FastQCFastQC Report
Fri 17 Jun 2016
SRR1526652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78899
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC7120.9024195490437141No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4980.6311867070558562No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4410.5589424454048847No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4210.5335935816677018No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4100.5196517066122511No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3320.4207911380372375No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3100.39290738792633617No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC3010.3815003992446038No Hit
CTCGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA2930.37136085374973066No Hit
CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA2500.3168607967147873No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT2070.26236073967984386No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGT1860.23574443275580173No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1740.22053511451349192No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCTCTTCCGAAGGTC1640.20786068264490046No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1500.19011647802887235No Hit
CTCGCAGGGGATTTTTAAAGCTCAAACCAAGTGACACTTTTAATAAGAGACTAAGATCTGATTG1480.18758159165515403No Hit
CTCGCAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1350.17110483022598513No Hit
CTCGCAGGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTGCGGCCGG1300.16476761429168937No Hit
CTCGCAGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC1290.16350017110483023No Hit
CTCGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1260.1596978415442528No Hit
CTCGCAGGGGCTCAAACCAAGTGACACTTTTAATAAGAGACTAAGATCTGATTGAGGGAGTTTT1230.15589551198367532No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTTTTCCAGAGGTCCT1190.15082573923623874No Hit
CTCGCAGGGAGCCGGAGGCCCCAGCGTGGCCGGCCCATTCTTCCCTGTGCCTCAGCCCAGCTGT1150.14575596648880212No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAAAGGTTT1120.1419536369282247No Hit
CTCGCAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG1100.1394187505545064No Hit
CTCGCAGGGGACGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGTGAGCGCTA1090.13815130736764725No Hit
CTCGCAGGGGCGCAGGGCCAAGGGCATGTTCACCGCTGAAGACCTGCGCTAGGGCTGCTCAGCC1060.13434897780706978No Hit
CTCGCAGGGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGAATGGCCATTGTCATAG1050.13308153462021063No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.12927920505963322No Hit
CTCGCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG990.12547687549905576No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC990.12547687549905576No Hit
CTCGCAGGGGTCCAGTTGACTTCACTTAGCTGTAGCTTACTCAGCATGACTGTAGATGAGGATA990.12547687549905576No Hit
CTCGCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.12420943231219661No Hit
CTCGCAGGGGAGCCTCGTGTGGAACTTGGAGACCATAGACACACTCAACCCCGGCTGCTCACTG960.1216745459384783No Hit
CTCGCAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC950.12040710275161914No Hit
CTCGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC950.12040710275161914No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGTTCC940.11913965956476003No Hit
CTCGCAGGGGGTACGCAACGTGGAGTATTATCAAAGCAACTACGTGTTCGTGTTTCTCGGCCTC920.11660477319104172No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC900.11406988681732341No Hit
CTCGCAGGGGCATGACTTCGCAGACCCCTTACTGGAAGCTGCCAAGATGGCCAATTATGCCAGA900.11406988681732341No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCT880.1115350004436051No Hit
CTCGCAGGGAACAAACCTCAGGAAAACTGTCCCTCTACCAGTTACCTTAGTTGGGGAGCCAGTG880.1115350004436051No Hit
CTCGCAGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAGTGCTTGAACGG870.11026755725674596No Hit
CTCGCAGGGGGCATTCTACAAAGTGACTTTGGAGGTGAGACCATGCCAGGACCCACATTTGATC850.10773267088302768No Hit
CTCGCAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC820.10393034132245023No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTC820.10393034132245023No Hit
CTCGCAGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT820.10393034132245023No Hit
CTCGCAGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCTCTTCCGAAGGTC820.10393034132245023No Hit
CTCGCAGGGGATTGTAAGGTTCAGGAAAAGAAGCGGCATCACCAAACGCTGTCAGTCATGTTTT810.10266289813559107No Hit
CTCGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC810.10266289813559107No Hit
CTCGCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT810.10266289813559107No Hit
CTCGCAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.10139545494873192No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCAAGGCCCC790.10012801176187278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATTA258.306E-658.3350458
CAGGCAC150.00456560658.3350458
AGGTCCG150.00456560658.3350458
GCAAGCC201.9487937E-458.297756
ATCAATA258.337638E-658.297754
CAATAAT258.337638E-658.297756
CTCAGAT150.004577207458.2976956
CCACATT150.004577207458.2976953
TATCTCA258.369374E-658.260448
AGAACGT150.004588830358.2603948
ATCGCCT150.004588830358.2603949
TGGTCAT150.004588830358.2603952
ATTATCT258.40121E-658.2231546
TAAGTTA150.004600475558.22314546
CAATCGC150.004600475558.22314547
AAAACAG201.9673885E-458.18594739
ATCACAA258.433148E-658.18594440
CACAATT258.433148E-658.18594442
TCGCATA150.004612142758.1859439
GTCGCAT150.004612142758.1859438
Produced by FastQC (version 0.11.5)