##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526663_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57432 Sequences flagged as poor quality 0 Sequence length 56 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.52573478200306 39.0 38.0 40.0 36.0 40.0 2 38.48868226772531 39.0 38.0 40.0 36.0 40.0 3 38.04469633653712 39.0 38.0 40.0 35.0 40.0 4 37.638024098063795 39.0 38.0 40.0 33.0 40.0 5 38.02300111436133 39.0 38.0 40.0 35.0 40.0 6 38.14324766680596 39.0 38.0 40.0 35.0 40.0 7 38.58178367460649 39.0 38.0 40.0 36.0 40.0 8 38.00832288619585 39.0 38.0 40.0 35.0 40.0 9 37.930073826438225 39.0 38.0 40.0 35.0 40.0 10 37.17025351720295 39.0 35.0 40.0 32.0 40.0 11 37.18480986209779 39.0 36.0 40.0 32.0 40.0 12 37.389486697311604 39.0 37.0 40.0 33.0 40.0 13 37.29998955286251 39.0 37.0 40.0 33.0 40.0 14 37.51889190695083 39.0 37.0 40.0 33.0 40.0 15 37.4922865301574 39.0 37.0 40.0 33.0 40.0 16 37.34250940242374 39.0 37.0 40.0 33.0 40.0 17 37.15566234851651 39.0 36.0 40.0 33.0 40.0 18 37.111122719041646 39.0 36.0 40.0 33.0 40.0 19 36.94668477503831 39.0 36.0 40.0 32.0 40.0 20 36.83657194595347 39.0 36.0 40.0 32.0 40.0 21 36.7166910433208 39.0 36.0 40.0 32.0 40.0 22 36.67363142498955 38.0 36.0 40.0 31.0 40.0 23 36.54058712912662 38.0 36.0 40.0 31.0 40.0 24 36.288758880066865 38.0 35.0 40.0 31.0 40.0 25 36.04063936481404 38.0 35.0 40.0 30.0 40.0 26 35.593519292380556 38.0 35.0 40.0 29.0 40.0 27 35.3303558991503 38.0 35.0 40.0 29.0 40.0 28 34.91422900125366 38.0 34.0 39.0 27.0 40.0 29 34.89901100431815 38.0 34.0 39.0 27.0 40.0 30 34.29692505920045 38.0 33.0 39.0 26.0 40.0 31 33.634036773923945 37.0 33.0 39.0 23.0 40.0 32 33.49312230115615 37.0 33.0 39.0 23.0 40.0 33 33.19003343083995 36.0 32.0 39.0 22.0 40.0 34 33.22088731021034 36.0 32.0 39.0 22.0 40.0 35 32.93144936620699 36.0 32.0 39.0 20.0 40.0 36 32.59848168268561 36.0 32.0 39.0 13.0 40.0 37 32.21014417049729 36.0 31.0 39.0 10.0 40.0 38 32.18313832010029 36.0 31.0 39.0 9.0 40.0 39 31.981142916840785 36.0 31.0 39.0 4.0 40.0 40 31.518369550076613 36.0 30.0 39.0 4.0 40.0 41 30.876776013372336 36.0 30.0 39.0 4.0 40.0 42 30.68491433347263 35.0 29.0 39.0 4.0 40.0 43 30.4261561498816 35.0 29.0 38.0 4.0 40.0 44 30.29406950828806 35.0 29.0 39.0 4.0 40.0 45 29.834430282769187 35.0 28.0 38.0 4.0 40.0 46 29.298944839114082 35.0 27.0 38.0 4.0 40.0 47 28.91058991502995 34.0 26.0 38.0 4.0 40.0 48 28.676817801922272 34.0 26.0 38.0 4.0 40.0 49 28.27275734782003 34.0 24.0 38.0 4.0 39.0 50 27.954450480568326 34.0 23.0 38.0 4.0 40.0 51 26.924867669591865 33.0 17.0 37.0 4.0 39.0 52 26.717195988299206 33.0 11.0 37.0 4.0 39.0 53 26.375034823791616 33.0 4.0 37.0 4.0 39.0 54 25.929551469564007 33.0 4.0 37.0 4.0 39.0 55 25.531027998328458 33.0 4.0 37.0 4.0 39.0 56 15.48373728931606 19.0 4.0 23.0 4.0 26.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.0661811006795503 1 2 -0.23454365481405404 1 3 -0.051275986430567855 1 4 -1.0167796102793076 1 5 -0.49795071340513886 1 6 -0.4465997456149964 1 7 -0.28792160584279713 1 8 -0.10765695447846468 1 9 -0.47589373814911085 1 10 0.1682372212536194 1 11 0.16563295460453276 1 12 -0.3394114041138465 1 13 0.499683044718509 1 14 0.5259618446770133 1 15 -0.0634594591146822 1 16 0.36350446027436334 1 17 0.7847964375285699 1 18 0.2550571647010784 1 19 0.638055439483729 1 20 0.2998701766478149 1 21 0.9872553820736698 1 22 0.09762899100416433 1 23 0.745634539148817 1 24 0.47456867587797547 1 25 0.8838625317500259 1 26 0.7766062523722255 1 27 -0.5267253623460775 1 28 1.753419564692365 1 29 1.2793259101826706 1 30 1.1867922742023822 1 31 1.595563300384768 1 32 1.952055223571648 1 33 1.5778621014277974 1 34 1.821504023039175 1 35 1.6933355677401067 1 36 2.2704754170143175 1 37 2.538326311077711 1 38 2.462323415798771 1 39 1.82343881118733 1 40 2.885657986635021 1 41 2.9836998734109983 1 42 1.73946193144425 1 43 2.585132036997294 1 44 3.052287118638997 1 45 2.8760491416856304 1 46 2.459402596907129 1 47 2.870727569422993 1 48 3.1423995177098654 1 49 3.430114535764215 1 50 2.6738523739723306 1 51 3.996896556943856 1 52 3.0873561396488114 1 53 3.5059656847732192 1 54 4.157087791853851 1 55 4.017096898947159 1 56 2.7224307879529377 2 1 -0.20506998956843603 2 2 -0.18361772888813022 2 3 -0.5813685790231631 2 4 -0.6880759065755981 2 5 -0.5465618245162531 2 6 -0.27067381968907256 2 7 -0.4453290132502019 2 8 -0.3090458433673504 2 9 -0.3161715159268894 2 10 0.14045944347584083 2 11 -0.7001077861362077 2 12 -0.4713558485582965 2 13 -0.2711502886148267 2 14 0.1949433261584943 2 15 0.40181831866309636 2 16 0.23387483064473713 2 17 -0.3726109698788349 2 18 0.41477938692329985 2 19 -0.04481493088663768 2 20 0.27209239887003633 2 21 -1.1933001734818873 2 22 -0.7889450830699118 2 23 -1.0900136089993282 2 24 -1.7268202130109174 2 25 -0.8429893201018288 2 26 0.5844766227425922 2 27 0.5427190820983654 2 28 1.6330491943219911 2 29 0.1913629472197087 2 30 -0.023855873945770156 2 31 0.8640818189032871 2 32 0.6210367050531289 2 33 0.5454546940203926 2 34 -0.6946996806645274 2 35 0.216483715888252 2 36 0.0644568984957985 2 37 0.980455940707337 2 38 1.381304897280252 2 39 0.23779066303917773 2 40 -0.07730497632794453 2 41 1.0253665400776661 2 42 -0.38553806855574635 2 43 0.9925394444046987 2 44 -0.06113880728693033 2 45 0.5820676602041459 2 46 0.5033840783886099 2 47 1.05128312497855 2 48 0.48730692511727014 2 49 0.6847441653938446 2 50 0.0905190406389984 2 51 1.267729890277188 2 52 0.4484672507599221 2 53 -0.31347875967122363 2 54 0.9232914955575531 2 55 1.3874672693175292 2 56 1.0025233805455294 3 1 -0.8413383333839519 3 2 -0.7447637806002234 3 3 -1.1208514581286835 3 4 -1.1056160882667285 3 5 -1.859979015291934 3 6 -1.1431406261181465 3 7 -0.23603481338996346 3 8 -0.693741860138843 3 9 -0.8717270714824465 3 10 -0.6666684391237396 3 11 -0.7109393724394906 3 12 -1.096093794050958 3 13 -0.874923873520487 3 14 -0.7362236899141763 3 15 -1.1684122893033617 3 16 -0.5673131497885251 3 17 -1.3016815498928125 3 18 -1.408071766116528 3 19 -0.8411112271829353 3 20 -0.4815763642326871 3 21 -1.3358578254734965 3 22 -0.30292132346124845 3 23 -0.2531862155681637 3 24 -0.8882457895308349 3 25 -0.7647223739103524 3 26 -0.2796044394516741 3 27 -1.5369454881322469 3 28 -1.339347730905125 3 29 -0.1852967313267655 3 30 -0.3222486063007679 3 31 -0.25821028452089223 3 32 -0.16862087705727902 3 33 -0.08172909354075131 3 34 -0.08219094551428441 3 35 0.4645619828344465 3 36 -0.772370494935366 3 37 -1.545400104016629 3 38 -1.1756482823144374 3 39 -1.2870171636554382 3 40 -0.7306942146228437 3 41 -0.377149183192774 3 42 -1.4201292635243021 3 43 -0.34254091897755146 3 44 0.6383720242993753 3 45 -0.8829917388175161 3 46 -0.02841186850167432 3 47 0.5774885757123016 3 48 0.20568568123187703 3 49 1.3224101332484892 3 50 1.0339152670540912 3 51 1.7016921544281303 3 52 1.8625133723532166 3 53 1.948575747666304 3 54 0.4648708107217736 3 55 -0.03321756646164431 3 56 -2.369943425883541 4 1 0.1242950897966395 4 2 0.25355158328117966 4 3 0.10050972785514745 4 4 0.44155372305402807 4 5 0.29669214373771524 4 6 0.577209778194522 4 7 0.44422125130005696 4 8 0.5798430455215353 4 9 0.7889872142318382 4 10 0.34680864982505 4 11 0.39182343079501436 4 12 0.5861838339813872 4 13 -0.1461502886148267 4 14 -0.11391910770393565 4 15 -0.1438166019718281 4 16 0.23255207932198374 4 17 0.08241548514762087 4 18 0.9008904980344141 4 19 0.6767459156742106 4 20 0.5558225576001945 4 21 0.6836839535022392 4 22 -0.11963291375774077 4 23 0.7724202534345324 4 24 0.9001639139732092 4 25 0.5445768174643106 4 26 0.22005863332460507 4 27 0.3720841614634409 4 28 -0.4191994829266861 4 29 -0.2206740898173294 4 30 0.0052446551547618014 4 31 0.2562775860990527 4 32 0.8478885569049837 4 33 -0.16023313666743633 4 34 0.9137659278010801 4 35 0.9433355677401067 4 36 1.327023036061938 4 37 1.1008263110777108 4 38 1.0605377015130557 4 39 1.9097483349968485 4 40 2.08803893901597 4 41 0.5015570162681406 4 42 0.3227952647775858 4 43 0.32322727509253113 4 44 0.9719299757818547 4 45 0.28378723692372176 4 46 1.2599978350023697 4 47 1.5195370932325183 4 48 1.1453757081860552 4 49 0.7455907262404047 4 50 1.072661897781856 4 51 0.9284441759914728 4 52 0.6290228063154792 4 53 0.8154894942970294 4 54 1.737444934710993 4 55 1.9873349941852538 4 56 0.2789784070005563 5 1 -0.2905400750385212 5 2 -0.15441544968584964 5 3 0.030454782800198643 5 4 -0.07767704617673843 5 5 0.21839544044101444 5 6 -0.3760869251021788 5 7 -0.19176775968895043 5 8 -0.574003108324618 5 9 -0.37172707148244655 5 10 0.1105449135613128 5 11 -0.22218755821597824 5 12 0.3128321856297376 5 13 0.09423432676978649 5 14 -0.08782020660503775 5 15 -0.299036382191602 5 16 -0.9185468217769142 5 17 -0.6703317675996345 5 18 -1.801032578888666 5 19 -0.3795727656444754 5 20 -0.4565400797624406 5 21 0.11225538207366981 5 22 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3.047009620705044 6 48 2.490155927966274 6 49 2.753832484482164 6 50 1.2635959637159218 6 51 0.9215760441233414 6 52 1.7155612678539391 6 53 1.528401582209117 6 54 1.2580493303153908 6 55 1.81837895022921 6 56 1.6855718135939632 7 1 -0.3482323827308278 7 2 -0.17364621891662324 7 3 0.049685552030972246 7 4 -0.34690781540751203 7 5 0.21839544044101444 7 6 -0.7222407712560255 7 7 0.30823224031104957 7 8 0.4836891993676886 7 9 0.45519600544063366 7 10 -0.8317627787463806 7 11 0.25858167255325526 7 12 0.08206295486051118 7 13 -0.05961182707636681 7 14 0.16217979339496225 7 15 -0.010574843730069006 7 16 -0.5723929756230675 7 17 -0.09340869067655433 7 18 0.16050588264980092 7 19 0.3704272343555246 7 20 -0.4373093105316741 7 21 -0.31082154100325 7 22 -1.7597428038676313 7 23 -1.0572500762357961 7 24 -0.8828031189938201 7 25 -1.8372913144038208 7 26 -1.1384578501918767 7 27 -2.4658279264486467 7 28 -0.29145223017943067 7 29 -2.6725971667404096 7 30 -1.5744256745155667 7 31 -0.6928982380767721 7 32 -1.0271114430950163 7 33 0.11311851168421327 7 34 -1.2922780282428725 7 35 -0.6816644322598933 7 36 -1.0259989419600402 7 37 -1.3991736889222892 7 38 -0.46556119958584574 7 39 -1.370471445222929 7 40 -2.7938291928521615 7 41 -1.064377049665925 7 42 0.5054875724698924 7 43 -0.11358591172065857 7 44 -0.44290518905330956 7 45 -1.096707268570782 7 46 0.7847231097276435 7 47 0.3931634668588906 7 48 0.9324636202739676 7 49 1.388447869097547 7 50 1.0135959637159218 7 51 1.017729890277188 7 52 1.1770997293924026 7 53 0.9514785052860404 7 54 0.9695877918538507 7 55 1.1453020271522867 7 56 1.8971102751324231 8 1 -1.2175416448972385 8 2 -0.7694076003922845 8 3 -0.3765310884713813 8 4 -1.6196707667418906 8 5 -0.34361397871126087 8 6 -0.6829943034381287 8 7 -0.5787379323734072 8 8 -0.8793406279478546 8 9 -0.9125433980130566 8 10 -0.0817627787463806 8 11 -0.37093167124580617 8 12 0.1907756707160857 8 13 0.5859925685280274 8 14 0.5876115045409591 8 15 0.23628543884450437 8 16 -0.44601934924944686 8 17 0.6844563014741496 8 18 -0.11696664482273178 8 19 -0.2696826557543659 8 20 -0.27325907505287006 8 21 -0.02549971996714362 8 22 -0.7114696484516188 8 23 0.1907876003733051 8 24 -0.3549381268431162 8 25 -1.0115456315152827 8 26 -0.006982183001923659 8 27 0.5404515084022208 8 28 0.12926990482841916 8 29 -0.7844496000214107 8 30 -0.45393901831462813 8 31 0.8149510554868087 8 32 0.5341130467009023 8 33 -0.254620891769477 8 34 0.7811128665765921 8 35 -0.7888072894027474 8 36 1.4979414034088805 8 37 1.6467446784246462 8 38 1.532476477023259 8 39 1.8281156819356283 8 40 2.1849777145261733 8 41 3.1342100774926323 8 42 3.345754448451057 8 43 1.7926150301945682 8 44 3.415807526802265 8 45 2.7404198899849455 8 46 2.606936610512573 8 47 1.6521901544570063 8 48 2.474457340839116 8 49 2.0313050119546894 8 50 3.151743530434917 8 51 1.7575258086445338 8 52 1.8713697450909912 8 53 2.5527343922562125 8 54 2.525710240833444 8 55 2.602130912552603 8 56 3.131019223327087 9 1 0.07270778820934254 9 2 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30 -1.486818836908732 120 31 -2.0248070699856022 120 32 -2.2122966282802032 120 33 -2.547137898572199 120 34 -1.2132181991830464 120 35 -2.4316644322598933 120 36 -2.1577653237264194 120 37 -0.4269514667000678 120 38 -0.7668432508678968 120 39 -0.46591304066452466 120 40 -1.799527198550166 120 41 -0.6413001265890017 120 42 -1.0892417722594523 120 43 0.49253944440469866 120 44 -0.8203980665461899 120 45 -1.1401545620180755 120 46 -0.07068999568546275 120 47 -0.3005687268733013 120 48 -1.2349152971049513 120 49 -1.630070649420972 120 50 -1.5391105889906314 120 51 -1.3248627023154036 120 52 -1.551532749240078 120 53 -1.7949602411527046 120 54 -0.7804122081461493 120 55 -1.3903105084602458 120 56 -2.053032175010026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 22.0 5 28.0 6 21.0 7 13.0 8 23.0 9 46.0 10 40.0 11 73.0 12 89.0 13 121.0 14 208.0 15 244.0 16 308.0 17 377.0 18 499.0 19 554.0 20 615.0 21 729.0 22 692.0 23 814.0 24 1066.0 25 1019.0 26 970.0 27 1141.0 28 1328.0 29 1467.0 30 1613.0 31 2051.0 32 2214.0 33 2814.0 34 3657.0 35 4827.0 36 6286.0 37 7694.0 38 8158.0 39 5611.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.73415517481543 1.1282908483075638 0.10621256442401449 0.03134141245298788 8 98.707497212932 0.8814102564102564 0.26477146042363436 0.1463210702341137 9 94.82692575567629 2.993104889260343 1.1822677253099318 0.9977016297534477 10 52.51776013372336 18.590681153363978 18.146677810279982 10.744880902632678 11 33.056484189998606 32.1510656080234 16.292310906811533 18.500139295166456 12 28.22816548265775 13.29920601755119 23.028973394623208 35.44365510516785 13 22.952361053071456 14.606839392673074 24.752751079537543 37.68804847471793 14 23.048126480011145 15.273715002089428 40.299832845800246 21.378325672099177 15 35.506337930073826 16.788549937317175 25.188048474717927 22.51706365789107 16 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24.541652143604043 29 38.68768417932309 17.464995030427733 20.274111144047847 23.573209646201327 30 26.500767834706128 17.328633254223092 33.875471171296944 22.295127739773836 31 28.119271660760113 27.532689722595627 21.844939857893543 22.50309875875072 32 41.6365509174392 17.416505176417186 19.59880584507411 21.348138061069502 33 28.953618329142216 18.075929030455434 20.794914780664993 32.17553785973736 34 25.301773080618396 30.05502663988121 23.432614202113722 21.21058607738667 35 25.68597294557657 21.764130168827993 34.08542766262365 18.46446922297179 36 28.807217540301426 32.6397873902857 19.722348498094206 18.83064657131867 37 27.355389242498422 20.018185633349653 32.522557179827935 20.103867944323984 38 38.92552875111523 19.85725031926246 20.014694820075924 21.202526109546387 39 29.112484029613032 18.019847034321018 20.00280028702942 32.864868649036524 40 27.49439461883408 19.673136210762333 31.050308295964125 21.782160874439462 41 25.299178245405006 18.623517249837924 32.598601790689116 23.478702714067946 42 35.03732781886369 16.92369703129929 23.51477340436718 24.52420174546984 43 22.982782200091172 15.301399165410107 36.088999544131575 25.62681909036715 44 22.987921392633627 16.93634626509826 24.341286397980472 35.73444594428764 45 23.168785620341957 17.20999561595791 34.61639631740464 25.00482244629548 46 22.371906190250662 19.01278745461243 23.048992264379308 35.5663140907576 47 21.280665426596006 29.00536973993613 24.89032393921314 24.82364089425473 48 24.07823583116792 29.447292646955546 23.885104291031674 22.58936723084486 49 25.340518149266305 29.969107631819142 21.777364319314753 22.913009899599803 50 34.31221020092735 18.719263734719686 22.9099339609386 24.058592103414362 51 24.293815066448975 30.403258369761765 23.552957286564492 21.749969277224768 52 25.35033189337267 19.53078354932743 32.992659712710285 22.12622484458961 53 25.417061777824607 18.243599199241388 34.19028553366347 22.149053489270536 54 25.377622130248685 30.437935547773183 21.344936149138633 22.839506172839506 55 24.280920000702828 29.000404125595207 21.49069632597122 25.227979547730744 56 34.26543947165039 18.227007658258977 20.696620529754796 26.810932340335842 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.0 9 6.0 10 9.5 11 13.0 12 18.5 13 26.0 14 28.0 15 22.0 16 16.0 17 24.5 18 33.0 19 78.5 20 124.0 21 112.0 22 130.5 23 161.0 24 179.5 25 198.0 26 231.5 27 265.0 28 336.0 29 449.0 30 491.0 31 492.0 32 493.0 33 607.5 34 722.0 35 592.0 36 462.0 37 552.0 38 621.0 39 600.0 40 598.5 41 597.0 42 599.0 43 601.0 44 668.5 45 736.0 46 1112.0 47 4493.5 48 7499.0 49 4670.0 50 1841.0 51 1642.0 52 1443.0 53 1769.5 54 2146.0 55 2196.0 56 2295.0 57 2394.0 58 2867.0 59 3340.0 60 3723.0 61 4106.0 62 4391.0 63 4603.0 64 4530.0 65 4114.0 66 3698.0 67 3316.0 68 2934.0 69 2632.0 70 2330.0 71 2046.0 72 1585.0 73 1408.0 74 1238.0 75 1068.0 76 927.5 77 787.0 78 629.0 79 377.0 80 283.0 81 230.0 82 177.0 83 131.5 84 86.0 85 67.0 86 48.0 87 36.5 88 22.5 89 20.0 90 11.5 91 3.0 92 2.0 93 1.0 94 2.0 95 3.0 96 1.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.041788549937317176 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0034823791614430975 25 0.024376654130101687 26 0.05571806658308956 27 0.06442401448669731 28 0.09054185819752054 29 0.14451873519988856 30 0.22287226633235824 31 0.2629196266889539 32 0.266402005850397 33 0.29251984956122024 34 0.3256024515949296 35 0.37261457027441147 36 0.41440312021172865 37 0.42485025769605794 38 0.4683799972140966 39 0.5136509263128569 40 0.5989692157682128 41 0.6250870594790361 42 0.6442401448669731 43 0.6929934531271765 44 0.6773227469006825 45 0.7086641593536704 46 0.7365231926452152 47 0.7765705530018109 48 0.8288062404234573 49 0.8009472071319126 50 0.8636300320378882 51 0.8201002925198495 52 0.8462181362306728 53 0.8462181362306728 54 1.5566234851650647 55 0.9036773923944839 56 0.8705947903607745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 57432.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.432720434600924 #Duplication Level Percentage of deduplicated Percentage of total 1 70.28745529209168 27.716255745925615 2 11.652757539629974 9.189998607048336 3 5.7844306089106725 6.842875052235688 4 3.408840022961099 5.3767934252681435 5 2.300525455910275 4.535798857779635 6 1.5454585596326225 3.656498119515253 7 0.9361063275489027 2.5839253377907787 8 0.7815604715856403 2.4655244463017136 9 0.5431182938137501 1.9274968658587546 >10 2.534552037797501 17.925546733528346 >50 0.16779264361725615 4.49575149742304 >100 0.05298715061597563 3.5433207967683518 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.004415595884664635 9.740214514556344 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 5594 9.740214514556344 No Hit AAGCGGGGGCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC 308 0.5362863908622371 No Hit AAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 278 0.4840507034405906 No Hit AAGCGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 212 0.3691321911129684 No Hit AAGCGGGGGCTGAGATTAAATGTCTGCACTTACCAGGCCCAGTTCAAAATGGTATT 202 0.3517202953057529 No Hit AAGCGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGC 189 0.32908483075637274 No Hit AAGCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 136 0.23680178297813068 No Hit AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 124 0.21590750800947206 No Hit AAGCGGGGATTCTGACTATGATTAAGGATAGCAGGAAGTCTATAAGCTATAACATA 122 0.21242512884802897 No Hit AAGCGGGGGTAATTGAAGTCAATAACCTGTTCCCAAGTGACTGTCATTTAGCGATA 120 0.20894274968658588 No Hit AAGCGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 120 0.20894274968658588 No Hit AAGCGGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT 114 0.19849561220225656 No Hit AAGCGGGGGTGGCATTGAGTACTGGGTCCTGAGTCGTGGGGCTTTCTTCTCACAGG 110 0.19153085387937038 No Hit AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA 98 0.1706365789107118 No Hit AAGCGGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 95 0.16541301016854715 No Hit AAGCGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT 94 0.1636718205878256 No Hit AAGCGGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA 91 0.15844825184566097 No Hit AAGCGGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 90 0.1567070622649394 No Hit AAGCGGGGGTGGATCTGAAAAATGATCAGGCATCCAGTGAGCTGCTTATCATACCC 87 0.15148349352277476 No Hit AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCGTATGCCGTCTTCTGCTTGAAAA 87 0.15148349352277476 Illumina Single End Adapter 1 (95% over 21bp) AAGCGGGGGGTGTGTGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG 86 0.1497423039420532 No Hit AAGCGGGGGGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGTGAG 81 0.14103635603844547 No Hit AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 80 0.1392951664577239 No Hit AAGCGGGGGAGCACTGTCAAGAACAGTTTCTTTCCATCAGTATTGCTAAAAGTTAT 78 0.13581278729628082 No Hit AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGT 76 0.13233040813483773 No Hit AAGCGGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 73 0.1271068393926731 No Hit AAGCGGGGGGTGTGGTTTAGTAGAGATATTAAAATTATTAAAAATACTATGTTGAT 69 0.12014208106978688 No Hit AAGCGGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 68 0.11840089148906534 No Hit AAGCGGGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 66 0.11491851232762224 No Hit AAGCGGGGGGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCCTGG 66 0.11491851232762224 No Hit AAGCGGGGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGC 63 0.10969494358545757 No Hit AAGCGGGGGAGACTCTGAACTTACCGAGTTTGCCCGCCGTGATTAGCTCGCGGAAG 63 0.10969494358545757 No Hit AAGCGGGGGGGAGCCGCTGCTGGCTCTGTGTCTCTGTCCTCAGCGTTCTCGTCCTC 63 0.10969494358545757 No Hit AAGCGGGGGCATGGTAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCG 63 0.10969494358545757 No Hit AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA 62 0.10795375400473603 No Hit AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 62 0.10795375400473603 No Hit AAGCGGGGGGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGA 61 0.10621256442401449 No Hit AAGCGGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT 59 0.1027301852625714 No Hit AAGCGGGGGACTCGAAGTGGAGCGCAGTCGGGGCCGGGCGTGTAGTCGGATCAGCC 59 0.1027301852625714 No Hit AAGCGGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG 58 0.10098899568184985 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.001741189580721549 0.0 0.0 0.0 0.0 10 0.001741189580721549 0.0 0.0 0.0 0.0 11 0.001741189580721549 0.0 0.0 0.0 0.0 12 0.003482379161443098 0.0 0.0 0.0 0.0 13 0.003482379161443098 0.0 0.0 0.0 0.0 14 0.005223568742164647 0.0 0.0 0.0 0.0 15 0.006964758322886196 0.0 0.0 0.0 0.0 16 0.006964758322886196 0.0 0.0 0.0 0.0 17 0.008705947903607745 0.0 0.0 0.0 0.0 18 0.010447137484329294 0.0 0.0 0.0 0.0 19 0.013929516645772392 0.0 0.0 0.0 0.0 20 0.01741189580721549 0.0 0.0 0.0 0.0 21 0.01741189580721549 0.0 0.0 0.0 0.0 22 0.019153085387937037 0.0 0.0 0.0 0.0 23 0.020894274968658588 0.0 0.0 0.0 0.0 24 0.020894274968658588 0.0 0.0 0.0 0.0 25 0.027859033291544783 0.0 0.0 0.0 0.0 26 0.027859033291544783 0.0 0.0 0.0 0.0 27 0.02960022287226633 0.0 0.0 0.0 0.0 28 0.038306170775874074 0.0 0.0 0.0 0.0 29 0.038306170775874074 0.0 0.0 0.0 0.0 30 0.041788549937317176 0.0 0.0 0.0 0.0 31 0.04527092909876027 0.0 0.0 0.0 0.0 32 0.047012118679481824 0.0 0.0 0.0 0.0 33 0.04875330826020337 0.0 0.0 0.0 0.0 34 0.05049449784092492 0.0 0.0 0.0 0.0 35 0.05049449784092492 0.0 0.0 0.0 0.0 36 0.05223568742164647 0.0 0.0 0.0 0.0 37 0.055718066583089566 0.0 0.0 0.0 0.0 38 0.05745925616381112 0.0 0.0 0.0 0.0 39 0.05745925616381112 0.0 0.0 0.0 0.0 40 0.06094163532525421 0.0 0.0 0.0 0.0 41 0.06094163532525421 0.0 0.0 0.0 0.0 42 0.06094163532525421 0.0 0.0 0.0 0.0 43 0.06442401448669731 0.0 0.0 0.0 0.0 44 0.06790639364814041 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACAT 20 3.8594365E-4 50.740448 49 TAACATA 20 3.8594365E-4 50.740448 50 TCATACC 20 3.8594365E-4 50.740448 49 ATCATAC 25 1.8902801E-5 50.740444 48 TCACAGG 25 1.8902801E-5 50.740444 50 TGTTGAT 15 0.007916057 50.74044 50 TCGCATC 15 0.007916057 50.74044 50 ATGTTGA 15 0.007916057 50.74044 49 CTATAAC 20 3.9970138E-4 50.38217 47 CTATGTT 15 0.008141211 50.382168 47 TACTCGC 15 0.008141211 50.382168 47 ATAAGCT 20 4.0144814E-4 50.33774 42 TAAGCTA 20 4.0144814E-4 50.33774 43 TTATCAT 25 1.9817211E-5 50.33774 46 AAATACT 15 0.008169687 50.33774 42 GTTCAAA 15 0.008169687 50.33774 42 CTGGACA 15 0.008169687 50.33774 43 CTTATCA 25 1.9817211E-5 50.33774 45 TGGACAA 15 0.008169687 50.33774 44 TTTGGGT 15 0.008169687 50.33774 44 >>END_MODULE