457367 0.0 0.0 0.0 0.0 39 0.09140025185847178 0.0 0.0 0.0 0.0 40 0.09546248527440386 0.0 0.0 0.0 0.0 41 0.09850916033635293 0.0 0.0 0.0 0.0 42 0.10054027704431896 0.0 0.0 0.0 0.0 43 0.10358695210626803 0.0 0.0 0.0 0.0 44 0.10460251046025104 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGCCT 15 0.008029931 50.585266 50 CACTCTA 20 3.9324348E-4 50.585262 49 ATCTCGG 15 0.00831174 50.14607 47 TAGAATG 15 0.00831174 50.14607 47 GACGTCG 15 0.00831174 50.14607 46 ACGCTCG 15 0.00831174 50.14607 45 ACGTCGC 15 0.00831174 50.14607 47 GATCTCG 15 0.00831174 50.14607 46 CGTTTCA 15 0.00831174 50.14607 45 ATCCGTT 15 0.008328543 50.120472 42 GAAACGC 15 0.008328543 50.120472 42 GTGACGT 15 0.008328543 50.120472 44 CTATACA 20 4.1158707E-4 50.12047 42 TATACAA 20 4.1158707E-4 50.12047 43 ATACAAG 20 4.1158707E-4 50.12047 44 AATCCGT 15 0.008345372 50.0949 41 GCTATAC 20 4.1262573E-4 50.0949 41 TCAATCC 15 0.008345372 50.0949 39 TGCTATA 20 4.1262573E-4 50.0949 40 CTGGGCT 15 0.008345372 50.0949 39 >>END_MODULE