FastQCFastQC Report
Fri 17 Jun 2016
SRR1526668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101755
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT46034.523610633384109No Hit
GCCCTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG4790.4707385386467496No Hit
GCCCTCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT4440.43634219448675743No Hit
GCCCTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4430.4353594417964719No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.3302049039359245No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2800.2751707532799371No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2390.23487789297823203No Hit
GCCCTCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTG2050.20146430150852537No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1960.192619527295956No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.18279200039310106No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCCGAAGGTC1550.1523266669942509No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC1520.1493784089233944No Hit
GCCCTCGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG1500.14741290354282346No Hit
GCCCTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1480.14544739816225247No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1460.1434818927816815No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGA1410.13856812933025403No Hit
GCCCTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1310.12874060242739915No Hit
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1280.12579234435654268No Hit
GCCCTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1240.12186133359540072No Hit
GCCCTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCCAAAGGTCC1200.11793032283425875No Hit
GCCCTCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC1190.11694757014397326No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1150.11301655938283131No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTGAAGGTCAT1150.11301655938283131No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1110.10908554862168936No Hit
GCCCTCGGGGGGTGGCCACAAGCTTGGCCTAGGACTGGAATTTCAAGCATAAATGAATATTGTA1060.10417178517026189No Hit
GCCCTCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1040.10220627978969092No Hit
GCCCTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1020.10024077440911995No Hit
GCCCTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1020.10024077440911995No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCG150.004515788558.50921643
CCAACGG150.004515788558.50921642
ATCCGAG150.00453364458.4509740
TAATATC150.004587526458.27692420
TGCATAC150.004650990558.07517252
CTTGCGA150.004650990558.07517248
CATACAG150.004650990558.07517254
CGCACCT150.004650990558.07517249
CCTTGCG150.004650990558.07517247
ATACAGT150.004650990558.07517255
AACACTA258.54692E-658.07517248
GATTTGC150.00467838857.98913637
TATCTGG202.003442E-457.98913636
GATGCCT150.00467838857.98913637
ATTTCCG202.003442E-457.98913637
CCAGTTA150.004696720357.9319235
AAAATCT150.004696720357.9319235
GTAGGAT150.004724318657.84630633
CCCGTCA150.004724318657.84630634
ATCCAGT150.004724318657.84630633