##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526654_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96265 Sequences flagged as poor quality 0 Sequence length 64 %GC 51 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.20851815301511 39.0 38.0 40.0 35.0 40.0 2 38.30514724977925 39.0 38.0 40.0 35.0 40.0 3 38.3938191450683 39.0 38.0 40.0 35.0 40.0 4 38.0631174362437 39.0 38.0 40.0 35.0 40.0 5 38.29735625616787 39.0 38.0 40.0 35.0 40.0 6 38.268404923907966 39.0 38.0 40.0 35.0 40.0 7 38.33740196333039 39.0 38.0 40.0 36.0 40.0 8 38.32738793954189 39.0 38.0 40.0 36.0 40.0 9 38.50041032566353 39.0 38.0 40.0 36.0 40.0 10 37.62755934140134 39.0 38.0 40.0 33.0 40.0 11 37.52188230405651 39.0 38.0 40.0 33.0 40.0 12 37.406648314548384 39.0 37.0 40.0 33.0 40.0 13 37.36948008102633 39.0 37.0 40.0 33.0 40.0 14 37.435121799200125 39.0 37.0 40.0 33.0 40.0 15 37.2028047577001 39.0 37.0 40.0 33.0 40.0 16 36.93887705812081 39.0 36.0 40.0 32.0 40.0 17 36.56540798836545 39.0 36.0 40.0 31.0 40.0 18 36.87424297512076 39.0 36.0 40.0 31.0 40.0 19 36.80247234197268 39.0 36.0 40.0 31.0 40.0 20 36.74628369604737 39.0 36.0 40.0 31.0 40.0 21 36.666046849841585 39.0 36.0 40.0 31.0 40.0 22 36.57612839557471 39.0 36.0 40.0 31.0 40.0 23 36.37806056199034 38.0 35.0 40.0 30.0 40.0 24 36.246538201838675 38.0 35.0 40.0 30.0 40.0 25 36.11794525528489 38.0 35.0 40.0 30.0 40.0 26 36.13931335376305 39.0 36.0 40.0 30.0 40.0 27 35.62080714693814 39.0 35.0 40.0 28.0 40.0 28 35.50478367007739 38.0 35.0 40.0 28.0 40.0 29 35.401641302654134 38.0 35.0 40.0 28.0 40.0 30 35.36067106424973 38.0 35.0 40.0 28.0 40.0 31 34.911255388770584 38.0 35.0 40.0 26.0 40.0 32 35.02387160442528 38.0 34.0 40.0 27.0 40.0 33 34.88977302238612 38.0 34.0 40.0 26.0 40.0 34 34.30179192853062 38.0 33.0 40.0 23.0 40.0 35 34.356598971588845 38.0 33.0 40.0 24.0 40.0 36 33.984397236794265 38.0 33.0 39.0 23.0 40.0 37 33.7913571910871 38.0 33.0 39.0 23.0 40.0 38 33.69809380356308 37.0 33.0 39.0 23.0 40.0 39 33.280081026333555 37.0 32.0 39.0 19.0 40.0 40 33.30856489897678 37.0 32.0 39.0 21.0 40.0 41 32.71361346283696 36.0 32.0 39.0 17.0 40.0 42 32.5383680465382 36.0 32.0 39.0 15.0 40.0 43 32.4058380512128 36.0 31.0 39.0 15.0 40.0 44 32.15936217732301 36.0 31.0 39.0 13.0 40.0 45 32.015987118890564 36.0 31.0 39.0 10.0 40.0 46 31.458474004051318 36.0 30.0 39.0 4.0 40.0 47 31.263366748039267 35.0 30.0 39.0 4.0 40.0 48 31.090458629823924 35.0 30.0 39.0 4.0 40.0 49 30.718464654858984 35.0 29.0 39.0 4.0 40.0 50 30.396031787253936 35.0 29.0 39.0 4.0 40.0 51 29.900763517373917 35.0 28.0 39.0 4.0 40.0 52 29.769885212694124 35.0 28.0 38.0 4.0 40.0 53 29.430447203033292 35.0 28.0 38.0 4.0 40.0 54 29.170217628421543 34.0 27.0 38.0 4.0 40.0 55 28.856043214044565 34.0 26.0 38.0 4.0 40.0 56 28.156380823767723 34.0 24.0 38.0 4.0 40.0 57 27.710039993767204 33.0 23.0 37.0 4.0 39.0 58 27.440035319171038 33.0 22.0 37.0 4.0 39.0 59 27.058868747727626 33.0 20.0 37.0 4.0 39.0 60 26.76945930504337 33.0 18.0 37.0 4.0 39.0 61 26.473557367682957 33.0 4.0 37.0 4.0 39.0 62 26.18480236846206 33.0 4.0 36.0 4.0 39.0 63 25.872414688619955 33.0 4.0 36.0 4.0 39.0 64 25.805703007323533 33.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.19940126801576952 2 2 0.8288794176480678 2 3 0.26085661481968714 2 4 0.6942948834977969 2 5 0.6377190862038233 2 6 0.6178521704328972 2 7 0.544447549864735 2 8 0.6167732696967221 2 9 0.5116765867771846 2 10 0.2813254852258851 2 11 -0.7816179108310521 2 12 -0.6849546388869214 2 13 -0.7576088104554017 2 14 -0.16135902005564162 2 15 0.19311111750013055 2 16 -0.07582246878889265 2 17 0.5487963283947863 2 18 -0.13456436442429265 2 19 -0.09961345327639037 2 20 0.2746348543140158 2 21 -0.19232632356577284 2 22 -0.1877567486602345 2 23 -0.22870739751711966 2 24 -0.06719583062903922 2 25 0.17906575977191608 2 26 -0.5400366103591026 2 27 -0.5147526532208246 2 28 -0.3343725772997743 2 29 -0.15260347736133184 2 30 -0.6855382064465516 2 31 0.5662032529780419 2 32 -0.11965638829271086 2 33 -0.08023606175635933 2 34 0.22895496516652258 2 35 0.250686192077076 2 36 -0.3489266853879158 2 37 -0.2624756803596924 2 38 -0.08187391944178302 2 39 -3.245945740964121 2 40 -0.5415493904554509 2 41 -0.894226376073977 2 42 -1.160981077013389 2 43 -1.423013194287865 2 44 -0.47241770598104793 2 45 -1.4404319968164963 2 46 -0.3612624998528453 2 47 -0.46194731224986896 2 48 -0.733066360619425 2 49 -0.5371824699212908 2 50 -0.2806941976555173 2 51 -0.32687101129438645 2 52 -0.41419619844799627 2 53 -0.17688223523437685 2 54 -0.7707989015747287 2 55 -1.2373543930596007 2 56 -0.5836964709118497 2 57 -1.7677677607585984 2 58 -1.1733515880455343 2 59 -1.5687687018954861 2 60 -1.7921863205925845 2 61 -1.4744983843367336 2 62 -1.9830212778614857 2 63 -2.2947355680817623 2 64 -1.7241118963411601 3 1 -0.5568289660133274 3 2 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30 0.7445693204351684 119 31 2.11572221166508 119 32 1.49494463038301 119 33 1.359039546619421 119 34 2.0080155197788656 119 35 1.271172892699603 119 36 0.7974230599431564 119 37 0.48591141641450264 119 38 0.5800389271909268 119 39 0.84030213679479 119 40 0.7183261047341389 119 41 -0.34233050736995807 119 42 -0.1267988472454178 119 43 0.28247633598944333 119 44 0.24031574053847748 119 45 0.2333653999011034 119 46 0.07722080518394137 119 47 1.2570113747903129 119 48 0.5002103796182666 119 49 0.6674581639213777 119 50 0.8623731481282988 119 51 0.3954832614843369 119 52 0.9536589232271595 119 53 1.0044420432036532 119 54 0.7338983253635831 119 55 1.3456676782477004 119 56 -0.3484955654223185 119 57 0.5549260705939751 119 58 0.44804060210726604 119 59 0.9524537089703884 119 60 0.7719336567701767 119 61 1.405637439092807 119 62 1.5875503124045025 119 63 0.8475394743630602 119 64 1.5963182111857215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 120.0 5 11.0 6 18.0 7 40.0 8 94.0 9 98.0 10 181.0 11 187.0 12 230.0 13 368.0 14 430.0 15 477.0 16 653.0 17 604.0 18 624.0 19 756.0 20 817.0 21 901.0 22 1123.0 23 1156.0 24 1188.0 25 1465.0 26 1495.0 27 1739.0 28 2021.0 29 2283.0 30 2786.0 31 3223.0 32 3864.0 33 4970.0 34 6188.0 35 8105.0 36 10190.0 37 13150.0 38 14774.0 39 9926.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.61251986744647 0.23788994733178895 0.06752334749592262 0.08206683772581366 8 99.56869225412859 0.20162337999771354 0.14030492937985223 0.08937943649383177 9 99.40476808809017 0.3677348984573833 0.09972471822573105 0.12777229522671793 10 64.12922661403417 22.99589674336467 4.313094063262868 8.561782579338285 11 33.220796758946655 30.675738845894145 16.060873630083623 20.04259076507557 12 33.38181062691529 17.007219654079883 26.224484495922713 23.38648522308212 13 23.89341920739625 17.52558042902405 31.613774476704933 26.967225886874775 14 26.125798576845167 19.50864800290864 30.97491300057134 23.390640419674856 15 26.68773848761164 19.01246633880577 29.526195947140227 24.773599226442364 16 27.426493506493504 20.534025974025973 25.576103896103895 26.46337662337662 17 28.539187952408685 20.6350361926949 23.015641900324486 27.810133954571924 18 28.725912844751466 22.015270347478317 22.433906404196748 26.824910403573472 19 27.170830519918976 21.378486469641096 22.66659741339012 28.784085597049806 20 27.724264056443985 21.124931159533237 22.667996716439625 28.482808067583154 21 28.858879135719107 20.458110424349453 24.228951332259907 26.45405910767153 22 28.831870357866308 19.818210149067678 23.84563444657975 27.50428504648626 23 30.67158364930141 21.37329247390017 23.256635329559032 24.698488547239393 24 28.883394281914892 22.75910073138298 22.300947473404257 26.056557513297875 25 28.404224356575607 21.009521433620222 23.688204232672238 26.898049977131926 26 29.037386479353273 20.291361000852053 24.239905234938384 26.431347284856294 27 26.898770419265166 18.423929452675196 25.419317223500503 29.25798290455913 28 28.053667523653885 19.73395230709981 25.73355677453603 26.478823394710272 29 27.684591692333306 19.73070839923416 25.817863980687587 26.766835927744943 30 25.710775893479916 19.593574648385854 27.838679117607256 26.85697034052698 31 26.422679553407765 20.726753874354277 28.141143142809533 24.70942342942843 32 28.467206460878796 19.743771204962222 29.285223653810128 22.503798680348854 33 29.474791228472064 18.80193256835836 28.33670005622774 23.386576146941835 34 26.492152057151795 21.33388955519633 31.308338113364968 20.865620274286904 35 25.58149224503401 23.053446246471466 31.020905596700104 20.344155911794424 36 26.6995402562472 20.728083984028856 30.107482042888567 22.46489371683538 37 26.126717971177708 23.10058603933346 29.165363198398296 21.607332791090535 38 27.31367090983017 22.050897111104163 30.622712914021204 20.012719065044465 39 28.50927649605723 20.88414315296283 29.55824217197599 21.048338179003952 40 26.06015727665255 20.348970609707766 30.65226006966897 22.938612043970714 41 24.094864464431044 21.65960643559191 32.82068404248659 21.42484505749045 42 23.674812030075188 19.357769423558896 31.186299081035923 25.781119465329994 43 24.60918327920552 18.1347312580278 33.34133937615522 23.91474608661146 44 22.758635087316417 19.864093276688134 33.62177847830398 23.755493157691465 45 24.83868273226554 19.80286925470378 32.77611877962704 22.582329233403637 46 25.402080374300272 19.690450330019218 32.12569972428774 22.781769571392765 47 23.92718765991666 19.98475243595501 32.89505289651499 23.19300700761334 48 25.914401762500916 19.725809988201267 32.27423177722323 22.085556472074593 49 23.879599360815483 20.20324396586838 34.22248216654308 21.694674506773058 50 22.63825426865609 20.24383358253432 33.35354823868924 23.76436391012035 51 22.653948220740958 21.180809494953717 32.47696261779886 23.688279666506467 52 23.594261053115563 22.506452657868063 30.277856150140547 23.621430138875827 53 22.943709370623523 22.057440270897345 31.813716268472646 23.18513409000648 54 24.45895814828361 22.295835728094467 30.10293118762736 23.142274935994568 55 24.208468782991353 21.509579905716585 30.058848738880933 24.223102572411126 56 24.309744597660295 23.015483048100947 29.084294272004012 23.590478082234746 57 25.112379518702042 21.401241924355517 29.903405883459826 23.582972673482615 58 23.97928761964538 22.87462733406559 28.863434280035566 24.282650766253468 59 24.12339428427968 23.219590777856812 30.424913176283525 22.232101761579983 60 24.226631504039517 23.923144543513754 27.69998744191888 24.150236510527858 61 25.48062347763363 23.124287820023625 28.157897487899476 23.23719121444327 62 24.423042737187732 22.707071974568915 27.970009724880008 24.899875563363345 63 24.202656070270837 24.12109170762313 27.622085119732304 24.054167102373732 64 24.778094121587674 22.75895997320382 27.1436945235304 25.31925138167811 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 2.5 3 5.0 4 3.0 5 1.0 6 1.0 7 10.5 8 20.0 9 36.0 10 77.0 11 102.0 12 113.5 13 237.5 14 350.0 15 481.0 16 616.5 17 621.0 18 748.0 19 875.0 20 1082.5 21 1391.5 22 1493.0 23 1723.0 24 1993.5 25 2034.0 26 1978.0 27 1797.5 28 1673.0 29 1562.5 30 1273.5 31 1095.0 32 1062.0 33 1029.0 34 1068.5 35 1049.5 36 991.0 37 1158.5 38 1362.0 39 1398.0 40 1332.5 41 1363.5 42 1460.0 43 1618.0 44 1776.0 45 1794.0 46 1856.0 47 1900.0 48 1940.0 49 2403.5 50 2827.0 51 2890.5 52 3518.5 53 4083.0 54 4636.0 55 5037.5 56 4886.0 57 5792.0 58 6698.0 59 6643.5 60 6388.5 61 6188.0 62 5893.5 63 5141.0 64 4683.0 65 4359.0 66 3531.5 67 3028.0 68 2648.0 69 2268.0 70 2067.5 71 1716.0 72 1565.0 73 1194.0 74 677.0 75 531.0 76 414.5 77 243.0 78 188.0 79 146.5 80 75.5 81 46.0 82 46.5 83 47.0 84 33.5 85 16.5 86 13.0 87 9.5 88 5.5 89 5.0 90 3.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.002077598296369397 8 0.04778476081649613 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08933672674388407 16 0.015581987222770478 17 0.11738430374487092 18 0.0 19 0.0 20 0.029086376149171558 21 0.0 22 0.0 23 0.0 24 0.009349192333662286 25 0.0633667480392666 26 0.02804757700098686 27 0.22438061600789488 28 0.1994494364514621 29 0.16724666285773646 30 0.21607022282241728 31 0.25866098789798997 32 0.18594504752506102 33 0.23580740663792657 34 0.3947436763101855 35 0.272165376824391 36 0.3552693086791669 37 0.3812392873837844 38 0.35942450527190567 39 0.6721030488755 40 0.39786007375473953 41 0.43941203968212744 42 0.5245935698332728 43 0.523554770685088 44 0.4820028047577001 45 0.510050381758687 46 0.5329039630187503 47 0.5318651638705656 48 0.5110891809068717 49 0.5380979587596738 50 0.6502882667636213 51 0.5723783306497688 52 0.5900379161689088 53 0.6056199033916793 54 0.5921155144652781 55 0.6191242923180803 56 0.6357450786890355 57 0.6305510829481119 58 0.695995429283748 59 0.6949566301355633 60 0.7365085960629512 61 0.6315898820962967 62 0.6585986599490988 63 0.6596374590972836 64 0.7572845790266453 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 96265.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.92816703890303 #Duplication Level Percentage of deduplicated Percentage of total 1 78.70517684493942 40.08310393185477 2 8.260922775670053 8.414273100296057 3 3.9101701138171583 5.974133901210201 4 2.3640517276547137 4.815872850984262 5 1.619548810835067 4.124032618293253 6 1.066780891771713 3.259751727003584 7 0.8342512136417412 2.974081961252792 8 0.5792844613062457 2.360151664675635 9 0.4303838779423163 1.9726795824027425 >10 2.080528699057643 18.298446995273466 >50 0.09790723289683026 3.521529112346128 >100 0.050993350467099095 4.201942554407105 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC 300 0.31163974445540954 No Hit CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 292 0.30332935126993193 No Hit CAACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG 261 0.2711265776762063 No Hit CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC 212 0.2202254194151561 No Hit CAACAAGGGGGAGGCACGGCCTCCGCCGTGTGGTCCAGCGGGGAAGGCTCCCTCTGTCTCTCTT 202 0.2098374279333091 No Hit CAACAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 197 0.2046434321923856 No Hit CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 193 0.2004882355996468 No Hit CAACAAGGGGAGTGGGCTCTACTGGGCTCTCCTTGAGGGCCCCGGGTCTTCACACTCACGGTCC 191 0.1984106373032774 No Hit CAACAAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGGAC 170 0.17659585519139873 No Hit CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG 167 0.17347945774684465 No Hit CAACAAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG 152 0.15789747052407418 No Hit CAACAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 140 0.14543188074585778 No Hit CAACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA 139 0.14439308159767308 No Hit CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA 137 0.1423154833013037 No Hit CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA 137 0.1423154833013037 No Hit CAACAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA 136 0.141276684153119 No Hit CAACAAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT 129 0.1340050901158261 No Hit CAACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA 128 0.1329662909676414 No Hit CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG 113 0.11738430374487092 No Hit CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG 113 0.11738430374487092 No Hit CAACAAGGGAGGCCCTGGCTTCTAGAATGGAACAGAACACTGTCAGGGTTGTCCAGCCCACCTT 112 0.11634550459668623 No Hit CAACAAGGGGGCTATTTAAAACTCAGGAGTTCAAATAAACTCACAAATTGAACAGACACTGTTG 111 0.11530670544850152 No Hit CAACAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG 110 0.11426790630031684 No Hit CAACAAGGGGGCAATGGCGTGATTTTATGGTTGGGGGTCAGCACAACTTGAGAAACTATATTAA 102 0.10595751311483924 No Hit CAACAAGGGGTGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGC 101 0.10491871396665454 No Hit CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC 98 0.10180231652210045 No Hit CAACAAGGGGCTTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCT 97 0.10076351737391576 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.002077598296369397 0.0 0.0 0.0 0.0 7 0.002077598296369397 0.0 0.0 0.0 0.0 8 0.002077598296369397 0.0 0.0 0.0 0.0 9 0.0062327948891081906 0.0 0.0 0.0 0.0 10 0.0062327948891081906 0.0 0.0 0.0 0.0 11 0.009349192333662286 0.0 0.0 0.0 0.0 12 0.009349192333662286 0.0 0.0 0.0 0.0 13 0.009349192333662286 0.0 0.0 0.0 0.0 14 0.010387991481846985 0.0 0.0 0.0 0.0 15 0.011426790630031684 0.0 0.0 0.0 0.0 16 0.01350438892640108 0.0 0.0 0.0 0.0 17 0.016620786370955175 0.0 0.0 0.0 0.0 18 0.017659585519139875 0.0 0.0 0.0 0.0 19 0.017659585519139875 0.0 0.0 0.0 0.0 20 0.02077598296369397 0.0 0.0 0.0 0.0 21 0.02077598296369397 0.0 0.0 0.0 0.0 22 0.02700877785280216 0.0 0.0 0.0 0.0 23 0.030125175297356255 0.0 0.0 0.0 0.0 24 0.03531917103827975 0.0 0.0 0.0 0.0 25 0.037396769334649145 0.0 0.0 0.0 0.0 26 0.03947436763101854 0.0 0.0 0.0 0.0 27 0.04259076507557264 0.0 0.0 0.0 0.0 28 0.050901158261050225 0.0 0.0 0.0 0.0 29 0.057133953150158415 0.0 0.0 0.0 0.0 30 0.06025035059471251 0.0 0.0 0.0 0.0 31 0.06025035059471251 0.0 0.0 0.0 0.0 32 0.06232794889108191 0.0 0.0 0.0 0.0 33 0.06440554718745131 0.0 0.0 0.0 0.0 34 0.065444346335636 0.0 0.0 0.0 0.0 35 0.07687113696566769 0.0 0.0 0.0 0.0 36 0.08206513270659119 0.0 0.0 0.0 0.0 37 0.08310393185477588 0.0 0.0 0.0 0.0 38 0.08622032929932998 0.0 0.0 0.0 0.0 39 0.08622032929932998 0.0 0.0 0.0 0.0 40 0.08829792759569938 0.0 0.0 0.0 0.0 41 0.09660832078117695 0.0 0.0 0.0 0.0 42 0.09972471822573105 0.0 0.0 0.0 0.0 43 0.10180231652210045 0.0 0.0 0.0 0.0 44 0.10803511141120864 0.0 0.0 0.0 0.0 45 0.11530670544850154 0.0 0.0 0.0 0.0 46 0.11634550459668623 0.0 0.0 0.0 0.0 47 0.12257829948579442 0.0 0.0 0.0 0.0 48 0.1267334960785332 0.0 0.0 0.0 0.0 49 0.12881109437490262 0.0 0.0 0.0 0.0 50 0.1319274918194567 0.0 0.0 0.0 0.0 51 0.1360826884121955 0.0 0.0 0.0 0.0 52 0.1371214875603802 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCCGA 15 0.0045965835 58.245552 35 ATGGGAT 15 0.004615683 58.184624 39 ACACGAG 15 0.004615683 58.184624 38 GACCATA 15 0.004615683 58.184624 38 GCGCAAA 15 0.004644443 58.09347 49 CCCCCGT 15 0.004644443 58.09347 46 AATTCTC 15 0.004644443 58.09347 50 GCGTGCA 15 0.004644443 58.09347 47 TATATTA 20 1.9903059E-4 58.063156 57 CTCACGG 35 1.6061676E-8 58.063152 55 CCCGTCG 25 8.553996E-6 58.063152 52 CTCTCTA 15 0.0046540587 58.063152 57 TATGTCC 15 0.0046540587 58.063152 55 TTTCACG 15 0.00466369 58.032864 43 ATCCTCC 15 0.0046829963 57.97238 25 ACCGCGG 15 0.0046829963 57.97238 41 TTAGAAC 25 8.63378E-6 57.97238 14 TAGAACC 25 8.63378E-6 57.97238 15 GCTTGAC 15 0.0046829963 57.97238 29 ACTAGCA 15 0.0046829963 57.97238 41 >>END_MODULE