FastQCFastQC Report
Fri 17 Jun 2016
SRR1526658_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526658_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009
Sequences flagged as poor quality0
Sequence length64
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27613.73817819810851No Hit
CCTTTCGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA1437.117969138875062No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG793.9323046291687405No Hit
CCTTTCGGGCGTTAAGGGCGTATGGCGGATGCCTAGGCTTTCAGAGGCGAAGAAGGACGTGGTA211.0452961672473868No Hit
CCTTTCGGGGCAGGGATTTAGATGCCTGCTGATTTCTGTTGGATATCTGCCATGAATAATAGAA211.0452961672473868No Hit
CCTTTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.9955201592832255No Hit
CCTTTCGGGGATGTGCAGAAACTGCACAAAACACCTCAAGCAGATCCTCCTCCAGAAGAGGAGT170.8461921353907417No Hit
CCTTTCGGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGG150.7466401194624191No Hit
CCTTTCGGGGCCTATTGAAGAATGAACCGGCGAGTTACTGTCATTAGCGAGGTTAAGCGGAAAA150.7466401194624191No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTT150.7466401194624191No Hit
CCTTTCGGGGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGA130.6470881035340966No Hit
CCTTTCGGGGCTAGAGGGTTTATATTTGGCCCTCCTATTGCTTTGGCCATTGGGGCAAAAATTT120.5973120955699354No Hit
CCTTTCGGGATGTGTTTGTTGTTGCAAATCATGCAAACTTGTTATGTATTATGGGAAATAATTT120.5973120955699354No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.5973120955699354No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC120.5973120955699354No Hit
CCTTTCCCAGCTTGAATTCAATAAATCTGTCCACTCCCCTTTTGTGGGAGTGAATGTTTTAACA110.5475360876057741No Hit
CCTTTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.5475360876057741No Hit
CCTTTCGGGATGTAACTTAAGTTTTTAATTGTATGTTTTCAGAGAAAGTTATATTAATTGTCTC100.49776007964161273No Hit
CCTTTCAGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA100.49776007964161273No Hit
CCTTTCGGGAAAAATCGAAATTATGGATCTTATCAAGTATTGTAAGGATTCGTATTCGGAATTA100.49776007964161273No Hit
CCTTTCGGGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATA90.44798407167745147No Hit
CCTTTCCCTTGGCTCAAATAGAATACAATACGTATAATAAAGCATTAATAAATATAAAAAAAAA90.44798407167745147No Hit
CCTTTCGGGGTTGTTTGGCAATCACGCATGTTATGTTCAATTAGACTAAAAACAAAAAAAAAAA90.44798407167745147No Hit
CCTTTCGGGTGCCCACCTCAGTAATATCATCTATAGTCTCATAAAATGTTCCTAGTGTAAGGAC80.3982080637132902No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG80.3982080637132902No Hit
CCTTTCGGGCGCCGCCGCCAATGCTTAATAGCAAGTTTCGTCTATAGAAGTTTAGAGAAAATCG80.3982080637132902No Hit
CCTTTCGGGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.3982080637132902No Hit
CCTTTCGGGCTAAGAACATATATTGTTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.34843205574912894No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.34843205574912894No Hit
CCTTTCGGGGACATCATACAAGCCTTACCTTGGCCATAGCTCCATCGTATGCCGTCTTCTGCTT70.34843205574912894Illumina Single End Adapter 1 (95% over 21bp)
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG70.34843205574912894No Hit
CCTTTCGGGGTGTTCCTGAGATCTACGGTCATCCATGTACTGTGTGTACGTGGTGTTGTCTAAT70.34843205574912894No Hit
CCTTTCGGGCTGCCTTGTCATTTGTACTGTTATCATAATAAATAAAGAATTATTAGTATTAAAA70.34843205574912894No Hit
CCTTTCGGGAAAGAGCACAGATAAAGAATAATCAGCCCTGAGGATTAGAGTGCAAATGGAAGGC60.2986560477849677No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.2986560477849677No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGG60.2986560477849677No Hit
CCTTTCGGGGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGA60.2986560477849677No Hit
CCTTTCGGGATCTTAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCGTAGTAGCGGCGAGC60.2986560477849677No Hit
CCTTTCGGGGTAATCCTTTCCCACAAAGGACCGATATATCCTATAAATTATAAGAGAAATTAGG50.24888003982080636No Hit
CCTTTCGGGCAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTA50.24888003982080636No Hit
CCTTTCGGGGGGTAATAAGCAGTTCTACAGCCACATGATTAATCTTTTCTTAATGTGGGTTGTA50.24888003982080636No Hit
CCTTTCGGGGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACC50.24888003982080636No Hit
CCTTTCGGGGTGCGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.24888003982080636No Hit
CCTTTCGGGGCCGGAGTGATCTAGTCATGAGCAGGTTGAAAGAAGGGTAAAACCTTGTGGAGGA50.24888003982080636No Hit
CCTTTCGGGATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATACT50.24888003982080636No Hit
CCTTTCGGGACTTTTCTTGGAGTTGTGCTTGTTTATTCTTCTATAATTCCCCGATCCGAAAACC50.24888003982080636No Hit
CCTTTCGGGGTGGCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.24888003982080636No Hit
CCTTTCGGGGCTGTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.24888003982080636No Hit
CCTTTCGGGTGTCGTAAAGACAAATAACTTCTTCTTGGTTGCAGGCATGCCGACCTGTCACTCG50.24888003982080636No Hit
CCTTTCGGGACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAA50.24888003982080636No Hit
CCTTTCGGGATGAGAGGATGGAGGTCTATACTCCATATTCTTTCAAACAAATTATACGACCAGT50.24888003982080636No Hit
CCTTTCGGGTGCATGGTTAGCCATTACGATACCAACCTCTGCCTGTTAATCTGTGAACCACTTG50.24888003982080636No Hit
CCTTTCTGTTGCTGCTGTGAGCGCCTGTTTGCCTTCTCCGTTCACTCACCACTTTGCCTGTTTG50.24888003982080636No Hit
CCTTTCGGGGGATGCTAACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAA50.24888003982080636No Hit
CCTTTCGGGGGAATCGGGCGGCTACGTCGTCTGAATTGTAGTCTGGAGAAGCGTCCTCAGCGAC50.24888003982080636No Hit
CCTTTCGGGATTGTATGGCCGGGGTGTTATGCTCGTATATGGTTCTTGATATAGTTGCCTGTGC50.24888003982080636No Hit
CCTTTCGGGCCTTGGTCTCTCCCTCTGGCTTACTCCCTTCAGTCAAATTCTTTCTTCTTAGAAG50.24888003982080636No Hit
CCTTTCGGGATCGGTGTTTATGTAGTTTTCTGGAACCATTATGGAACCAAAAGTCAAGTAAATG50.24888003982080636No Hit
CCTTTCGGGTGACTGAACAACTAACACTTTCACAGTTCCACCTTATACACGCCCTCTGTTAACG50.24888003982080636No Hit
CCTTTCGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGAAGAAAGA50.24888003982080636No Hit
CCTTTCGGGCGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGG50.24888003982080636No Hit
CCTTTCGGGAGTTAAAGATGATTTAGTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.24888003982080636No Hit
CCTTTCGGGAGGCCCGAAAGTGATAAGATGTGATGCCTATGTTTTTGAAAAAAAAAAAAAAAAA40.1991040318566451No Hit
CCTTTCGGGAAGTATAATTTTATGTTTACTGAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1991040318566451No Hit
CCTTTCGGGAACTTCCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1991040318566451No Hit
CCTTTCGGGTCGTGAAACTGGTTTCGGGTTAGCATGTAATGAACTTGGTCACATAGTCTTTGTG40.1991040318566451No Hit
CCTTTCGGGAGGAATATTGATGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1991040318566451No Hit
CCTTTCGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGGACGCTTCTCCAGACTACAATT40.1991040318566451No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGG40.1991040318566451No Hit
CCTTTCGGGATCCACGTCTGAGTCTCGTCATGTATTCAGAGAATAATTAATATTATAAGCCTTC40.1991040318566451No Hit
CCTTTCGGGATTGCACCCACTATCGTAAAACATAGGGGTGCAATGGCCCCGATCAGACCCAGTG40.1991040318566451No Hit
CCTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1991040318566451No Hit
CCTTTCGGGATTCGACTCAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1991040318566451No Hit
CCTTTCGGGCGACCCCAGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGG40.1991040318566451No Hit
CCTTTCGGGAGTATAAATATATATATTTTCTATATATAAGATGTATAATATAAGGCTCCACAAT40.1991040318566451No Hit
CCTTTCGGGGCAACGCAAAGTGCAGTGCTCCTTTCGGGGTGTGGCCATCAAGATCCCCAAAAAA40.1991040318566451No Hit
CCTTTCGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGGGTTTAAGCATATAAATAAGCGGA40.1991040318566451No Hit
CCTTTCGGGGTGGGGTGGGGTGGGGAAATCTTCCTAGGCTTTTAGGAGATTTCACTCCTCTTTT40.1991040318566451No Hit
CCTTTCGGGGGTGTGCACCGGTCGTCCCATCCCTTCTGTCGGCGATGCGTGCCTGGCCTTAACT40.1991040318566451No Hit
CCTTTCGGGCTGTCAACTGAACATTCATTTGTGCACGCACGATGCTGTGTTTGTTGTTTCCTTG40.1991040318566451No Hit
CCTTTCGGGATATAAAGAGTGGATTTATGTTCGAGTAAATAACTTGGGGCTTGCAATGGCTAAT40.1991040318566451No Hit
CCTTTCGGGGACTTGAGTTCCAAGTTCTTAGGCGGGTGACATCTCGTAGCCAGATGATAAATTT40.1991040318566451No Hit
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCC40.1991040318566451No Hit
CCTTTCGGGGAACTAGAAAAAGCAATTTTAAAATTCATATGGAACCAAAAAAGAGGCTGCATGC30.14932802389248384No Hit
CCTTTCGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA30.14932802389248384No Hit
CCTTTCGGGGTTCATGGCTGGGATCCATGATGATATTGTATGGAATCCTAAATTTGCATCTATT30.14932802389248384No Hit
CCTTTCGGGAAGCCGGCGAACTGAAACATCTAAGTAGCCGGAGGAAAAGAAAGTAAAAACGATT30.14932802389248384No Hit
CCTTTCGGGCCGCCTTGTCATTTGTACTGTTATCATAATAAATAAAGAATTATTAGTATTAAAA30.14932802389248384No Hit
CCTTTCGGGGCGTGAAGAAGGGAAACGGCTACGGTATCGATATTGTTGAGACCGAGAAGAGCTA30.14932802389248384No Hit
CCTTTCGGGGAGGCAGGAGAATTGCTTGAACCTGGGAGGTGGAGGTTGTGGTGACCTGAGATCA30.14932802389248384No Hit
CCTTTCGGGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATT30.14932802389248384No Hit
CCTTTCGGGCGGGTGTTCTGCTGCCGGAGCTGTTGCTGCAGCCTAGGCTGGGCACTGGCCGGAT30.14932802389248384No Hit
CCTTTCGGGGGGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14932802389248384No Hit
CCTTTCGGGGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGC30.14932802389248384No Hit
CCTTTCGGGAACTCGAGCAGGGACGAAAGTCGGGCTTAGTGATCCGGTGGTTCCGCATGGAAGG30.14932802389248384No Hit
CCTTTCGGGGCTTGAAATGAATCAAATAATATGCAATTCCAAAGCTAGGGTTTTGTTGTTCAGA30.14932802389248384No Hit
CCTTTCGGGGGTAGGCCGCGTTTGGATACATTCGAACCAACATGGATGTTCCATCAGTACCATT30.14932802389248384No Hit
CCTTTCGGGAGAATAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14932802389248384No Hit
CCTTTCGGGGCGACCCCAGGTCAGGCGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA30.14932802389248384No Hit
CCTTTCGGGGTGTGGCCATCAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA30.14932802389248384Illumina Single End Adapter 2 (100% over 34bp)
CCTTTCGGGGTCACCCCTAACAAGGCAATCTGGAAGCAGAAATACAAACTCCAGCCCCACCACA30.14932802389248384No Hit
CCTTTCGGGATGTCATTGAATTTCACTTCGGTTACTTGTGCTGACCTGAATGGGTCTACTTTAT30.14932802389248384No Hit
CCTTTCGGGGATTTAGGTGTTATTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14932802389248384No Hit
CCTTTCGGGAGTTGATTGAGATCAACTAAGGAAATGGGGGTCTACTACTAATAACTTTTACCTG30.14932802389248384No Hit
CCTTTCGGGAAGTACATTCTGATTATTTAGTGTGCTGCTGGTCAGTCTAGAAATGTAATAATGG30.14932802389248384No Hit
CCTTTCGGGGGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14932802389248384No Hit
CCTTTCGGGGTGTGGCCATCAATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14932802389248384No Hit
CCTTTCGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGG30.14932802389248384No Hit
CCTTTCGGGATGTGGCTGGGAACTGCTTTCGGTGGTCTGAACCCAAAAAAAAAAAAAAAAAAAA30.14932802389248384No Hit
CCTTTCGGGGTGTGCCATCAAGATCCCCAAAAAAAGGACTTTCTTTATTGAGGGCAATGGGATG30.14932802389248384No Hit
CCTTTCGGGAAGCCATGCATGTGTAAGTATGAACTATTTCAGACTGTGAAACTGCGAATGGCTC30.14932802389248384No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCTCTTCTGGTGTCTGAAGACAGGAACATTGTACTTAT30.14932802389248384No Hit
CCTTTCAGGACTGGTGTTTGTGTCGGTCTGCAGCTGTGTATGGTATTATGTCTTATAATCCTGC30.14932802389248384No Hit
CCTTTCGGGCGAGGAAAGGCTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGC30.14932802389248384No Hit
CCTTTCGGGGTCTAGAGAGTGAGTCCTACTACTGCTATAAAATAAGATGATATGGTTGGCTGAT30.14932802389248384No Hit
CCTTTCGGGGTGTGGCCATCAAGATCCCCAAAACTCGTCTAATATATACTTTATTCTGAGCAAT30.14932802389248384No Hit
CCTTTCGGGAGTCAGATTAAAGCCAACTACTACGAAGACAAAACCGACGACGTCCTGCTCGAAA30.14932802389248384No Hit
CCTTTCGGGGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCA30.14932802389248384No Hit
CCTTTCGGGGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACGA30.14932802389248384No Hit
CCTTTCGGGCATGAACGCAGAGTCGACTTTTTTTTTTGACCATCTCATCCCTGCGTGTCTCCGA30.14932802389248384No Hit
CCTTTCGGGGAACTTTATCAAACGTCTTATTGCCTCTGGTAAATGATAATGACTCTTTTTGAGG30.14932802389248384No Hit
CCTTTCGGGACAAACAATTGTTGAAGAAGAAGGATACATTGCAGTAGTTGAAGAAGCAGCAGCT30.14932802389248384No Hit
CCTTTCGGGGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGATCCCTGGGAAT30.14932802389248384No Hit
CCTTTCGGGGCCCTGCCCTGAATGTATAGGGGTGTGGGTGGTAACGCGGGGAAGTGAAACATCT30.14932802389248384No Hit
CCTTTCGGGATGCCTGTAGTCCTAGCTACTTGGGAGGCTGAGGTGGGAGAACTCTTGAGCCCAG30.14932802389248384No Hit
CCTTTCGGGGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGTGTTG30.14932802389248384No Hit
CCTTTCGGGGTGTTCCTGAGATCTGCGGTCATCCATGTACTGTGTGTACGTGGTGTTGTCTAAT30.14932802389248384No Hit
CCTTTCGGGATGGGCTTTTGCCTGAATTATGGTTGTTTTAGCTGTAGATGTTTCAAACCTTGAT30.14932802389248384No Hit
CCTTTCGGGCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCA30.14932802389248384No Hit
CCTTTCGGGAGAGGGGAGAAATGTGGCGATCGAGTAAACATTTTTGTTCTAGAATTTATGAGTC30.14932802389248384No Hit
CCTTTCGGGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCC30.14932802389248384No Hit
CCTTTCGGGAACACAAATAAATGACAAATGCTTGAAGTGTTGGATATCTCAAGTTACCCTGACT30.14932802389248384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAG201.6409205E-457.4256
CGGGCGC150.004195927657.4256
CCTTTCG2000.057.4251
GGGGAGT201.6409205E-457.4257
TTCGGGC256.278615E-657.4254
TTCGGGA403.0559022E-1057.4254
TCGGGGG358.594725E-957.4255
TCGGGGA201.6409205E-457.4255
TCGGGAT201.6409205E-457.4255
TCGGGAC150.004195927657.4255
CTTTCGG1950.057.4252
TTTCGGG2000.055.9893763
TTCGGGG1300.055.2163474
TCGGGGT503.092282E-1151.68255
CGGGGTG458.731149E-1051.0444456
GGGGTGT402.4701876E-850.2468767
TCGGGGC301.8511337E-547.8541685
TGTGGCC456.25987E-844.66388711
GGGTGTG456.25987E-844.6638878
GTGTGGC456.25987E-844.66388710