##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526661_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4290 Sequences flagged as poor quality 0 Sequence length 64 %GC 35 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.562703962703964 39.0 38.0 40.0 36.0 40.0 2 38.46060606060606 39.0 38.0 40.0 36.0 40.0 3 38.48065268065268 39.0 38.0 40.0 36.0 40.0 4 37.8981351981352 39.0 38.0 40.0 35.0 40.0 5 38.158041958041956 39.0 38.0 40.0 35.0 40.0 6 38.2044289044289 39.0 38.0 40.0 35.0 40.0 7 38.46969696969697 39.0 38.0 40.0 37.0 40.0 8 38.297902097902096 39.0 38.0 40.0 36.0 40.0 9 38.15874125874126 39.0 38.0 40.0 35.0 40.0 10 37.3018648018648 39.0 37.0 40.0 33.0 40.0 11 36.78065268065268 39.0 35.0 40.0 32.0 40.0 12 37.17785547785548 39.0 36.0 40.0 32.0 40.0 13 36.904428904428904 39.0 36.0 40.0 32.0 40.0 14 37.29300699300699 39.0 37.0 40.0 33.0 40.0 15 37.21072261072261 39.0 37.0 40.0 33.0 40.0 16 37.168764568764566 39.0 37.0 40.0 33.0 40.0 17 37.31398601398601 39.0 37.0 40.0 33.0 40.0 18 37.08904428904429 39.0 37.0 40.0 33.0 40.0 19 36.98904428904429 39.0 36.0 40.0 32.0 40.0 20 37.07692307692308 39.0 37.0 40.0 33.0 40.0 21 37.17226107226107 39.0 37.0 40.0 33.0 40.0 22 37.26550116550116 39.0 37.0 40.0 33.0 40.0 23 36.86433566433566 39.0 36.0 40.0 32.0 40.0 24 36.596969696969694 39.0 36.0 40.0 31.0 40.0 25 36.83006993006993 39.0 36.0 40.0 32.0 40.0 26 37.26480186480187 39.0 38.0 40.0 33.0 40.0 27 37.25011655011655 39.0 38.0 40.0 33.0 40.0 28 37.02750582750583 39.0 37.0 40.0 33.0 40.0 29 37.06899766899767 39.0 37.0 40.0 33.0 40.0 30 36.741025641025644 39.0 36.0 40.0 32.0 40.0 31 36.61724941724942 39.0 36.0 40.0 32.0 40.0 32 36.41981351981352 39.0 36.0 40.0 31.0 40.0 33 36.54778554778555 39.0 36.0 40.0 32.0 40.0 34 36.365967365967364 39.0 36.0 40.0 31.0 40.0 35 36.07086247086247 39.0 35.0 40.0 30.0 40.0 36 35.70745920745921 39.0 35.0 40.0 30.0 40.0 37 35.54102564102564 39.0 35.0 40.0 28.0 40.0 38 35.48717948717949 39.0 35.0 40.0 29.0 40.0 39 35.28741258741259 39.0 35.0 40.0 27.0 40.0 40 35.27039627039627 39.0 35.0 40.0 28.0 40.0 41 35.02307692307692 39.0 35.0 40.0 26.0 40.0 42 35.05827505827506 39.0 35.0 40.0 27.0 40.0 43 34.87435897435898 39.0 35.0 40.0 26.0 40.0 44 34.775757575757574 39.0 35.0 40.0 25.0 40.0 45 34.4932400932401 39.0 35.0 40.0 23.0 40.0 46 34.318414918414916 39.0 35.0 40.0 19.0 40.0 47 34.0995337995338 39.0 35.0 40.0 18.0 40.0 48 34.102797202797206 39.0 35.0 40.0 18.0 40.0 49 33.87972027972028 39.0 34.0 40.0 16.0 40.0 50 33.64195804195804 39.0 35.0 40.0 4.0 40.0 51 32.835198135198134 38.0 34.0 40.0 4.0 40.0 52 32.67878787878788 39.0 33.0 40.0 4.0 40.0 53 32.202097902097904 38.0 32.0 39.0 4.0 40.0 54 31.73939393939394 38.0 32.0 39.0 4.0 40.0 55 31.101631701631703 37.0 31.0 39.0 4.0 40.0 56 29.83752913752914 37.0 29.0 39.0 4.0 40.0 57 29.386946386946388 36.0 27.0 39.0 4.0 40.0 58 29.03310023310023 35.0 24.0 39.0 4.0 40.0 59 28.316317016317015 35.0 19.0 39.0 4.0 40.0 60 28.02960372960373 35.0 4.0 39.0 4.0 40.0 61 27.66829836829837 35.0 4.0 39.0 4.0 40.0 62 27.473193473193472 35.0 4.0 39.0 4.0 40.0 63 27.06130536130536 35.0 4.0 39.0 4.0 40.0 64 27.034265734265734 35.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.9617647058823522 1 2 -0.20546218487395862 1 3 0.07521008403361407 1 4 -0.09929971988795216 1 5 -1.212745098039214 1 6 0.38277310924370056 1 7 -1.0652661064425857 1 8 -0.9694677871148514 1 9 0.4959383753501285 1 10 -0.20672268907564728 1 11 1.5431372549019429 1 12 -0.46134453781512974 1 13 0.7519607843137308 1 14 0.4197478991596597 1 15 -1.5656862745098081 1 16 0.10714285714286831 1 17 0.6282913165266137 1 18 0.6015406162465027 1 19 1.357142857142847 1 20 0.29663865546218915 1 21 -0.7344537815126202 1 22 -0.9116246498599381 1 23 -1.7988795518207255 1 24 -2.4380952380952365 1 25 -0.7683473389355768 1 26 0.36834733893558536 1 27 0.5359943977591115 1 28 0.11106442577030151 1 29 -0.11750700280111204 1 30 -0.7584033613445271 1 31 -0.7163865546218489 1 32 1.3039215686274446 1 33 0.06512605042016872 1 34 -0.27170868347338484 1 35 -0.43977591036414765 1 36 -6.5844537815126 1 37 -7.665126050420149 1 38 -8.752380952380953 1 39 -6.098039215686271 1 40 -6.670168067226875 1 41 -7.00266106442578 1 42 -5.252661064425759 1 43 -5.971428571428568 1 44 -7.1016806722689125 1 45 -5.582913165266106 1 46 -6.090896358543411 1 47 -5.411764705882355 1 48 -5.515126050420172 1 49 -5.063865546218487 1 50 -4.619327731092433 1 51 -3.4312324929972036 1 52 -2.4676470588235233 1 53 -2.4935574229691824 1 54 -2.323249299719887 1 55 -3.3911764705882383 1 56 -1.147478991596639 1 57 -1.3903361344537757 1 58 -1.9383753501400633 1 59 0.4623249299719916 1 60 0.08921568627451038 1 61 -0.5280112044818033 1 62 1.164845938375347 1 63 -0.17450980392157334 1 64 0.6261904761904766 2 1 0.6882352941176464 2 2 0.4945378151260442 2 3 0.12521008403361122 2 4 0.10070028011205068 2 5 0.787254901960786 2 6 0.4327731092436977 2 7 0.6347338935574172 2 8 0.5305322128851486 2 9 0.9459383753501314 2 10 -0.8567226890756459 2 11 0.19313725490194145 2 12 -0.06134453781513116 2 13 1.3519607843137322 2 14 0.21974789915965687 2 15 -0.765686274509811 2 16 0.8071428571428712 2 17 0.9782913165266152 2 18 -0.3984593837534973 2 19 -0.7428571428571544 2 20 -1.0533613445378123 2 21 1.315546218487377 2 22 0.5383753501400648 2 23 0.551120448179276 2 24 0.6619047619047649 2 25 0.13165266106442175 2 26 0.9683473389355868 2 27 1.0859943977591087 2 28 0.21106442577030293 2 29 0.7824929971988865 2 30 0.14159663865547145 2 31 1.483613445378154 2 32 0.903921568627446 2 33 -4.684873949579831 2 34 -5.721708683473384 2 35 -4.439775910364148 2 36 -3.984453781512599 2 37 -4.01512605042015 2 38 -4.7023809523809526 2 39 -3.748039215686269 2 40 -3.420168067226875 2 41 -4.452661064425779 2 42 -3.85266106442576 2 43 -4.221428571428568 2 44 -6.1016806722689125 2 45 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2.9511204481792745 3 24 2.395238095238099 3 25 2.731652661064423 3 26 2.3683473389355854 3 27 2.2859943977591115 3 28 1.6110644257703015 3 29 2.382492997198888 3 30 1.7415966386554729 3 31 3.283613445378151 3 32 3.3039215686274446 3 33 2.6484593837535044 3 34 3.478291316526615 3 35 3.5602240896358524 3 36 4.082212885154064 3 37 4.2515406162465155 3 38 4.497619047619047 3 39 4.651960784313729 3 40 4.579831932773125 3 41 4.74733893557422 3 42 4.747338935574241 3 43 4.778571428571432 3 44 4.8983193277310875 3 45 5.167086834733894 3 46 5.659103641456589 3 47 5.838235294117645 3 48 5.734873949579828 3 49 5.936134453781513 3 50 6.130672268907567 3 51 7.318767507002796 3 52 7.282352941176477 3 53 7.756442577030818 3 54 7.8434173669467775 3 55 4.608823529411762 3 56 -2.480812324929971 3 57 -1.9736694677871078 3 58 1.8949579831932688 3 59 4.045658263305327 3 60 6.255882352941175 3 61 11.388655462184861 3 62 11.914845938375347 3 63 11.742156862745091 3 64 12.126190476190477 4 1 0.6882352941176464 4 2 0.69453781512604 4 3 0.7252100840336126 4 4 1.1007002801120507 4 5 0.3872549019607874 4 6 1.0327731092436991 4 7 0.8347338935574129 4 8 0.9305322128851472 4 9 1.145938375350127 4 10 1.5432773109243527 4 11 1.3931372549019443 4 12 1.5386554621848703 4 13 0.7519607843137308 4 14 1.4197478991596597 4 15 1.4343137254901919 4 16 1.6071428571428683 4 17 0.37829131652661374 4 18 1.4015406162464998 4 19 -0.7428571428571544 4 20 0.746638655462192 4 21 -0.4844537815126202 4 22 0.5383753501400648 4 23 0.3511204481792731 4 24 0.8619047619047606 4 25 -0.26834733893557683 4 26 1.5683473389355882 4 27 1.4859943977591143 4 28 1.8110644257703044 4 29 0.9824929971988894 4 30 1.9415966386554757 4 31 2.6836134453781497 4 32 2.5039215686274474 4 33 2.7151260504201673 4 34 1.678291316526618 4 35 2.960224089635851 4 36 3.6155462184874025 4 37 3.784873949579854 4 38 1.8976190476190453 4 39 3.4519607843137265 4 40 3.1798319327731264 4 41 3.74733893557422 4 42 3.5473389355742384 4 43 3.978571428571435 4 44 3.8983193277310875 4 45 3.3670868347338967 4 46 -2.54089635854341 4 47 -2.161764705882355 4 48 -2.66512605042017 4 49 -2.063865546218487 4 50 -1.6693277310924302 4 51 -1.281232492997205 4 52 -0.5176470588235205 4 53 0.1564425770308162 4 54 0.5767507002801118 4 55 0.20882352941176308 4 56 -6.547478991596638 4 57 -11.440336134453776 4 58 -11.438375350140063 4 59 -10.287675070028008 4 60 -10.810784313725488 4 61 -10.478011204481803 4 62 -10.085154061624653 4 63 -9.924509803921573 4 64 -9.673809523809524 5 1 -0.04509803921568789 5 2 -0.03879551820729432 5 3 -0.008123249299721635 5 4 0.23403361344538354 5 5 0.45392156862745026 5 6 -0.36722689075629944 5 7 0.10140056022407862 5 8 0.19719887955181292 5 9 0.4126050420167928 5 10 0.8766106442576884 5 11 1.7931372549019429 5 12 -0.7946778711484654 5 13 1.7519607843137308 5 14 0.08641456582632401 5 15 0.43431372549019187 5 16 0.2738095238095326 5 17 -0.28837535014005056 5 18 1.268207282913167 5 19 0.1904761904761827 5 20 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8.875490196078424 118 64 8.726190476190478 119 1 1.0382352941176478 119 2 1.0445378151260414 119 3 1.075210084033614 119 4 1.1507002801120478 119 5 1.537254901960786 119 6 1.3827731092437006 119 7 0.9347338935574143 119 8 1.2805322128851486 119 9 -0.004061624649871476 119 10 2.0432773109243527 119 11 2.293137254901943 119 12 2.0386554621848703 119 13 2.501960784313731 119 14 2.1697478991596597 119 15 1.6843137254901919 119 16 2.1071428571428683 119 17 0.37829131652661374 119 18 2.3515406162465027 119 19 1.107142857142847 119 20 1.7966386554621891 119 21 1.7655462184873798 119 22 1.838375350140062 119 23 2.2011204481792745 119 24 2.3119047619047635 119 25 1.7316526610644232 119 26 2.1183473389355854 119 27 2.0359943977591115 119 28 2.3610644257703015 119 29 1.382492997198888 119 30 1.9915966386554729 119 31 2.533613445378151 119 32 3.0539215686274446 119 33 3.0651260504201687 119 34 2.978291316526615 119 35 -1.1897759103641476 119 36 -5.5844537815126 119 37 -6.415126050420149 119 38 -5.502380952380953 119 39 -5.098039215686271 119 40 -4.920168067226875 119 41 -6.25266106442578 119 42 -7.002661064425759 119 43 -6.221428571428568 119 44 -8.101680672268913 119 45 -6.832913165266106 119 46 -5.340896358543411 119 47 -5.161764705882355 119 48 -5.265126050420172 119 49 -7.063865546218487 119 50 -6.119327731092433 119 51 -4.181232492997204 119 52 -4.217647058823523 119 53 -4.743557422969182 119 54 -3.323249299719887 119 55 -5.141176470588238 119 56 -3.897478991596639 119 57 -1.1403361344537757 119 58 -1.6883753501400633 119 59 -3.7876750700280084 119 60 -0.4107843137254896 119 61 0.22198879551819672 119 62 0.1648459383753469 119 63 1.3254901960784267 119 64 1.1261904761904766 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 11.0 5 3.0 6 1.0 7 5.0 8 8.0 9 2.0 10 2.0 11 3.0 12 4.0 13 3.0 14 7.0 15 7.0 16 22.0 17 19.0 18 14.0 19 33.0 20 29.0 21 27.0 22 39.0 23 47.0 24 47.0 25 44.0 26 40.0 27 57.0 28 62.0 29 82.0 30 103.0 31 138.0 32 156.0 33 192.0 34 246.0 35 265.0 36 357.0 37 470.0 38 773.0 39 963.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.6037296037296 0.30303030303030304 0.0 0.09324009324009325 8 96.38357442837145 2.96313579094727 0.4666355576294913 0.18665422305179655 9 88.39160839160839 8.554778554778556 1.4685314685314685 1.5850815850815851 10 44.19580419580419 36.62004662004662 9.79020979020979 9.393939393939393 11 20.675990675990676 33.939393939393945 34.84848484848485 10.536130536130536 12 49.44055944055944 21.84149184149184 19.02097902097902 9.696969696969697 13 20.41958041958042 23.216783216783217 37.17948717948718 19.184149184149184 14 41.655011655011656 31.07226107226107 14.871794871794872 12.400932400932401 15 42.98368298368299 24.848484848484848 14.801864801864802 17.365967365967368 16 16.177156177156178 30.44289044289044 20.0 33.37995337995338 17 25.780885780885782 27.156177156177158 16.456876456876458 30.606060606060602 18 23.146853146853147 43.28671328671329 16.736596736596738 16.82983682983683 19 18.927738927738925 27.575757575757574 37.71561771561772 15.78088578088578 20 15.61771561771562 31.11888111888112 18.484848484848484 34.77855477855478 21 15.244755244755245 42.61072261072261 26.526806526806528 15.61771561771562 22 19.393939393939394 43.12354312354312 19.976689976689975 17.505827505827508 23 31.7016317016317 35.10489510489511 19.3006993006993 13.892773892773894 24 17.34265734265734 49.044289044289044 20.93240093240093 12.680652680652681 25 19.02541384938214 27.605502448123104 30.892982047097224 22.476101655397528 26 24.88339552238806 25.55970149253731 16.534514925373134 33.02238805970149 27 22.224813432835823 25.23320895522388 16.06809701492537 36.473880597014926 28 21.06368089573128 31.957079542803825 17.63470958712386 29.344529974341032 29 22.748483434437706 33.24778348110126 19.178721418572096 24.82501166588894 30 20.070011668611436 47.58459743290548 21.353558926487747 10.991831971995332 31 17.848810079328047 46.75688287447504 25.804946336910874 9.589360709286048 32 13.278879813302217 46.72112018669778 29.918319719953324 10.081680280046674 33 14.008872285780994 48.307261265468135 26.7335979453654 10.950268503385477 34 15.681233933161954 49.56765599439122 25.77705071278336 8.974059359663473 35 13.644859813084112 50.95794392523364 26.77570093457944 8.621495327102805 36 13.367609254498714 47.04370179948586 25.96401028277635 13.624678663239074 37 12.058892264547792 46.85674222949287 29.235802757653655 11.848562748305678 38 13.884992987377279 46.423562412342214 28.938756428237493 10.75268817204301 39 10.310965630114566 46.85527238718728 32.779985971475334 10.053776011222821 40 12.976385316810848 45.3121346738368 31.026420388122517 10.685059621229835 41 11.31369798971482 45.23141654978962 32.234689107059374 11.220196353436185 42 12.836100070142622 45.52256254383914 30.558802899228432 11.082534486789806 43 10.965630114566286 47.41641337386018 30.67570727145195 10.94224924012158 44 12.672433949029694 47.205985503857846 31.61094224924012 8.51063829787234 45 12.771929824561404 47.1812865497076 30.45614035087719 9.5906432748538 46 12.210526315789473 46.59649122807018 30.666666666666664 10.526315789473683 47 11.812865497076023 46.94736842105263 29.5906432748538 11.649122807017545 48 13.286549707602338 47.368421052631575 28.654970760233915 10.690058479532164 49 11.368421052631579 48.888888888888886 28.46783625730994 11.27485380116959 50 10.996724379971923 48.52597098736546 28.965839962564345 11.511464670098269 51 12.491228070175438 50.03508771929825 29.426900584795323 8.046783625730994 52 12.189985961628452 49.391670566214316 27.86616752456715 10.55217594759008 53 11.815629386991109 49.532054281703324 28.895648104819845 9.756668226485727 54 12.701754385964911 47.85964912280702 29.28654970760234 10.152046783625732 55 12.657931679925127 49.62564342536266 27.86616752456715 9.850257370145062 56 14.763687412260179 48.52597098736546 26.34534394010295 10.36499766027141 57 12.944756554307116 50.09363295880149 26.33426966292135 10.627340823970037 58 14.633575275111216 49.63708733317724 25.942402247717162 9.78693514399438 59 13.669402110199297 50.99648300117233 24.712778429073857 10.621336459554513 60 15.303491914694165 47.504101242090464 26.013592688071245 11.17881415514413 61 15.6586966713549 46.952648851383024 24.91795593061416 12.470698546647913 62 15.667447306791567 47.02576112412178 24.59016393442623 12.716627634660421 63 14.426229508196723 47.96252927400468 24.33255269320843 13.278688524590162 64 15.900562851782363 46.20075046904315 24.765478424015008 13.133208255159476 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 9.5 5 41.0 6 63.0 7 111.5 8 160.0 9 92.0 10 15.5 11 7.0 12 14.5 13 23.5 14 25.0 15 27.5 16 31.0 17 32.0 18 47.0 19 62.0 20 58.5 21 77.0 22 99.0 23 157.5 24 270.0 25 324.0 26 406.0 27 339.5 28 191.0 29 206.0 30 181.0 31 141.0 32 151.5 33 162.0 34 148.5 35 113.5 36 92.0 37 96.0 38 96.5 39 93.0 40 87.0 41 87.0 42 93.0 43 87.0 44 81.0 45 102.0 46 128.0 47 133.0 48 114.0 49 97.0 50 99.0 51 93.5 52 91.5 53 95.0 54 103.0 55 96.0 56 81.0 57 94.5 58 108.0 59 99.0 60 72.5 61 55.0 62 46.5 63 51.0 64 64.0 65 51.0 66 29.0 67 20.0 68 18.5 69 17.0 70 10.5 71 3.5 72 3.0 73 2.0 74 1.0 75 1.0 76 2.5 77 2.5 78 1.0 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.09324009324009325 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.023310023310023312 26 0.046620046620046623 27 0.046620046620046623 28 0.06993006993006994 29 0.09324009324009325 30 0.11655011655011654 31 0.09324009324009325 32 0.11655011655011654 33 0.16317016317016317 34 0.2564102564102564 35 0.2331002331002331 36 0.2564102564102564 37 0.2564102564102564 38 0.27972027972027974 39 0.30303030303030304 40 0.30303030303030304 41 0.27972027972027974 42 0.30303030303030304 43 0.30303030303030304 44 0.30303030303030304 45 0.34965034965034963 46 0.34965034965034963 47 0.34965034965034963 48 0.34965034965034963 49 0.34965034965034963 50 0.372960372960373 51 0.34965034965034963 52 0.372960372960373 53 0.372960372960373 54 0.34965034965034963 55 0.372960372960373 56 0.372960372960373 57 0.4195804195804196 58 0.44289044289044294 59 0.5827505827505828 60 0.5361305361305362 61 0.5594405594405595 62 0.4662004662004662 63 0.4662004662004662 64 0.6060606060606061 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 4290.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.13286713286714 #Duplication Level Percentage of deduplicated Percentage of total 1 79.02896776825786 45.151515151515156 2 11.913504691962466 13.613053613053614 3 3.875968992248062 6.643356643356643 4 1.9991840065279476 4.568764568764569 5 1.0199918400652794 2.9137529137529135 6 0.36719706242350064 1.2587412587412588 7 0.28559771521827826 1.1421911421911422 8 0.08159934720522236 0.372960372960373 9 0.24479804161566704 1.2587412587412588 >10 1.0199918400652794 9.137529137529137 >50 0.08159934720522236 3.1701631701631703 >100 0.08159934720522236 10.76923076923077 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 316 7.365967365967366 No Hit TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 146 3.4032634032634035 No Hit TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83 1.934731934731935 No Hit TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 1.2354312354312356 No Hit TTTAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43 1.0023310023310024 No Hit TTTAGGGAAGTTAGTGCATATGCGCACAAGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAG 38 0.8857808857808859 No Hit TTTAGGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.745920745920746 No Hit TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.44289044289044294 No Hit TTTAGGGGGCGTTACTTCCGGCCTCCCTCTAGCTGCCATCTTGCGTCCCCGCGTGTGTGCGCCT 18 0.4195804195804196 No Hit TTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.4195804195804196 No Hit TTTAGGGGGGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.34965034965034963 No Hit TTTAGGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.34965034965034963 No Hit TTTAGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.32634032634032634 No Hit TTTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.32634032634032634 No Hit TTTAGGGGGGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.30303030303030304 No Hit TTTAGGGGGAAGCTGTATCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.30303030303030304 No Hit TTTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.27972027972027974 No Hit TTTAGGGGGGCTCTTATCTTTATATTCCAGTAAGTTATTTTGCTCTCACTGTTTTAACAACAAC 12 0.27972027972027974 No Hit TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAA 11 0.2564102564102564 No Hit TTTAGGGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGG 11 0.2564102564102564 No Hit TTTAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.2564102564102564 No Hit TTTAGGGGGAGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.2564102564102564 No Hit TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC 11 0.2564102564102564 No Hit TTTAGGGGGGGCGTGATGTCAGGATGGTAGGTGAATGGTCTGGGAAGGCCGACCGGAGTGGGTG 11 0.2564102564102564 No Hit TTTAGGGGGATCAACCTCAAACGATTCCCCGTTGAGAAATCGTTTTGGCAGACTGTCGTCGAAC 10 0.2331002331002331 No Hit TTTAGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.2331002331002331 No Hit TTTAGGGGAGCAGTGCACGCAGAGTCGACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.2331002331002331 No Hit TTTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACC 10 0.2331002331002331 No Hit TTTAGGGGGTGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.2331002331002331 No Hit TTTAGGGGGAAGCATGTATCAACGCAGAGTCGACTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.2097902097902098 No Hit TTTAGGGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.2097902097902098 No Hit TTTAGGGGGTCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.2097902097902098 No Hit TTTAGGGGGTAAGAAATAGTTTCCCTTGTTTAATGTTATGGTCATTGTTTTTCCTGTAGAGATT 9 0.2097902097902098 No Hit TTTAGGGGGACCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.2097902097902098 No Hit TTTAGGGGGTGTCCGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.2097902097902098 No Hit TTTAGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.1864801864801865 No Hit TTTAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8 0.1864801864801865 No Hit TTTAGGGGGCTCTGTACTGACCCTCCGAGCTCACACGCTACCTGCCCCGCAGCTCTTCAGCCCG 7 0.16317016317016317 No Hit TTTAGGGGGAAGCCAAGGTTTGAGAACGTGGCTTATGTGGTCAGTGAGATGCCACCAAATCAAG 7 0.16317016317016317 No Hit TTTAGGGGGGTGGTATAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.16317016317016317 No Hit TTTAGGGGGAGTCGAAGGTTGTGTGGCTTTTCTTTGTATGCTTTTTCTAGTCAGCCCAAAATAG 7 0.16317016317016317 No Hit TTTAGGGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGCGCATGCCA 7 0.16317016317016317 No Hit TTTAGGGGGAGTACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.16317016317016317 No Hit TTTAGGGGGCAGTGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.16317016317016317 No Hit TTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTGG 6 0.13986013986013987 No Hit TTTAGGGAGCAAAAGCAACAAAGGTTATGGCCATGGCTGGCCCTATCTAATAGCAAATCAACTT 6 0.13986013986013987 No Hit TTTAGGGGGAAAAAAAAAGGTAAAAACATGACAAATTACAGTAACATCTTCTTGTCCTTAATCT 6 0.13986013986013987 No Hit TTTAGGGGGGTGCAGTGCTTTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAA 6 0.13986013986013987 No Hit TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAA 6 0.13986013986013987 No Hit TTTAGGGGGAGTGCAGTGCTTTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.13986013986013987 No Hit TTTAGGGGGTCAACGCAGAGTCGACTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.13986013986013987 No Hit TTTAGGGGGCCTATATCTGCAATCTGTAAATGGTAAAATGTCTGTGTATTTTTTTAAATGTACC 6 0.13986013986013987 No Hit TTTAGGGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.13986013986013987 No Hit TTTAGGGGGTGGCTGCGACTGTTTAATAAAAACACAGCACTCTGCAAACACGAAAGTGGACGTA 5 0.11655011655011654 No Hit TTTAGGGGGAGGAAGAAGATGTGATTGAAGTTTATCAGGAACAAACGGGGGGTCACTCGACGGT 5 0.11655011655011654 No Hit TTTAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGGAGTTAGAAAAAGGACAGAAGGAGTCGAAGGGGTTTGCAACCCCAAAGAAAGAACA 5 0.11655011655011654 No Hit TTTAGGGGGACAGTTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.11655011655011654 No Hit TTTAGGGGGGTGTGGCCATAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGGGTCCTCATGCATAGATTCATGATATGTTATGAAAAAAAAAAAAAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGGGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGA 5 0.11655011655011654 No Hit TTTAGGGGAAATCGTAACCAAAGAACGACACAGAGAGATCAACAAGGTTGGTGGTACCACTCTG 5 0.11655011655011654 No Hit TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGGGGCTATAAAAGGATTTGAAACCACAAACTTCCCAAGTCCTTTCATTGCCCGATCA 5 0.11655011655011654 No Hit TTTAGGGGGAGTGCACTGCTTTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGCATTATCCCCCAAATTCACACATTTTCTTGTGGAAAATCCCCCATTTAACGTGAAA 5 0.11655011655011654 No Hit TTTAGGGGGGTGTGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGGGTGCAGTGCTTTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGCAGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGGCGGGTGTTCTGCTGCCGAAGATTCCGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGGGTGTGGCCATCAGTACTTCACCTGCTGCTTTGCCGGACTTCGGAGGACAGAGGGT 5 0.11655011655011654 No Hit TTTAGGGGATAGCATTCATATAGGAATGAGCAGAGACATCTGAGAAGAGTGGGAGCTCATGGGT 5 0.11655011655011654 No Hit TTTAGGGGGCTGTGGCCATCAAGATTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGGGCTCGGGTTGCCTTGCTGGTGTTGGGGGGATAGTGTGTGTTCGCTCGTTGATAAC 5 0.11655011655011654 No Hit TTTAGGGGGAGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5 0.11655011655011654 No Hit TTTAGGGGGGAACCTGGGGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAAAGGTCC 5 0.11655011655011654 No Hit TTTAGGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 5 0.11655011655011654 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.023310023310023312 0.0 0.0 0.0 0.0 21 0.023310023310023312 0.0 0.0 0.0 0.0 22 0.09324009324009325 0.0 0.0 0.0 0.0 23 0.09324009324009325 0.0 0.0 0.0 0.0 24 0.16317016317016317 0.0 0.0 0.0 0.0 25 0.2331002331002331 0.0 0.0 0.0 0.0 26 0.27972027972027974 0.0 0.0 0.0 0.0 27 0.27972027972027974 0.0 0.0 0.0 0.0 28 0.32634032634032634 0.0 0.0 0.0 0.0 29 0.372960372960373 0.0 0.0 0.0 0.0 30 0.3962703962703963 0.0 0.0 0.0 0.0 31 0.3962703962703963 0.0 0.0 0.0 0.0 32 0.4195804195804196 0.0 0.0 0.0 0.0 33 0.48951048951048953 0.0 0.0 0.0 0.0 34 0.48951048951048953 0.0 0.0 0.0 0.0 35 0.5594405594405595 0.0 0.0 0.0 0.0 36 0.5594405594405595 0.0 0.0 0.0 0.0 37 0.5594405594405595 0.0 0.0 0.0 0.0 38 0.5594405594405595 0.0 0.0 0.0 0.0 39 0.5827505827505828 0.0 0.0 0.0 0.0 40 0.6060606060606061 0.0 0.0 0.0 0.0 41 0.6293706293706294 0.0 0.0 0.0 0.0 42 0.6526806526806527 0.0 0.0 0.0 0.0 43 0.6993006993006993 0.0 0.0 0.0 0.0 44 0.745920745920746 0.0 0.0 0.0 0.0 45 0.7925407925407926 0.0 0.0 0.0 0.0 46 0.7925407925407926 0.0 0.0 0.0 0.0 47 0.7925407925407926 0.0 0.0 0.0 0.0 48 0.8391608391608392 0.0 0.0 0.0 0.0 49 0.8391608391608392 0.0 0.0 0.0 0.0 50 0.8624708624708625 0.0 0.0 0.0 0.0 51 0.8857808857808858 0.0 0.0 0.0 0.0 52 0.9557109557109557 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGATC 70 0.0 57.388237 20 TCAAGAT 70 0.0 57.388237 19 GGGGGGT 110 0.0 57.388237 5 AAGATCC 70 0.0 57.388237 21 GGGGACA 20 1.8865726E-4 57.388237 6 AGATCCC 65 0.0 57.388237 22 TCCCCAA 40 5.18412E-10 57.388237 25 ATCCCCA 45 2.0008883E-11 57.388233 24 ATCCCAA 15 0.0045605684 57.388233 24 AGGGGGT 15 0.0045605684 57.388233 4 TCCCAAA 15 0.0045605684 57.388233 25 GGGGGAT 15 0.0045605684 57.388233 5 GATCCCC 45 2.0008883E-11 57.388233 23 GATCCCA 15 0.0045605684 57.388233 23 GGGACAG 15 0.0045605684 57.388233 7 CCCCAAA 45 2.0008883E-11 57.388233 26 CATCAAG 75 0.0 57.38823 17 CCATCAA 75 0.0 57.38823 16 GCCATCA 75 0.0 57.38823 15 ATCAAGA 75 0.0 57.38823 18 >>END_MODULE