FastQCFastQC Report
Fri 17 Jun 2016
SRR1526669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101266
Sequences flagged as poor quality0
Sequence length64
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT47964.736041711927004No Hit
GCCCTCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT5160.5095491082890604No Hit
GCCCTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG5040.49769912902652425No Hit
GCCCTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG4860.4799241601327197No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.3505618865166986No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2940.2903244919321391No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2520.24884956451326212No Hit
GCCCTCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG2000.19749965437560485No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.1965121561037268No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC1870.18466217684119052No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGA1860.18367467856931252No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1810.17873718720992238No Hit
GCCCTCGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG1790.17676219066616633No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1620.15997472004423993No Hit
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1580.15602472695672784No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCAT1530.1510872355973377No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTC1500.14812474078170365No Hit
GCCCTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1400.1382497580629234No Hit
GCCCTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1330.13133727015977723No Hit
GCCCTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1310.12936227361602118No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1300.12837477534414315No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1290.1273872770722651No Hit
GCCCTCGGGGGGTGGCCACAAGCTTGGCCTAGGACTGGAATTTCAAGCATAAATGAATATTGTA1290.1273872770722651No Hit
GCCCTCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC1260.12442478225663106No Hit
GCCCTCGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1230.12146228744099698No Hit
GCCCTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1210.11948729089724093No Hit
GCCCTCGGGGGGACTGGAGAGATGGCCCAGTGGTTAAGAGCACTAAGTGCTCTTCCGAAGGTCC1180.11652479608160685No Hit
GCCCTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAAGGTCC1170.11553729780972882No Hit
GCCCTCGGGCTTCCCCATCTCTCCTTGCAGCCTCTTCAACCAGTTGATTACCCAGGTGATTGGT1160.1145497995378508No Hit
GCCCTCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1160.1145497995378508No Hit
GCCCTCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT1140.11257480299409477No Hit
GCCCTCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT1130.11158730472221674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATATT150.004626210358.15342753
ATCTACG150.004626210358.15342757
AAGAATC150.004626210358.15342753
TCTAGCT150.004626210358.15342752
AATGCCA150.004626210358.15342757
AATCTAC150.004626210358.15342756
TACTCTG150.004626210358.15342753
AGCTACT150.004626210358.15342752
TAGCTGC201.97557E-458.15342754
TATAAAT150.004626210358.15342754
ACTATTA150.004635317358.12456547
GAATCTA150.004635317358.12456555
AAGCGCA550.058.12456551
GTAATTT150.004635317358.12456547
CTTGCGA201.9804282E-458.12456548
ATACAGC150.004635317358.12456548
TCACGAT150.00464443758.09573440
TTGATTA201.9852958E-458.09573444
AGCGTCA150.00464443758.09573439
CTCACGA150.00464443758.09573439
Produced by FastQC (version 0.11.5)