FastQCFastQC Report
Fri 17 Jun 2016
SRR1526667_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526667_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94945
Sequences flagged as poor quality0
Sequence length56
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG49795.244088682921691No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC4900.5160882616251514No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4790.5045026067723419No Hit
GCCCTCGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC4260.44868081520880504No Hit
GCCCTCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC4100.4318289536047185No Hit
GCCCTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC3910.41181736794986573No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3900.4107641265996103No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.21275475275159303No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT1840.19379640844699564No Hit
GCCCTCGGGGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCC1810.1906366843962294No Hit
GCCCTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1790.18853020169571857No Hit
GCCCTCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1770.18642371899520774No Hit
GCCCTCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1490.15693296118805625No Hit
GCCCTCGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT1380.14534730633524673No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1280.1348148928326926No Hit
GCCCTCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1260.1327084101321818No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAA1220.12849544473116015No Hit
GCCCTCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTG1220.12849544473116015No Hit
GCCCTCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1210.12744220338090473No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC1200.12638896203064934No Hit
GCCCTCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1190.12533572068039392No Hit
GCCCTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1170.1232292379798831No Hit
GCCCTCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA1120.11796303122860603No Hit
GCCCTCGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCTTCC1100.11585654852809522No Hit
GCCCTCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC1080.11375006582758439No Hit
GCCCTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1050.11059034177681815No Hit
GCCCTCGGGGAGGCTCCCCGCGGGTCTTCAACTTTGATTTCCGCCGGCAGGGCCCT1010.10637737637579651No Hit
GCCCTCGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG1000.1053241350255411No Hit
GCCCTCGGGGGGTGGCCACAAGCTTGGCCTAGGACTGGAATTTCAAGCATAAATGA990.10427089367528569No Hit
GCCCTCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG990.10427089367528569No Hit
GCCCTCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG950.10005792827426405No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAG150.00789000450.80903250
ATCTGGA150.00789000450.80903250
GTGTTAG251.8908393E-550.7817249
TGTGTTA251.8908393E-550.7817248
AGCGCGA204.0132037E-450.3754747
ACTACTC150.00816207450.37546547
CAATTAT204.0450195E-450.29499444
AGTAAAA150.00821385350.2949944
GACTACT150.00821385350.2949946
CGCATCT150.00823116850.26822742
CGTGCCC150.00828327250.18809541
AAAAGTA150.00828327250.18809541
TTACTTG150.00828327250.18809541
ACCAACT150.00828327250.18809541
GGCAAAC252.0400224E-550.1348239
AACCAGT150.00831814650.13481538
GCCGCAT150.00831814650.13481540
ACCAGTT150.00831814650.13481539
GAACATG150.00831814650.13481540
GTACGTA150.00831814650.13481539
Produced by FastQC (version 0.11.5)