FastQCFastQC Report
Fri 17 Jun 2016
SRR1526661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4290
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3167.365967365967366No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1463.4032634032634035No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT831.934731934731935No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA531.2354312354312356No Hit
TTTAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA431.0023310023310024No Hit
TTTAGGGAAGTTAGTGCATATGCGCACAAGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAG380.8857808857808859No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.745920745920746No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.44289044289044294No Hit
TTTAGGGGGCGTTACTTCCGGCCTCCCTCTAGCTGCCATCTTGCGTCCCCGCGTGTGTGCGCCT180.4195804195804196No Hit
TTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.4195804195804196No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.34965034965034963No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.34965034965034963No Hit
TTTAGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.32634032634032634No Hit
TTTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.32634032634032634No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.30303030303030304No Hit
TTTAGGGGGAAGCTGTATCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.30303030303030304No Hit
TTTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.27972027972027974No Hit
TTTAGGGGGGCTCTTATCTTTATATTCCAGTAAGTTATTTTGCTCTCACTGTTTTAACAACAAC120.27972027972027974No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAA110.2564102564102564No Hit
TTTAGGGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGG110.2564102564102564No Hit
TTTAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.2564102564102564No Hit
TTTAGGGGGAGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.2564102564102564No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC110.2564102564102564No Hit
TTTAGGGGGGGCGTGATGTCAGGATGGTAGGTGAATGGTCTGGGAAGGCCGACCGGAGTGGGTG110.2564102564102564No Hit
TTTAGGGGGATCAACCTCAAACGATTCCCCGTTGAGAAATCGTTTTGGCAGACTGTCGTCGAAC100.2331002331002331No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.2331002331002331No Hit
TTTAGGGGAGCAGTGCACGCAGAGTCGACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.2331002331002331No Hit
TTTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACC100.2331002331002331No Hit
TTTAGGGGGTGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.2331002331002331No Hit
TTTAGGGGGAAGCATGTATCAACGCAGAGTCGACTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT90.2097902097902098No Hit
TTTAGGGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.2097902097902098No Hit
TTTAGGGGGTCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.2097902097902098No Hit
TTTAGGGGGTAAGAAATAGTTTCCCTTGTTTAATGTTATGGTCATTGTTTTTCCTGTAGAGATT90.2097902097902098No Hit
TTTAGGGGGACCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.2097902097902098No Hit
TTTAGGGGGTGTCCGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.2097902097902098No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.1864801864801865No Hit
TTTAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.1864801864801865No Hit
TTTAGGGGGCTCTGTACTGACCCTCCGAGCTCACACGCTACCTGCCCCGCAGCTCTTCAGCCCG70.16317016317016317No Hit
TTTAGGGGGAAGCCAAGGTTTGAGAACGTGGCTTATGTGGTCAGTGAGATGCCACCAAATCAAG70.16317016317016317No Hit
TTTAGGGGGGTGGTATAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.16317016317016317No Hit
TTTAGGGGGAGTCGAAGGTTGTGTGGCTTTTCTTTGTATGCTTTTTCTAGTCAGCCCAAAATAG70.16317016317016317No Hit
TTTAGGGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGCGCATGCCA70.16317016317016317No Hit
TTTAGGGGGAGTACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.16317016317016317No Hit
TTTAGGGGGCAGTGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.16317016317016317No Hit
TTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTGG60.13986013986013987No Hit
TTTAGGGAGCAAAAGCAACAAAGGTTATGGCCATGGCTGGCCCTATCTAATAGCAAATCAACTT60.13986013986013987No Hit
TTTAGGGGGAAAAAAAAAGGTAAAAACATGACAAATTACAGTAACATCTTCTTGTCCTTAATCT60.13986013986013987No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAA60.13986013986013987No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAA60.13986013986013987No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13986013986013987No Hit
TTTAGGGGGTCAACGCAGAGTCGACTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.13986013986013987No Hit
TTTAGGGGGCCTATATCTGCAATCTGTAAATGGTAAAATGTCTGTGTATTTTTTTAAATGTACC60.13986013986013987No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.13986013986013987No Hit
TTTAGGGGGTGGCTGCGACTGTTTAATAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTA50.11655011655011654No Hit
TTTAGGGGGAGGAAGAAGATGTGATTGAAGTTTATCAGGAACAAACGGGGGGTCACTCGACGGT50.11655011655011654No Hit
TTTAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGGAGTTAGAAAAAGGACAGAAGGAGTCGAAGGGGTTTGCAACCCCAAAGAAAGAACA50.11655011655011654No Hit
TTTAGGGGGACAGTTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11655011655011654No Hit
TTTAGGGGGGTGTGGCCATAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGGGTCCTCATGCATAGATTCATGATATGTTATGAAAAAAAAAAAAAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGGGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGA50.11655011655011654No Hit
TTTAGGGGAAATCGTAACCAAAGAACGACACAGAGAGATCAACAAGGTTGGTGGTACCACTCTG50.11655011655011654No Hit
TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGGGGCTATAAAAGGATTTGAAACCACAAACTTCCCAAGTCCTTTCATTGCCCGATCA50.11655011655011654No Hit
TTTAGGGGGAGTGCACTGCTTTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGCATTATCCCCCAAATTCACACATTTTCTTGTGGAAAATCCCCCATTTAACGTGAAA50.11655011655011654No Hit
TTTAGGGGGGTGTGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGGGTGCAGTGCTTTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGCAGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGGCGGGTGTTCTGCTGCCGAAGATTCCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGGGTGTGGCCATCAGTACTTCACCTGCTGCTTTGCCGGACTTCGGAGGACAGAGGGT50.11655011655011654No Hit
TTTAGGGGATAGCATTCATATAGGAATGAGCAGAGACATCTGAGAAGAGTGGGAGCTCATGGGT50.11655011655011654No Hit
TTTAGGGGGCTGTGGCCATCAAGATTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGGGCTCGGGTTGCCTTGCTGGTGTTGGGGGGATAGTGTGTGTTCGCTCGTTGATAAC50.11655011655011654No Hit
TTTAGGGGGAGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11655011655011654No Hit
TTTAGGGGGGAACCTGGGGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAAAGGTCC50.11655011655011654No Hit
TTTAGGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG50.11655011655011654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGATC700.057.38823720
TCAAGAT700.057.38823719
GGGGGGT1100.057.3882375
AAGATCC700.057.38823721
GGGGACA201.8865726E-457.3882376
AGATCCC650.057.38823722
TCCCCAA405.18412E-1057.38823725
ATCCCCA452.0008883E-1157.38823324
ATCCCAA150.004560568457.38823324
AGGGGGT150.004560568457.3882334
TCCCAAA150.004560568457.38823325
GGGGGAT150.004560568457.3882335
GATCCCC452.0008883E-1157.38823323
GATCCCA150.004560568457.38823323
GGGACAG150.004560568457.3882337
CCCCAAA452.0008883E-1157.38823326
CATCAAG750.057.3882317
CCATCAA750.057.3882316
GCCATCA750.057.3882315
ATCAAGA750.057.3882318
Produced by FastQC (version 0.11.5)