FastQCFastQC Report
Fri 17 Jun 2016
SRR1526665_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526665_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62839
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT53908.577475771415841No Hit
AAGCGGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT3480.5537962093604291No Hit
AAGCGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG2990.4758191568930123No Hit
AAGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG1880.2991772625280479No Hit
AAGCGGGGGCTGAGATTAAATGTCTGCACTTACCAGGCCCAGTTCAAAATGGTATTTTGTTTGT1630.2593930520854883No Hit
AAGCGGGGGTGGCATTGAGTACTGGGTCCTGAGTCGTGGGGCTTTCTTCTCACAGGCACAAAAA1420.2259743153137383No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1410.22438294689603588No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1370.21801747322522635No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.2084692627190121No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1260.20051242063050018No Hit
AAGCGGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG1220.19414694695969065No Hit
AAGCGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.19255557854198826No Hit
AAGCGGGGGTAATTGAAGTCAATAACCTGTTCCCAAGTGACTGTCATTTAGCGATATTTGATCC1180.18778147328888112No Hit
AAGCGGGGATTCTGACTATGATTAAGGATAGCAGGAAGTCTATAAGCTATAACATAAAATAGTG1140.18141599961807156No Hit
AAGCGGGGGAGCACTGTCAAGAACAGTTTCTTTCCATCAGTATTGCTAAAAGTTATTTCAAGTC1070.1702764206941549No Hit
AAGCGGGGGGGAGCCGCTGCTGGCTCTGTGTCTCTGTCCTCAGCGTTCTCGTCCTCGTCCTCGT1040.16550231544104774No Hit
AAGCGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1020.16231957860564297No Hit
AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC1000.15913684177023824No Hit
AAGCGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA820.13049221025159535No Hit
AAGCGGGGGAGACTCTGAACTTACCGAGTTTGCCCGCCGTGATTAGCTCGCGGAAGGGAGAAGC810.12890084183389297No Hit
AAGCGGGGGGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCCTGGAGGAGTAC800.1273094734161906No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC780.12412673658078581No Hit
AAGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG780.12412673658078581No Hit
AAGCGGGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC780.12412673658078581No Hit
AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA740.1177612629099763No Hit
AAGCGGGGGCATGGTAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAG740.1177612629099763No Hit
AAGCGGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT730.11616989449227391No Hit
AAGCGGGGGGTGTGGTTTAGTAGAGATATTAAAATTATTAAAAATACTATGTTGATTTCAGAAG720.11457852607457153No Hit
AAGCGGGGGCTTGCATCCTTCCGGCTCCGTCGTGGAAGGAGGACTGCGCCTCGTCTTCCTGAAG710.11298715765686915No Hit
AAGCGGGGGACTCGAAGTGGAGCGCAGTCGGGGCCGGGCGTGTAGTCGGATCAGCCGCCGCCAT710.11298715765686915No Hit
AAGCGGGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT670.1066216839860596No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT660.10503031556835722No Hit
AAGCGGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGA630.10025621031525009No Hit
AAGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA630.10025621031525009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAATCC201.950963E-458.2658657
TTTCGAC150.004582745558.26585857
TGAAATT150.004582745558.26585857
TTCGACG150.004582745558.26585858
TCAAAAT201.9587336E-458.21909744
TATCATA201.9587336E-458.21909747
CATGGTC150.00459735358.21909753
AGCGTTC150.00459735358.21909742
ACTATGT201.9587336E-458.21909746
ATCTCGC150.00459735358.21909741
GTACACC201.9587336E-458.21909749
GTTTCGA150.00459735358.21909756
CCCTACT201.9587336E-458.21909755
AACTTCG150.00459735358.21909753
TTGTACA201.9587336E-458.21909747
CTAAGGT150.00459735358.21909753
TTATCAT201.9587336E-458.21909746
CCATGAA201.9587336E-458.21909754
TGTTGAT201.9587336E-458.21909750
CAGATTT150.00459735358.21909749
Produced by FastQC (version 0.11.5)