FastQCFastQC Report
Fri 17 Jun 2016
SRR1526660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4203
Sequences flagged as poor quality0
Sequence length64
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3067.28051391862955No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1273.021651201522722No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT781.8558172733761598No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA501.1896264572924102No Hit
TTTAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA481.1420413990007139No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.6423982869379015No Hit
TTTAGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.5948132286462051No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.5472281703545087No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.4996431120628123No Hit
TTTAGGGAAGTTAGTGCATATGCGCACAAGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAG200.4758505829169641No Hit
TTTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.3330954080418749No Hit
TTTAGGGGGGCTCTTATCTTTATATTCCAGTAAGTTATTTTGCTCTCACTGTTTTAACAACAAC140.3330954080418749No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC120.28551034975017847No Hit
TTTAGGGGGAAGCATGTATCAACGCAGAGTCGACTTCTTTTTTTTTTTTTTTTTTTTTTTTTTT110.26171782060433024No Hit
TTTAGGGGGATCAACCTCAAACGATTCCCCGTTGAGAAATCGTTTTGGCAGACTGTCGTCGAAC110.26171782060433024No Hit
TTTAGGGGGTAAGAAATAGTTTCCCTTGTTTAATGTTATGGTCATTGTTTTTCCTGTAGAGATT100.23792529145848204No Hit
TTTAGGGGGTGTCCGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.23792529145848204No Hit
TTTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.23792529145848204No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAA90.21413276231263384No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.21413276231263384No Hit
TTTAGGGGGCGTTACTTCCGGCCTCCCTCTAGCTGCCATCTTGCGTCCCCGCGTGTGTGCGCCT90.21413276231263384No Hit
TTTAGGGGGGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGA90.21413276231263384No Hit
TTTAGGGGGAGTACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.21413276231263384No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.19034023316678564No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.19034023316678564No Hit
TTTAGGGGGGTGTGGCCATCAAGATNCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.19034023316678564No Hit
TTTAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.19034023316678564No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.19034023316678564No Hit
TTTAGGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.19034023316678564No Hit
TTTAGGGGGTGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.19034023316678564No Hit
TTTAGGGGGAAGCTGTATCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.16654770402093744No Hit
TTTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16654770402093744No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16654770402093744No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16654770402093744No Hit
TTTAGGGGGACCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.16654770402093744No Hit
TTTAGGGGGGTGGTATAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.16654770402093744No Hit
TTTAGGGGGGTGACAAGCACTGCTATGCTAAGCCTGGACCGCTCGAGTGTTCTAGATGCACAAA70.16654770402093744No Hit
TTTAGGGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16654770402093744No Hit
TTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16654770402093744No Hit
TTTAGGGGAGCAGTGCACGCAGAGTCGACTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.16654770402093744No Hit
TTTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACC70.16654770402093744No Hit
TTTAGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16654770402093744No Hit
TTTAGGGGGCAGTTCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.16654770402093744No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.16654770402093744No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.14275517487508924No Hit
TTTAGGGGGACCGAACTATGTTAACCGGAGGTACAGCTTACATGATGTCACCATTTCTTTACGA60.14275517487508924No Hit
TTTAGGGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14275517487508924No Hit
TTTAGGGGGAAGCCAAGGTTTGAGAACGTGGCTTATGTGGTCAGTGAGATGCCACCAAATCAAG60.14275517487508924No Hit
TTTAGGGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGG60.14275517487508924No Hit
TTTAGGGGGAAGCATTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14275517487508924No Hit
TTTAGGGGGATGGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14275517487508924No Hit
TTTAGGGGGAGTCGAAGGTTGTGTGGCTTTTCTTTGTATGCTTTTTCTAGTCAGCCCAAAATAG60.14275517487508924No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.14275517487508924No Hit
TTTAGGGGGAGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.14275517487508924No Hit
TTTAGGGGGAAGATGGTATAACAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.14275517487508924No Hit
TTTAGGGGGGTGTGGCATGGTGGTGCATGCCTTTAGTTCCAGCTACTTGGGAGGCTGAGGCATG60.14275517487508924No Hit
TTTAGGGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGCGCATGCCA60.14275517487508924No Hit
TTTAGGGGGCATGCCATGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.14275517487508924No Hit
TTTAGGGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG60.14275517487508924No Hit
TTTAGGGGGTGGAAGTTGGGAGAGAATCAGGAAAAACAACTAATGGGTACTATGCTTAATACCT50.11896264572924102No Hit
TTTAGGGGGTGGTACCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11896264572924102No Hit
TTTAGGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG50.11896264572924102No Hit
TTTAGGGGGAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11896264572924102No Hit
TTTAGGGGGGCTCTTTAGACAACAGGATGTCCGGGAGACTTTGAGTAAGGACACGAGTGTGCGG50.11896264572924102No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11896264572924102No Hit
TTTAGGGAGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11896264572924102No Hit
TTTAGGGGGTCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11896264572924102No Hit
TTTAGGGGTGGACAAGACAGTCCCACAGTCCAGCCTAACTATGGGACAGCTTTACGAGAAGGAA50.11896264572924102No Hit
TTTAGGGCGTTCAAATTTTTGGAGCCAACTTTAAGACTGGAGTATTGCGAGGCTTTGGCAATGC50.11896264572924102No Hit
TTTAGGGGGCTGAATATAGCACATTTATACAGTCTGCGAAAGATCTTCAAAAGCACCCAGGAGA50.11896264572924102No Hit
TTTAGGGGGGCAGACCCGCGTGTGGCGCAGGCAAGGACCCTGAAAATAAACAGCCGCTGCTTTG50.11896264572924102No Hit
TTTAGGGGGGTGCAGTGCTTTTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11896264572924102No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG50.11896264572924102No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGG50.11896264572924102No Hit
TTTAGGGGGAGTGCAGTGCTTTTGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAA50.11896264572924102No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11896264572924102No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC50.11896264572924102No Hit
TTTAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11896264572924102No Hit
TTTAGGGGGGTTGGCACAATGGCTCACACCTGTAAGCCATCATACCTGTAAGTTTTTGGGAAGC50.11896264572924102No Hit
TTTAGGGGGCAGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.11896264572924102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG150.00418709958.6144639
GCCGTGC150.00418709958.6144640
GTATGCG150.004394288657.91666831
GGGCATG150.004394288657.9166687
GGCATGC150.004394288657.9166688
GGCGTGA150.004394288657.9166689
GGCCGTA150.004394288657.91666818
TATGCGT150.004394288657.91666832
GAACGGC150.004394288657.91666814
ATGCGTT150.004394288657.91666833
GCGTGAA150.004394288657.91666810
CCGTAAA150.004394288657.91666820
TTAGGGA150.004394288657.9166682
CGGCCGT150.004394288657.91666817
GGGGCGT150.004394288657.9166687
GGGCGTG150.004394288657.9166688
GCCGTAA150.004394288657.91666819
GTGAACG150.004394288657.91666812
TAGGGGT150.004394288657.9166683
CGTGAAC150.004394288657.91666811
Produced by FastQC (version 0.11.5)