SRR1526663_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526663_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	57432
Sequences flagged as poor quality	0
Sequence length	56
%GC	57

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	5594	9.740214514556344	No Hit
AAGCGGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC	308	0.5362863908622371	No Hit
AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	278	0.4840507034405906	No Hit
AAGCGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC	212	0.3691321911129684	No Hit
AAGCGGGGGCTGAGATTAAATGTCTGCACTTACCAGGCCCAGTTCAAAATGGTATT	202	0.3517202953057529	No Hit
AAGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC	189	0.32908483075637274	No Hit
AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	136	0.23680178297813068	No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	124	0.21590750800947206	No Hit
AAGCGGGGATTCTGACTATGATTAAGGATAGCAGGAAGTCTATAAGCTATAACATA	122	0.21242512884802897	No Hit
AAGCGGGGGTAATTGAAGTCAATAACCTGTTCCCAAGTGACTGTCATTTAGCGATA	120	0.20894274968658588	No Hit
AAGCGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	120	0.20894274968658588	No Hit
AAGCGGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT	114	0.19849561220225656	No Hit
AAGCGGGGGTGGCATTGAGTACTGGGTCCTGAGTCGTGGGGCTTTCTTCTCACAGG	110	0.19153085387937038	No Hit
AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	98	0.1706365789107118	No Hit
AAGCGGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	95	0.16541301016854715	No Hit
AAGCGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT	94	0.1636718205878256	No Hit
AAGCGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	91	0.15844825184566097	No Hit
AAGCGGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	90	0.1567070622649394	No Hit
AAGCGGGGGTGGATCTGAAAAATGATCAGGCATCCAGTGAGCTGCTTATCATACCC	87	0.15148349352277476	No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA	87	0.15148349352277476	Illumina Single End Adapter 1 (95% over 21bp)
AAGCGGGGGGTGTGTGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG	86	0.1497423039420532	No Hit
AAGCGGGGGGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGTGAG	81	0.14103635603844547	No Hit
AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	80	0.1392951664577239	No Hit
AAGCGGGGGAGCACTGTCAAGAACAGTTTCTTTCCATCAGTATTGCTAAAAGTTAT	78	0.13581278729628082	No Hit
AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT	76	0.13233040813483773	No Hit
AAGCGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	73	0.1271068393926731	No Hit
AAGCGGGGGGTGTGGTTTAGTAGAGATATTAAAATTATTAAAAATACTATGTTGAT	69	0.12014208106978688	No Hit
AAGCGGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC	68	0.11840089148906534	No Hit
AAGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	66	0.11491851232762224	No Hit
AAGCGGGGGGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCCTGG	66	0.11491851232762224	No Hit
AAGCGGGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC	63	0.10969494358545757	No Hit
AAGCGGGGGAGACTCTGAACTTACCGAGTTTGCCCGCCGTGATTAGCTCGCGGAAG	63	0.10969494358545757	No Hit
AAGCGGGGGGGAGCCGCTGCTGGCTCTGTGTCTCTGTCCTCAGCGTTCTCGTCCTC	63	0.10969494358545757	No Hit
AAGCGGGGGCATGGTAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCG	63	0.10969494358545757	No Hit
AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA	62	0.10795375400473603	No Hit
AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG	62	0.10795375400473603	No Hit
AAGCGGGGGGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGA	61	0.10621256442401449	No Hit
AAGCGGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT	59	0.1027301852625714	No Hit
AAGCGGGGGACTCGAAGTGGAGCGCAGTCGGGGCCGGGCGTGTAGTCGGATCAGCC	59	0.1027301852625714	No Hit
AAGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	58	0.10098899568184985	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACAT	20	3.8594365E-4	50.740448	49
TAACATA	20	3.8594365E-4	50.740448	50
TCATACC	20	3.8594365E-4	50.740448	49
ATCATAC	25	1.8902801E-5	50.740444	48
TCACAGG	25	1.8902801E-5	50.740444	50
TGTTGAT	15	0.007916057	50.74044	50
TCGCATC	15	0.007916057	50.74044	50
ATGTTGA	15	0.007916057	50.74044	49
CTATAAC	20	3.9970138E-4	50.38217	47
CTATGTT	15	0.008141211	50.382168	47
TACTCGC	15	0.008141211	50.382168	47
ATAAGCT	20	4.0144814E-4	50.33774	42
TAAGCTA	20	4.0144814E-4	50.33774	43
TTATCAT	25	1.9817211E-5	50.33774	46
AAATACT	15	0.008169687	50.33774	42
GTTCAAA	15	0.008169687	50.33774	42
CTGGACA	15	0.008169687	50.33774	43
CTTATCA	25	1.9817211E-5	50.33774	45
TGGACAA	15	0.008169687	50.33774	44
TTTGGGT	15	0.008169687	50.33774	44

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content