FastQCFastQC Report
Fri 17 Jun 2016
SRR1526662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3527
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2346.6345335979586055No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA982.778565352991211No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT421.1908137227105189No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.907286645874681No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.5954068613552594No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.5387014459880919No Hit
TTTAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.5103487383045081No Hit
TTTAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.4819960306209243No Hit
TTTAGGGAAGTTAGTGCATATGCGCACAAGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAG170.4819960306209243No Hit
TTTAGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.4252906152537568No Hit
TTTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.396937907570173No Hit
TTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.36858519988658917No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.36858519988658917No Hit
TTTAGGGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.28352707683583783No Hit
TTTAGGGGGAAGCTGTATCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.28352707683583783No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.25517436915225405No Hit
TTTAGGGGGATGGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.25517436915225405No Hit
TTTAGGGGGAAGCATGTATCAACGCAGAGTCGACTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT80.22682166146867025No Hit
TTTAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.22682166146867025No Hit
TTTAGGGGGAGTGTATAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.22682166146867025No Hit
TTTAGGGGGAAGCATTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.22682166146867025No Hit
TTTAGGGGGAAGTGTCAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.22682166146867025No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.22682166146867025No Hit
TTTAGGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.22682166146867025No Hit
TTTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.22682166146867025No Hit
TTTAGGGGGTGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.22682166146867025No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1984689537850865No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1984689537850865No Hit
TTTAGGGGGTAAGAAATAGTTTCCCTTGTTTAATGTTATGGTCATTGTTTTTCCTGTAGAGATT70.1984689537850865No Hit
TTTAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.1984689537850865No Hit
TTTAGGGGGAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTTCTGCTTAAACAGGGTT60.1701162461015027No Hit
TTTAGGGGGTGTCCGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1701162461015027No Hit
TTTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGCTCTTCCAGAGGTCCTGA60.1701162461015027No Hit
TTTAGGGGGATGTATCAACGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1701162461015027No Hit
TTTAGGGGGTCAACGCAGAGTCGACTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1701162461015027No Hit
TTTAGGGGGAGTACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1701162461015027No Hit
TTTAGGGGGCAGTGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.1701162461015027No Hit
TTTAGGGGAGCAGTGTACGCAGAGTCGACTTTTTTCTTTTTTCTTTTTTTTTTTTTTTTTTTTT50.14176353841791892No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.14176353841791892No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.14176353841791892No Hit
TTTAGGGGGACCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14176353841791892No Hit
TTTAGGGGGGTGACAAGCACTGCTATGCTAAGCCTGGACCGCTCGAGTGTTCTAGATGCACAAA50.14176353841791892No Hit
TTTAGGGGACTACAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14176353841791892No Hit
TTTAGGGGGGCTCTTATCTTTATATTCCAGTAAGTTATTTTGCTCTCACTGTTTTAACAACAAC50.14176353841791892No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11341083073433512No Hit
TTTAGGGGGAAAATTTATTGCAGCTCTCCAACACAGAATTATTTTTTCTTTTCAACATCTTGCT40.11341083073433512No Hit
TTTAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11341083073433512No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTTAGGGGGAAGCAGGGGTATCAA40.11341083073433512No Hit
TTTAGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11341083073433512No Hit
TTTAGGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11341083073433512No Hit
TTTAGGGGGGTGTGGCCATCAAGATACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11341083073433512No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11341083073433512No Hit
TTTAGGGGGACACATTGCTTCCTGATCCTTTCGGAAAATACATTTCAGATTTGAATCTTCAGAA40.11341083073433512No Hit
TTTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11341083073433512No Hit
TTTAGGGAGCAAAAGCAACAAAGGTTATGGCCATGGCTGGCCCTATCTAATAGCAAATCAACTT40.11341083073433512No Hit
TTTAGGGGGAACAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11341083073433512No Hit
TTTAGGGGGGTTGAATATCCGGAATGCGTTTGGATGGTGATTGCTTAACCATTGCATCCCTTCC40.11341083073433512No Hit
TTTAGGGGGGTTTGGTCGTTGATTAATATGTTGTTTGTTACATGGATTTTAATATTGTAAGCAC40.11341083073433512No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAGATGGATCACGCAGAGTCGACTTTTTTTTTTTT40.11341083073433512No Hit
TTTAGGGGGAGTCGAAGGTTGTGTGGCTTTTCTTTGTATGCTTTTTCTAGTCAGCCCAAAATAG40.11341083073433512No Hit
TTTAGGGGGAGGGGAAAAGCTTCATAACATTAGTCTTAGCAGTGATTTCGTGGATATGCTACCA40.11341083073433512No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGCTTACCCAGAGTCGACTTTTTTTTTTTTTTTTTT40.11341083073433512No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11341083073433512No Hit
TTTAGGGGGAACGCAGAGTCGACTTTTCTTTTTTTTTTTTTCAAAAAAAAAAAAAAAAAAAAAA40.11341083073433512No Hit
TTTAGGGGGAGGCCTGATGGATTTTGCATTCCGGAACCAAAGAGAGCACACGGCTCTTCTTAAA40.11341083073433512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAG201.5314264E-459.5000046
GGGGAGT201.5314264E-459.5000047
GGGGAAG201.5314264E-459.5000046
AGGGGGT302.3144275E-759.54
AGGGGAA150.003882861259.54
GGGGTGC150.003882861259.57
GGGAAGC150.003882861259.57
GGGAGTG150.004119365558.6376848
GGAAGCA150.004119365558.6376848
AGGGGGG1750.057.8000034
AGTGCAG150.004366436557.811
GGAGTGC150.004366436557.89
TAGGGGG3350.055.9477583
TTAGGGG3600.055.3680532
TTTAGGG3750.053.9466671
AGGGGGA1050.053.8333324
GGGGGGC459.567884E-1052.8888855
GGGGGAT402.5462214E-852.0625045
GGGGGGT1050.051.05
GGGGGGA356.7195833E-751.05
Produced by FastQC (version 0.11.5)