FastQCFastQC Report
Fri 17 Jun 2016
SRR1526664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62385
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT49307.902540674841709No Hit
AAGCGGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATT3590.5754588442734632No Hit
AAGCGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTG2820.45203173839865357No Hit
AAGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATG1960.31417808768133365No Hit
AAGCGGGGGTGGCATTGAGTACTGGGTCCTGAGTCGTGGGGCTTTCTTCTCACAGGCACAAAAA1640.2628837060190751No Hit
AAGCGGGGGCTGAGATTAAATGTCTGCACTTACCAGGCCCAGTTCAAAATGGTATTTTGTTTGT1420.22761881862627234No Hit
AAGCGGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG1300.20838342550292538No Hit
AAGCGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.20357457722208866No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1190.190750981806524No Hit
AAGCGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1170.18754508295263284No Hit
AAGCGGGGATTCTGACTATGATTAAGGATAGCAGGAAGTCTATAAGCTATAACATAAAATAGTG1040.1667067404023403No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1030.16510379097539474No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.160294942694558No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC990.1586919932676124No Hit
AAGCGGGGGTAATTGAAGTCAATAACCTGTTCCCAAGTGACTGTCATTTAGCGATATTTGATCC970.15548609441372124No Hit
AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA940.15067724613288452No Hit
AAGCGGGGGGGAGCCGCTGCTGGCTCTGTGTCTCTGTCCTCAGCGTTCTCGTCCTCGTCCTCGT880.14105954957121103No Hit
AAGCGGGGGGTGTGGTTTAGTAGAGATATTAAAATTATTAAAAATACTATGTTGATTTCAGAAG870.13945660014426545No Hit
AAGCGGGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTT850.1362507012903743No Hit
AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC840.1346477518634287No Hit
AAGCGGGGGGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCCTGGAGGAGTAC810.12983890358259198No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC800.12823595415564637No Hit
AAGCGGGGGAGCACTGTCAAGAACAGTTTCTTTCCATCAGTATTGCTAAAAGTTATTTCAAGTC780.12503005530175523No Hit
AAGCGGGGGATCATTGAGATGGACTCAAAGCGTGTGCCTCGGGACAAGCTGGCCTGCATCACCA780.12503005530175523No Hit
AAGCGGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTAT720.11541235874008177No Hit
AAGCGGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG720.11541235874008177No Hit
AAGCGGGGGAGACTCTGAACTTACCGAGTTTGCCCGCCGTGATTAGCTCGCGGAAGGGAGAAGC710.11380940931313617No Hit
AAGCGGGGGCATGGTAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAG670.10739761160535384No Hit
AAGCGGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGA660.10579466217840827No Hit
AAGCGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT660.10579466217840827No Hit
AAGCGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCA640.10258876332451712No Hit
AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT640.10258876332451712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAC150.00452123558.46422623
AGATCCC201.918277E-458.46422622
ACACGAT150.00452123558.46422645
ACTGCGC150.00452123558.46422643
CGTGCAC150.00452123558.46422640
CACATAG150.00452123558.46422623
GCCGGTC150.00452123558.46422623
GTGATTA201.918277E-458.46422639
GATCACC150.00452123558.46422623
GTCTAAA150.00452123558.46422642
CTAAGGT150.00452123558.46422625
TGAGTGG150.00452123558.46422639
AGTATTG150.00452123558.46422639
TATTGCT150.00452123558.46422641
TGATTAG201.918277E-458.46422640
TCTAAAA150.00452123558.46422643
TAAGGTG150.00452123558.46422626
GTCCCCC150.00452123558.46422622
CACACGA150.00452123558.46422644
ATTGCTA150.00452123558.46422642
Produced by FastQC (version 0.11.5)