FastQCFastQC Report
Fri 17 Jun 2016
SRR1526655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100162
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.3703999520776342No Hit
CAACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3490.34843553443421654No Hit
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3060.3055050817675366No Hit
CAACAAGGGGGAGGCACGGCCTCCGCCGTGTGGTCCAGCGGGGAAGGCTCCCTCTGTCTCTCTT2710.2705616900620994No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2540.25358918551945847No Hit
CAACAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.24360535931790497No Hit
CAACAAGGGGAGTGGGCTCTACTGGGCTCTCCTTGAGGGCCCCGGGTCTTCACACTCACGGTCC2300.22962800263573013No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2130.21265549809308917No Hit
CAACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2020.20167328927138037No Hit
CAACAAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2000.1996765240310697No Hit
CAACAAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG1900.18969269782951617No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1900.18969269782951617No Hit
CAACAAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGAC1740.17371857590703063No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1660.16573151494578783No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA1590.15874283660470037No Hit
CAACAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1590.15874283660470037No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1520.15175415826361296No Hit
CAACAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1500.14975739302330224No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1470.1467622451628362No Hit
CAACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1450.1447654799225255No Hit
CAACAAGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC1400.13977356682174877No Hit
CAACAAGGGGCTTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT1360.13578003634112737No Hit
CAACAAGGGAGGCCCTGGCTTCTAGAATGGAACAGAACACTGTCAGGGTTGTCCAGCCCACCTT1340.13378327110081667No Hit
CAACAAGGGGGCTATTTAAAACTCAGGAGTTCAAATAAACTCACAAATTGAACAGACACTGTTG1310.13078812324035063No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1300.12978974062019527No Hit
CAACAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGACGTCTT1230.12280106227910785No Hit
CAACAAGGGGGCAATGGCGTGATTTTATGGTTGGGGGTCAGCACAACTTGAGAAACTATATTAA1180.11780914917833109No Hit
CAACAAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC1160.1158123839380204No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1150.11481400131786507No Hit
CAACAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1140.11381561869770972No Hit
CAACAAGGGGTGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGC1130.11281723607755435No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC1130.11281723607755435No Hit
CAACAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA1130.11281723607755435No Hit
CAACAAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC1100.10982208821708832No Hit
CAACAAGGGGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATGGCTT1090.10882370559693298No Hit
CAACAAGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC1040.10383179249615622No Hit
CAACAAGGGGAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAATAT1030.10283340987600087No Hit
CAACAAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGC1030.10283340987600087No Hit
CAACAAGGGGTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCCC1030.10283340987600087No Hit
CAACAAGGGCATGCTGATAATGTACTGAGACTTCGGTATTTGACTCTATTTGTATCCTAGCAGC1020.10183502725584553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG150.004623032658.1630757
CACGCGA150.004623032658.1630758
ATGAACG150.004623032658.1630757
CTCACGG258.495015E-658.13390455
ACTCACG258.495015E-658.13390454
GCATCAT258.495015E-658.13390451
TTGATTC150.004632229558.133951
CTCACGC150.004632229558.133956
ATTACGA150.004632229558.133948
ATAGAGT150.004632229558.133953
CGACATA150.004632229558.133949
AATTGAC150.004632229558.133948
CATAGAG150.004632229558.133952
TACGAAA150.004632229558.133950
TACTGAT150.004632229558.133952
CTTTGTA150.004632229558.133956
ACGCTAC150.004632229558.133948
ACGTCAT201.9787558E-458.133950
TTACGAA150.004632229558.133949
ATTGACA150.004632229558.133949
Produced by FastQC (version 0.11.5)