FastQCFastQC Report
Fri 17 Jun 2016
SRR1526659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478
Sequences flagged as poor quality0
Sequence length56
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3098.884416331224843No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA992.8464634847613572No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT852.443933294997125No Hit
TTTAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.8913168487636572No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.7475560667050029No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.690051753881541No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA210.6037952846463485No Hit
TTTAGGGGGTGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.5175388154111558No Hit
TTTAGGGAAGTTAGTGCATATGCGCACAAGTGTGTGTGTGTGTGAGAGAGAGAGAG160.46003450258769407No Hit
TTTAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT150.43128234617596317No Hit
TTTAGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.43128234617596317No Hit
TTTAGGGGGCGTTACTTCCGGCCTCCCTCTAGCTGCCATCTTGCGTCCCCGCGTGT150.43128234617596317No Hit
TTTAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.43128234617596317No Hit
TTTAGGGGGAAGCTGTATCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT140.4025301897642324No Hit
TTTAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.3450258769407705No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA110.3162737205290397No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.3162737205290397No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA110.3162737205290397No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA100.28752156411730884No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAG100.28752156411730884No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA100.28752156411730884No Hit
TTTAGGGGGTGTCCGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.28752156411730884No Hit
TTTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG90.2587694077055779No Hit
TTTAGGGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGC90.2587694077055779No Hit
TTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.2587694077055779No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCGAAAAAAAAAAAAAAAAAAAAAAAAAA90.2587694077055779No Hit
TTTAGGGGGGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAA90.2587694077055779No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAA80.23001725129384704No Hit
TTTAGGGGGGCTCTTATCTTTATATTCCAGTAAGTTATTTTGCTCTCACTGTTTTA80.23001725129384704No Hit
TTTAGGGGGTAAGAAATAGTTTCCCTTGTTTAATGTTATGGTCATTGTTTTTCCTG70.2012650948821162No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCGCAAAAAAAAAAAAAAAAAAAAAAAAAA70.2012650948821162No Hit
TTTAGGGGGGTTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.2012650948821162No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCTCAAAAAAAAAAAAAAAAAAAAAAAAAA70.2012650948821162No Hit
TTTAGGGGGATCAACCTCAAACGATTCCCCGTTGAGAAATCGTTTTGGCAGACTGT70.2012650948821162No Hit
TTTAGGGAGCAAAAGCAACAAAGGTTATGGCCATGGCTGGCCCTATCTAATAGCAA70.2012650948821162No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAGG70.2012650948821162No Hit
TTTAGGGGGAAGCATGTATCAACGCAGAGTCGACTTCTTTTTTTTTTTTTTTTTTT70.2012650948821162No Hit
TTTAGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA70.2012650948821162No Hit
TTTAGGGGGAAGCCAAGGTTTGAGAACGTGGCTTATGTGGTCAGTGAGATGCCACC70.2012650948821162No Hit
TTTAGGGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCA60.17251293847038526No Hit
TTTAGGGAAGTACAGCCCTGACTGCACCATCATCGTGGTTTCCAACCCAGTGGATA60.17251293847038526No Hit
TTTAGGGGGGAGGAAGAAGGAGAGGAATACTAAATTAAATGTCACAAGGTGCTGCT60.17251293847038526No Hit
TTTAGGGGTGAACAAGTACCGTGAGGGAAAGGCGAAGAACCCCGGGAGGGGAGTGA60.17251293847038526No Hit
TTTAGGGAGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA60.17251293847038526No Hit
TTTAGGGGGGCTACTAGGGAGGCTGAGGTAGGAGAATCACTTGAACCCAGGAGGCA60.17251293847038526No Hit
TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.17251293847038526No Hit
TTTAGGGGGAGGTTGTAAATATTTGTGGTTTACCTTCTGACGACTTTTCTGCTTAA50.14376078205865442No Hit
TTTAGGGGGAGTACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14376078205865442No Hit
TTTAGGGGGACCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14376078205865442No Hit
TTTAGGGGGCAGTTCCGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14376078205865442No Hit
TTTAGGGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.14376078205865442No Hit
TTTAGGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.14376078205865442No Hit
TTTAGGGGGAGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA50.14376078205865442No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.14376078205865442No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.14376078205865442No Hit
TTTAGGGGGGTGTGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.14376078205865442No Hit
TTTAGGGGGGTGACAAGCACTGCTATGCTAAGCCTGGACCGCTCGAGTGTTCTAGA50.14376078205865442No Hit
TTTAGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14376078205865442No Hit
TTTAGGGGGGTTTGGTCGTTGATTAATATGTTGTTTGTTACATGGATTTTAATATT50.14376078205865442No Hit
TTTAGGGGGGTGTAGTGCTCAAAGTGGGAGGGCGTGAAACCGTTAGGACCCGAAAG50.14376078205865442No Hit
TTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGA50.14376078205865442No Hit
TTTAGGGGGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.14376078205865442No Hit
TTTAGGGGGAGATGGACAACGCAGAGTCGACCTTTTTTCTTTTTTTTTTTTTTTTT50.14376078205865442No Hit
TTTAGGGGGAGTCGAAGGTTGTGTGGCTTTTCTTTGTATGCTTTTTCTAGTCAGCC50.14376078205865442No Hit
TTTAGGGGGATGGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.14376078205865442No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAA50.14376078205865442No Hit
TTTAGGGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG50.14376078205865442No Hit
TTTAGGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCAATG50.14376078205865442No Hit
TTTAGGGGGATTCTATCTGGTGAAAAACCTGGACCACACAAAATTCAAGGTATCTC40.11500862564692352No Hit
TTTAGGGGGAAGCAGTGCTATCAACGCAGAGTGCAGGGGTGAGATGGACTGAGCCA40.11500862564692352No Hit
TTTAGGGGTGCTTACTTCGGGATAGAGCCTTCTCAGATCCCGAAGCTACCAGCCAC40.11500862564692352No Hit
TTTAGGGGGCAGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAA40.11500862564692352No Hit
TTTAGGGGGATGATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit
TTTAGGGGGTGATATCAACGCGAGTACATGCTTTTAGGGGGGTAAAAAAAAAAAAA40.11500862564692352No Hit
TTTAGGGGGAGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA40.11500862564692352No Hit
TTTAGGGGGTCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11500862564692352No Hit
TTTAGGGGGACAGTGGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit
TTTAGGGGGGTGGTATAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit
TTTAGGGGGAAGTCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit
TTTAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCT40.11500862564692352No Hit
TTTAGGGGGTTACGCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit
TTTAGGGGGCATCAACCAGAGCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit
TTTAGGGGGTGGCAGAGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit
TTTAGGGGGAGTACCTGAAACCATGTGCCTACAAACCGTTGGAGCCTCCTTGTGGG40.11500862564692352No Hit
TTTAGGGGGGTAAAGACCGCCCCTACTGACATGGACAGGCAGAAGCCTAATTCAAT40.11500862564692352No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGGTGTGGCCATCAAGATCCCAAAAAAA40.11500862564692352No Hit
TTTAGGGAGGTGTGGCCATCAAGATCCCCAATCCTGTTTTTATTGTTGAATCCATT40.11500862564692352No Hit
TTTAGGGGGGTGTGGCCATTAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11500862564692352No Hit
TTTAGGGGGAAGCATTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit
TTTAGGGGGGAATATTGATGTACATCCATCAGAAAAAGCTCTCATTGTTCAGTATG40.11500862564692352No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGGCGGGTGTTCTGCTGCCGAAGATTCCGG40.11500862564692352No Hit
TTTAGGGGGGTTCAGACCACCGAAAGCAGTTCCCAGCCACATCCAAAAAAAAAAAA40.11500862564692352No Hit
TTTAGGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11500862564692352No Hit
TTTAGGGGGACATGGTCACCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA40.11500862564692352No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAC40.11500862564692352No Hit
TTTAGGGGGTGTCGAAGGTGGAGTTCCCGCTCCAACCGGCTGGGAATAAGGAAATT40.11500862564692352No Hit
TTTAGGGGGCAGTGTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit
TTTAGGGGGGGCGTGATGTCAGGATGGTAGGTGAATGGTCTGGGAAGGCCGACCGG40.11500862564692352No Hit
TTTAGGGGGACCGAACTATGTTAACCGGAGGTACAGCTTACATGATGTCACCATTT40.11500862564692352No Hit
TTTAGGGGGCAGTGGTTCAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit
TTTAGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.11500862564692352No Hit
TTTAGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG40.11500862564692352No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC40.11500862564692352No Hit
TTTAGGGGGGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTT40.11500862564692352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGT307.7867844E-749.9565244
AGGGGGC307.7867844E-749.9565244
GGGGGAT150.007767155849.9565245
AGGGGGG1350.048.106284
TAGGGGG3000.047.4586943
GGGGGGT950.047.327235
TTAGGGG3500.047.1018642
TAGGGGA401.0167787E-743.7119563
TTTAGGG3950.043.632911
AGGGGGA1150.041.268434
GGCCATC750.039.96521414
TGTGGCC750.039.96521411
GTGGCCA750.039.96521412
GTGTGGC750.039.96521410
TGGCCAT750.039.96521413
GGGGGAA701.8189894E-1239.2515535
GGGGGTG900.038.8550726
AAGATCC655.4569682E-1138.42809321
GGTGTGG655.4569682E-1138.4280939
AGATCCC601.207809E-937.46739222
Produced by FastQC (version 0.11.5)