FastQCFastQC Report
Fri 17 Jun 2016
SRR1526670_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526670_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73355
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT17082.3284029718492265No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.3339922295685366No Hit
GCCCTCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT1700.2317497103128621No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGA1280.17449389952968442No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.1608615636289278No Hit
GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT1070.14586599413809556No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC920.12541749028696067No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.12405425669688502No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC890.1213277895167337No Hit
GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT740.1008792856655988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGC258.1292965E-658.5456
AGGTCCA150.004501356358.53999758
TCGGGTC501.8189894E-1258.2187925
CCTCGGA351.5656042E-858.2187923
TCGTGTG150.004600587358.2187925
CGGGATG1200.058.2187926
CGGGATC750.058.2187926
CGGGATA351.5656042E-858.2187926
CGGGACA1250.058.2187926
GGGATAC150.004600587358.2187927
CGGGTCA201.9610125E-458.218796
CGGGTAT201.9610125E-458.218796
TCGGGAT3050.058.218795
GGAGAGT150.004650809358.0595058
CTCTAAA150.004650809358.05950555
TACGCCA201.9945248E-458.0198253
GTCAAGA150.004663428358.01981753
GATTACG258.606528E-657.9801950
AAAGCTT258.606528E-657.9801947
GTAAAAT258.606528E-657.9801945
Produced by FastQC (version 0.11.5)