SRR1526650_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526650_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71346
Sequences flagged as poor quality	0
Sequence length	56
%GC	58

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	1012	1.4184397163120568	No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	584	0.8185462394528075	No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	543	0.7610798082583466	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	488	0.6839906932413871	No Hit
CTCGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	360	0.5045832982928264	No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	298	0.41768284136461753	No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	265	0.37142937235444173	No Hit
CTCGCAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	248	0.34760182771283604	No Hit
CTCGCAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	230	0.3223726627981947	No Hit
CTCGCAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	221	0.30975808034087404	No Hit
CTCGCAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA	214	0.2999467384296246	No Hit
CTCGCAGGGGACGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGT	189	0.26490623160373394	No Hit
CTCGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGATTGCTCTTC	172	0.24107868696212822	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC	169	0.23687382614302135	No Hit
CTCGCAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA	136	0.19062035713284556	No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC	131	0.18361225576766743	No Hit
CTCGCAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	129	0.18080901522159618	No Hit
CTCGCAGGGGCTCAAACCAAGTGACACTTTTAATAAGAGACTAAGATCTGATTGAG	118	0.16539119221820425	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	117	0.1639895719451686	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC	103	0.14436688812266982	No Hit
CTCGCAGGGAGCCGGAGGCCCCAGCGTGGCCGGCCCATTCTTCCCTGTGCCTCAGC	103	0.14436688812266982	No Hit
CTCGCAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	102	0.14296526784963418	No Hit
CTCGCAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	102	0.14296526784963418	No Hit
CTCGCAGGGGATTTTTAAAGCTCAAACCAAGTGACACTTTTAATAAGAGACTAAGA	98	0.13735878675749166	No Hit
CTCGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCC	96	0.1345555462114204	No Hit
CTCGCAGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTC	95	0.1331539259383848	No Hit
CTCGCAGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	93	0.1303506853923135	No Hit
CTCGCAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	93	0.1303506853923135	No Hit
CTCGCAGGGGGTACGCAACGTGGAGTATTATCAAAGCAACTACGTGTTCGTGTTTC	87	0.12194096375409975	No Hit
CTCGCAGGGGGGGTTGGAGAGATGGCTCAGTGGGTAAGAGCGCTGACTGCTCTTCC	86	0.12053934348106411	No Hit
CTCGCAGGGGAGCCTCGTGTGGAACTTGGAGACCATAGACACACTCAACCCCGGCT	85	0.11913772320802848	No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTTTTCCA	84	0.11773610293499286	No Hit
CTCGCAGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC	84	0.11773610293499286	No Hit
CTCGCAGGGCAGTACTTGAAGCTGAGCCTCTATGTGGGACTTTAAAAAAAAAAAAA	84	0.11773610293499286	No Hit
CTCGCAGGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGT	83	0.11633448266195723	No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCTG	82	0.11493286238892159	No Hit
CTCGCAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	82	0.11493286238892159	No Hit
CTCGCAGGGGCGCAGGGCCAAGGGCATGTTCACCGCTGAAGACCTGCGCTAGGGCT	81	0.11353124211588596	No Hit
CTCGCAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	79	0.1107280015698147	No Hit
CTCGCAGGGGGCATTCTACAAAGTGACTTTGGAGGTGAGACCATGCCAGGACCCAC	78	0.10932638129677906	No Hit
CTCGCAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA	76	0.10652314075070782	No Hit
CTCGCAGGGGTCCAGTTGACTTCACTTAGCTGTAGCTTACTCAGCATGACTGTAGA	75	0.10512152047767219	No Hit
CTCGCAGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCTCTTC	75	0.10512152047767219	No Hit
CTCGCAGGGATGATTGACCTGCCTCCTCCTACAGCGGGAGGCAGATCTGTCGGCAG	74	0.10371990020463656	No Hit
CTCGCAGGGAACAAACCTCAGGAAAACTGTCCCTCTACCAGTTACCTTAGTTGGGG	74	0.10371990020463656	No Hit
CTCGCAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA	73	0.10231827993160093	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACAC	20	3.8918297E-4	50.67191	37
ACAATAA	25	1.9102856E-5	50.67191	35
CAATAAC	25	1.9102856E-5	50.67191	36
GCAAAAC	15	0.007966536	50.671906	37
CCCCCCT	15	0.007966536	50.671906	37
TAAGGAC	15	0.007966536	50.671906	35
TTAAGGA	25	1.9183919E-5	50.635715	34
ATCAGAC	20	3.9055955E-4	50.63571	34
CCCAGTT	20	3.9055955E-4	50.63571	34
AATGCAA	20	3.9055955E-4	50.63571	34
CATTCAG	15	0.008079796	50.491455	33
TCGAGCA	15	0.008079796	50.491455	33
GATCAGA	20	3.9610468E-4	50.49145	33
TTGTGTC	15	0.008102591	50.455517	31
ACCGCTG	25	1.9593528E-5	50.455517	32
TTCACGG	30	9.710857E-7	50.455517	32
TCCTAAG	20	3.9750067E-4	50.455517	32
GCATTCA	15	0.008102591	50.455517	32
AGCATTC	15	0.008102591	50.455517	31
ATAAGTG	15	0.008102591	50.455517	32

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content