FastQCFastQC Report
Fri 17 Jun 2016
SRR1526651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75965
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC5210.6858421641545449No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT4970.6542486671493452No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC3950.5199763048772461No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3500.46073849799249655No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3200.42124662673599683No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3200.42124662673599683No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC2540.3343645099716975No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2450.32251694859474755No Hit
CTCGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA2280.3001382215493978No Hit
CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1940.2553807674586981No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGT1760.23168564470479827No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1660.21852168761929835No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCTCTTCCGAAGGTC1560.20535773053379844No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1320.17376423352859868No Hit
CTCGCAGGGGCTCAAACCAAGTGACACTTTTAATAAGAGACTAAGATCTGATTGAGGGAGTTTT1250.16454946356874875No Hit
CTCGCAGGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGTGCGGCCGG1220.1606002764430988No Hit
CTCGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC1200.1579674850259988No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1130.1487527150661489No Hit
CTCGCAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1080.14217073652339893No Hit
CTCGCAGGGGATTTTTAAAGCTCAAACCAAGTGACACTTTTAATAAGAGACTAAGATCTGATTG1080.14217073652339893No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCCAAAGGTTT1020.134272362272099No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTTTTCCAGAGGTCCT960.12637398802079905No Hit
CTCGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCC950.12505759231224906No Hit
CTCGCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.1197920094780491No Hit
CTCGCAGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC890.11715921806094913No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.11321003093529916No Hit
CTCGCAGGGGCGCAGGGCCAAGGGCATGTTCACCGCTGAAGACCTGCGCTAGGGCTGCTCAGCC860.11321003093529916No Hit
CTCGCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG850.11189363522674917No Hit
CTCGCAGGGAGCCGGAGGCCCCAGCGTGGCCGGCCCATTCTTCCCTGTGCCTCAGCCCAGCTGT820.10794444810109918No Hit
CTCGCAGGGGACGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGTGAGCGCTA800.10531165668399921No Hit
CTCGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC790.10399526097544921No Hit
CTCGCAGGGGAGCCTCGTGTGGAACTTGGAGACCATAGACACACTCAACCCCGGCTGCTCACTG780.10267886526689922No Hit
CTCGCAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAGGG780.10267886526689922No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC770.10136246955834924No Hit
CTCGCAGGGGTCCAGTTGACTTCACTTAGCTGTAGCTTACTCAGCATGACTGTAGATGAGGATA770.10136246955834924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGC150.004544524558.40106633
GATCGTA150.004544524558.40106636
ATTCGGG150.004544524558.40106637
TTACTGG150.004544524558.40106633
ATCGTAG150.004544524558.40106637
CGGATCG150.004544524558.40106634
CCTTTAG150.004544524558.40106633
GGATTCG150.004544524558.40106635
CCGTTCG303.5414814E-758.40106634
CGTAGTG150.004556535358.36218339
TATTAGT150.004556535358.36218338
TCGTAGT150.004556535358.36218338
TACGAAG258.41186E-658.20717254
AGGTCAT201.9633665E-458.2071758
GTCATAG150.00460481358.2071758
GTTTCGA150.00460481358.2071756
CATTGTC150.00460481358.2071754
TAGTTCG150.00460481358.2071754
TCATGAA150.00460481358.2071754
TCGTCCA150.00460481358.2071758
Produced by FastQC (version 0.11.5)