FastQCFastQC Report
Fri 17 Jun 2016
SRR1526654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96265
Sequences flagged as poor quality0
Sequence length64
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC3000.31163974445540954No Hit
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.30332935126993193No Hit
CAACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG2610.2711265776762063No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2120.2202254194151561No Hit
CAACAAGGGGGAGGCACGGCCTCCGCCGTGTGGTCCAGCGGGGAAGGCTCCCTCTGTCTCTCTT2020.2098374279333091No Hit
CAACAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.2046434321923856No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.2004882355996468No Hit
CAACAAGGGGAGTGGGCTCTACTGGGCTCTCCTTGAGGGCCCCGGGTCTTCACACTCACGGTCC1910.1984106373032774No Hit
CAACAAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGAC1700.17659585519139873No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1670.17347945774684465No Hit
CAACAAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG1520.15789747052407418No Hit
CAACAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1400.14543188074585778No Hit
CAACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1390.14439308159767308No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1370.1423154833013037No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA1370.1423154833013037No Hit
CAACAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1360.141276684153119No Hit
CAACAAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1290.1340050901158261No Hit
CAACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1280.1329662909676414No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1130.11738430374487092No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1130.11738430374487092No Hit
CAACAAGGGAGGCCCTGGCTTCTAGAATGGAACAGAACACTGTCAGGGTTGTCCAGCCCACCTT1120.11634550459668623No Hit
CAACAAGGGGGCTATTTAAAACTCAGGAGTTCAAATAAACTCACAAATTGAACAGACACTGTTG1110.11530670544850152No Hit
CAACAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG1100.11426790630031684No Hit
CAACAAGGGGGCAATGGCGTGATTTTATGGTTGGGGGTCAGCACAACTTGAGAAACTATATTAA1020.10595751311483924No Hit
CAACAAGGGGTGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGC1010.10491871396665454No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC980.10180231652210045No Hit
CAACAAGGGGCTTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT970.10076351737391576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGA150.004596583558.24555235
ATGGGAT150.00461568358.18462439
ACACGAG150.00461568358.18462438
GACCATA150.00461568358.18462438
GCGCAAA150.00464444358.0934749
CCCCCGT150.00464444358.0934746
AATTCTC150.00464444358.0934750
GCGTGCA150.00464444358.0934747
TATATTA201.9903059E-458.06315657
CTCACGG351.6061676E-858.06315255
CCCGTCG258.553996E-658.06315252
CTCTCTA150.004654058758.06315257
TATGTCC150.004654058758.06315255
TTTCACG150.0046636958.03286443
ATCCTCC150.004682996357.9723825
ACCGCGG150.004682996357.9723841
TTAGAAC258.63378E-657.9723814
TAGAACC258.63378E-657.9723815
GCTTGAC150.004682996357.9723829
ACTAGCA150.004682996357.9723841
Produced by FastQC (version 0.11.5)