FastQCFastQC Report
Fri 17 Jun 2016
SRR1526653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88810
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3710.41774574935255043No Hit
CAACAAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT2990.3366737979957212No Hit
CAACAAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2870.32316180610291634No Hit
CAACAAGGGGGAGGCACGGCCTCCGCCGTGTGGTCCAGCGGGGAAGGCTCCCTCTG2590.291633825019705No Hit
CAACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2360.26573584055849564No Hit
CAACAAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.2522238486656908No Hit
CAACAAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2130.23983785609728633No Hit
CAACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG1830.20605787636527417No Hit
CAACAAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT1810.20380587771647338No Hit
CAACAAGGGGAGTGGGCTCTACTGGGCTCTCCTTGAGGGCCCCGGGTCTTCACACT1790.20155387906767258No Hit
CAACAAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA1680.1891678864992681No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1670.1880418871748677No Hit
CAACAAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1660.1869158878504673No Hit
CAACAAGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC1530.172277896633262No Hit
CAACAAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1480.16664790001126No Hit
CAACAAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1440.16214390271365836No Hit
CAACAAGGGGGCTATTTAAAACTCAGGAGTTCAAATAAACTCACAAATTGAACAGA1320.1486319108208535No Hit
CAACAAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1270.14300191419885147No Hit
CAACAAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA1270.14300191419885147No Hit
CAACAAGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1270.14300191419885147No Hit
CAACAAGGGGAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATG1240.13962391622565026No Hit
CAACAAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA1180.13286792027924782No Hit
CAACAAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT1140.1283639229816462No Hit
CAACAAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1140.1283639229816462No Hit
CAACAAGGGAGGCCCTGGCTTCTAGAATGGAACAGAACACTGTCAGGGTTGTCCAG1120.1261119243328454No Hit
CAACAAGGGGCTTTTTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCT1040.11710392973764215No Hit
CAACAAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT1030.11597793041324175No Hit
CAACAAGGGGTGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGC1020.11485193108884136No Hit
CAACAAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA1020.11485193108884136No Hit
CAACAAGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1020.11485193108884136No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1000.11259993244004053No Hit
CAACAAGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC1000.11259993244004053No Hit
CAACAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC990.11147393311564013No Hit
CAACAAGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT980.11034793379123972No Hit
CAACAAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA970.10922193446683932No Hit
CAACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG950.1069699358180385No Hit
CAACAAGGGGGCAATGGCGTGATTTTATGGTTGGGGGTCAGCACAACTTGAGAAAC930.1047179371692377No Hit
CAACAAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT900.10133993919603648No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAACG150.00799696550.63360637
CAGTACG150.00799696550.63360637
AGGCTAG150.00799696550.63360637
CTAAACT251.9227986E-550.63360237
CTCTACG251.9227986E-550.63360237
GCCCACC150.0080151250.60458835
CCCACCA150.0080151250.60458836
AAGGCTA150.0080151250.60458836
GTGAAAC150.0080151250.60458836
GATAGTC150.0080151250.60458835
TCAGTAC150.0080151250.60458836
CCCGACA150.0080151250.60458836
AACTCTA251.9293393E-550.60458435
GAACACT251.9293393E-550.60458435
CGGGCAA150.00805152550.54665434
CACGTGA150.00805152550.54665434
GCCTACC150.00812470150.43118333
TCACGTG150.00812470150.43118333
CGTTAAA150.00814307250.402431
ATGCCTA150.00814307250.402431
Produced by FastQC (version 0.11.5)