FastQCFastQC Report
Fri 17 Jun 2016
SRR1526666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39583
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT19414.903620241012556No Hit
AAGCGGGGGTAATTGAAGTCAATAACCTGTTCCCAAGTGACTGTCATTTAGCGATATTTGATCC1020.2576863805168886No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCCT990.2501073693252154No Hit
AAGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG990.2501073693252154No Hit
AAGCGGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT810.20463330217517622No Hit
AAGCGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1768435944723745No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.17431725740848344No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCACGCAT660.16673824621681024No Hit
AAGCGGGGGGTGTGGTTTAGTAGAGATATTAAAATTATTAAAAATACTATGTTGATTTCAGAAG610.15410656089735492No Hit
AAGCGGGGGAGCACTGTCAAGAACAGTTTCTTTCCATCAGTATTGCTAAAAGTTATTTCAAGTC540.1364222014501175No Hit
AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA440.11115883081120684No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCCTGCAT420.1061061566834247No Hit
AAGCGGGGGCATGGTAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAG400.10105348255564257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAGCT150.004476534758.57051557
CGGGGTA201.9410432E-458.2716874
CGGGGAC150.004568291458.2716874
GGGTAAT150.004568291458.2716877
CGATATT150.004614691358.1234152
GCGATAT150.004614691358.1234151
ATATTTG150.004614691358.1234154
GCTTAAG150.004614691358.1234150
GTCATTT150.004638022358.04955343
TGTCATT150.004638022358.04955342
GGCGACT150.004638022358.04955346
CCCTACC150.004638022358.04955342
TCCCAAG150.004661441357.97588731
CCGTGAT150.004661441357.97588737
GGACTAT258.536896E-657.9758878
GGATTTC150.004661441357.9758878
ATGCGTC150.004661441357.97588736
CCCAAGT150.004661441357.97588732
GTCCATG150.004661441357.97588732
GACTGTC150.004661441357.97588739
Produced by FastQC (version 0.11.5)